TY - JOUR T1 - Yapay Besin Ortamında Yetiştirilen Model Böcek Galleria mellonella’nın Farklı Yaşam Evrelerindeki Mikrobiyal Floranın Belirlenmesi TT - Determination of Microbial Flora of Model Insect Galleria mellonella in Different Life Forms Reared on Artificial Diet AU - Sugeçti, Serkan PY - 2021 DA - December DO - 10.7212/karaelmasfen.830759 JF - Karaelmas Fen ve Mühendislik Dergisi PB - Zonguldak Bülent Ecevit Üniversitesi WT - DergiPark SN - 2146-7277 SP - 132 EP - 137 VL - 11 IS - 2 LA - tr AB - Model böcekler düşük üretim maliyeti, etik sorunlar ve laboratuvar koşullarında kolay kültüre alınabilmesi nedeniyle tıp, eczacılıkve veterinerlik gibi birçok alanda omurgalı deney hayvanlarına alternatif deneysel model olarak kullanılmaktadır. Model böceklerinlaboratuvar koşullarında yapay besin ortamında üretilmesi sırasında en çok karşılaşılan sorun bakteri, maya ve küf kontaminasyonudur.Bu çalışmada model böcek Galleria mellonella’nın farklı yaşam evrelerinde dört gram negatif ve üç gram pozitif olmak üzere yedifarklı bakteri türü in vitro olarak tanımlandı. Mikrofungus cinsleri; Alternaria spp., Aspergillus spp., Beauveria sp., Penicillium spp. veVerticillium sp. olarak belirlendi. Yaşam evrelerinde en fazla koloni sayısı Aspergillus ve Penicillium cinsine ait türlerde tanımlanırken,en az koloni sayısının ise Verticillium cinsine ait olduğu belirlendi. Bu çalışma yapay besin ortamında mikrofloranın tanımlanması velaboratuvar koşullarında böcek üretim alanlarının standardize edilmesi açısından oldukça önemlidir. KW - Model böcek KW - mikrofungus KW - yapay besin KW - mikroflora KW - Galleria mellonella N2 - Model insects are used as an alternative experimental model to vertebrate experimental animals in many fields such as medicine,pharmacy and veterinary medicine due to their low production cost, ethical problems and easy culture in laboratory conditions.The most common problem encountered during the production of model insects in artificial diet under laboratory conditions isbacteria, yeast and mold contamination. In this study, seven different bacterial species, four gram negative and three gram positive, wereidentified in vitro in different life stages of the model insect Galleria mellonella. As microfungus genera Alternaria spp., Aspergillus spp.,Beauveria sp., Penicillium spp. and Verticillium sp. The highest number of colonies in their life stages is defined as the species belongingto the genus Aspergillus and Penicillium, while the least belong to the genus Verticillium determined. 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UR - https://doi.org/10.7212/karaelmasfen.830759 L1 - https://dergipark.org.tr/tr/download/article-file/1413367 ER -