@article{article_968583, title={Comparision of two identification methods: MALDI-TOF-MS & 16s rRNA sequencing in enterococci isolated from raw milk cheeses Sikma cheeses and evaluating their antibiotic susceptibility and antimicrobial activity}, journal={Türk Hijyen ve Deneysel Biyoloji Dergisi}, volume={77}, pages={399–412}, year={2020}, author={Aydın, Furkan and Kahve, Halil İbrahim and Ardıç, Mustafa and Çakır, İbrahim}, keywords={Gıda, Enterococcus, MALDI, sekanslama, antibiyotik direnci}, abstract={Objective: This study aims to identify enterococci isolated from squeezed cheeses by MALDI-TOF-MS and 16S rRNA sequence analysis and to evaluate antibiotic resistance and antibacterial effects of these isolates against some food pathogens.Methods: Identification of 84 Enterococcus isolates obtained from squeezed cheese was carried out using MALDI-TOF-MS and 16S rRNA gene sequencing. The isolates were tested for resistance to 14 different antibiotics by the disc diffusion method. The antimicrobial effects of isolates against various food pathogens were determined by the agar spot test. SPSS 22.0.0 software SPSS Inc., Chicago, USA was used for the assessment of the correlation between variance analysis ANOVA, F test and antibacterial results.Results: As a result of sequence analysis; 33 39.3% were described to E. faecalis, 29 34.5% to E. faecium, 14 16.7% to E. durans, 4 4.8% to E. gallinarum, 3 3.5% to E. casseliflavus and 1 1.2% to E. thailandicus. Eighty-one isolates gave the same identification result as the MALDI-TOF-MS method 96.43% . The difference between two identification methods has not been found to be statistically significant p>0.05 ; however, MALDI-TOF-MS has some advantages over 16S rRNA sequencing, such as being less labor-intensive, more economical and faster. In total, 83.3% of the strains exhibited multidrug-resistant phenotypes. A high incidence of resistance was detected for nalidixic acid, oxacillin, and streptomycin. E. faecalis isolates were found to show lower sensitivity to antibiotics tested than E. faecium p}, number={4}, publisher={T.C. Sağlık Bakanlığı Halk Sağlığı Genel Müdürlüğü}