@article{article_924278, title={Antiproliferative Activity of Pyrrolidine Derivatives compound in Colon Cancer Cells}, journal={Hitit Medical Journal}, volume={4}, pages={1–5}, year={2022}, DOI={10.52827/hititmedj.924278}, author={Mesci, Seda and Gül, Melek and Yıldırım, Tuba}, keywords={Antiproliferatif Aktivite, Kolon Kanseri, MTT, Pirolidin, RTCA}, abstract={<p> <b>Objective: </b> Anti-cancer drug research plays an important role for chemotherapeutic treatments in various types of cancer. Pyrolidine derived compounds have been reported by many researchers to be a potent anti-cancer compound. It is aimed to investigate the effects of pyrolidine-derived compounds that are thought to be new drug candidates with antiproliferative activity on DLD-1 and CCD-18CO cell lines. </p> <p> <b>Material and Methods: </b>The antiproliferative activity of the pyrrolidine-derived compound was determined for 24 hours at different concentrations (25-100 µM) on DLD-1 (human colon cancer) and CCD-18CO (normal colon fibroblast) cell lines by comparing MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5 Diphenyltetrazolium bromide) and RTCA (real-time cell analysis) assays. The significance of the differences between data sets in the MTT assay was analyzed statistically by ANOVA with SPSS 20.0 program for DLD-1 and CCD-18CO cell lines. </p> <p> <b>Results: </b> It has been determined that pyrrolidine-derived compounds reduce the number of DLD-1 cancer cells according to negative control with the MTT method and suppress the DLD-1 cell according to the RTCA assay results. Thus, the compounds have been shown to inhibit cell proliferation and have antiproliferative activity. </p> <p> <b>Conclusion: </b> Pyrrolidine-derived compounds will be the first step for antiproliferative activity studies in DLD-1 cancer cells and will guide the next studies. </p>}, number={1}, publisher={Hitit Üniversitesi}, organization={Amasya Üniversitesi}