TY - JOUR T1 - Kanatlıların IBV-Enfeksiyöz Bronşitis Hastalığına Karşı H-120 ve H-52 Suşları ile Canlı, Liyofilize, Pilot Aşı Hazırlanması TT - Preparation of live Lyophilized, pilot vaccine with H-52 strain against IBV Infections Bronchitis Disease of poultry AU - Bayraktar, Ragıp AU - Tayhanı, Canan AU - Zoğ, M. Zafer AU - Çiçek, Salih PY - 1995 DA - December JF - Etlik Veteriner Mikrobiyoloji Dergisi JO - J. Etlik Vet. Mic. PB - Veteriner Kontrol Merkez Araştırma Enstitüsü WT - DergiPark SN - 1016-3573 SP - 218 EP - 229 VL - 8 IS - 1 LA - tr AB - Bu araştırmada embriyolu SPF yumurtada liyofilize Mass. tipi H-120 ve H-52 Enfeksiyöz Bronşitis virüs suşları ile aşı üretilip, kanatlıların bu hastalığakarşı kullanılması amaçlanmıştır.Canlı, liyofilize, Mass. tipi H-120 ve H-521BV suşları 9-11 günlük embriyoluSPF yumurtaya corio :ıllantoik yolla in kule edilmiş, 36-48 saatler arası amnioallantoiksıvı toplanmış , sterilite ve titre kontrolleri yapıldıktan sonra %2-3oranında tindalize edilmiş süt tozu ilave edilmiş ve 1000 doz olacak şekildeampullere taksim edilerek liyofilize edilen ve -20 °C'Iik deep-freeze odalardasaklanmaktadır. (20,23)Aşılar şu anda istekler doğrultusunda +4 °C'de özel ambalajlarında tümyurda sevk edilmekte olup üretimimiz tüm ülkenin ihtiyacına cevap verecekdurumdadır. KW - Kanatlı KW - IBV-Enfeksiyöz Bronşitis Hastalığı KW - H-120 KW - H-52 KW - Ragıp BAYRAKTAR KW - Canan TAYHANI KW - M.Zafer ZOĞ KW - Salih ÇİÇEK KW - 1995 KW - Etlik Veteriner Mikrobiyoloji Dergisi N2 - The Study on live, Lyophilized IBV vaccines production by using IBV H-120 and H-52 strains.In this research; Vaccine production against to lnfections Bronchitis diseasehad been aimed by the live, freeze-dried , Mass. type H-120 and H-52IBV strains had been inoculated to 9-11 days old embriyoted SPF eggs by thecorio-allontoic route .After 36-48 hours, the virus content. 2-3 % tyndalizatedski m med m ilk powder is ad d ed to vaccine harvest and lyophilized . Lyophilizedvaccines are stored in the -20 oc deep-freeze room . (20 ,23)According to demands, the vaccines have been dispatching to all over thecountry, in the special boxes at +4°C. CR - A.J . BROWN. C. D. BRACEWEL, 1985: Application of the haemagglutination inhibition test to typhing of infectious brochitis virus. 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