@article{article_968586, title={An investigation of antifungal susceptibilities of the Candida species isolates from blood cultures using the Sensititre YeastOne microdilution method}, journal={Türk Hijyen ve Deneysel Biyoloji Dergisi}, volume={77}, pages={281–288}, year={2020}, author={Karaaslan, Raif and Aktaş, Esin and Orhan, Figen}, keywords={Antifungal, Mikrodilüsyon yöntemi, Sensititre YeastOne, Candida}, abstract={Objective: Invasive fungal infections caused by Candida species have increased significantly. Therefore, rapid and correct identification to the species level and susceptibility testing have become important, especially in cases involving invasive infections. The Sensititre YeastOne method, based on the microdilution method, is an alternative to the Clinical and Laboratory Standards Institute CLSI reference method for antifungal susceptibility testing. Sensititre YeastOne is a method for determining the Minimal Inhibitory Concentration MIC values of Candida isolates with wells containing lyophilized antifungals. In our study, the aim was to determine the antifungal drug sensitivity of Candida isolates from blood cultures by using a Sensititre YeastOne colorimetric system.Methods: Fifty candida isolates, each isolated from blood cultures of different patients in the Medical Microbiology Laboratory of Atatürk University Faculty of Medicine, were included in the study 16 Candida albicans, 14 Candida tropicalis, 8 Candida parapsilosis, 10 Candida glabrata, 2 Candida kefyr and two quality control strain Candida krusei ATCC 6258, Candida parapsilosis ATCC 22019 were included in the study. The antifungal susceptibilities of the species were tested by the microdilution method using a colorimetric method, Sensititre YeastOne Trek Diagnostic Systems, USA . Results: For all Candida species, MIC ranges were as follows: 0.12-2 μg/ml for amphotericin B; 0.12-256 μg/ml for fluconazole; 0.008-8 μg/ml for voriconazole; 0.015-0.5 μg/ml for caspofungin; 0.015-2 μg/ml for anidulafungin; 0.008-2 μg/ml for micafungin; 0.006-8 μg/ml flucytosine; 0.008-8 μg/ml for posaconazole and 0.015-16 μg/ml for itraconazole. Conclusion: Considering the low MIC values obtained, it was concluded that anidulafungin, micafungin, caspofungin, flucytosine, posaconazole, voriconazole, itraconazole and amphotericin B may be effective against Candida species and may be used as alternative drugs for the treatment of infections caused by fluconazole-resistant species.}, number={3}, publisher={T.C. Sağlık Bakanlığı Halk Sağlığı Genel Müdürlüğü}