@article{article_957791, title={Molecular Characterization of Gram Negative Isolated from Walnut (Juglans regia L.) Rhizosphere}, journal={Black Sea Journal of Engineering and Science}, volume={5}, pages={54–61}, year={2022}, DOI={10.34248/bsengineering.957791}, author={Erdem, Belgin and Sevim, Elif and Sevim, Ali}, keywords={Gram negetive bacteria, 16S rRNA sequence, Juglans regia L., Rhizosphere}, abstract={In this study, 10 Gram negative bacteria isolated from the rhizosphere soil of walnut (Juglans regia) orchard in the Kırşehir (Central Anatolia of Turkey) were identified at species level by classical and molecular methods. It was determined that 99% of phylogenetic similarities with bacterial isolates were closely related to the isolated Gram negative bacterial isolates. Ten isolates were morpho-biochemically and molecularly characterized and on the basis of 16S rDNA sequencing were identified as 3 Enterobacter cloacae, 3 Enterobacter ludwigii, 2 Escherichia coli, 1 Enterobacter hormaechei and 1 Plesiomonas shigelloides. Gram negative bacteria showed enzymes activities. Except for P. shigelloides and E. coli, other Gram negative bacteria showed lipase, protease, cellulase, amylase, gelatinase and pectinase activities at 4 °C and 28 °C. As a result, the current research suggests that strains isolated from walnut rhizosphere soil can be used as bio-fertilizing and bio-control agents.}, number={2}, publisher={Karyay Karadeniz Yayımcılık Ve Organizasyon Ticaret Limited Şirketi}, organization={Ahi Evran Üniversitesi, BİLİMSEL ARAŞTIRMA PROJELERİ KOORDİNASYON BİRİMİ}