TY  - JOUR
T1  - Nano Ozonized Oil Trigger ROS Production and ɣH2AX Cell Positivity of B-16 Melanoma and OV-90 Ovarian Cells
TT  - Nano Ozon Yağının B-16 Melanoma ve OV-90 Over Hücrelerinde ROS Üretimi ve ɣH2AX Hücre Pozitifliğini Tetiklemesi
AU  - Yalçın, Yeliz
AU  - Tekin, İshak Özel
AU  - Tığlı Aydın, Rahime  Seda
PY  - 2022
DA  - June
DO  - 10.7212/karaelmasfen.985125
JF  - Karaelmas Fen ve Mühendislik Dergisi
PB  - Zonguldak Bülent Ecevit Üniversitesi
WT  - DergiPark
SN  - 2146-7277
SP  - 38
EP  - 46
VL  - 12
IS  - 1
LA  - en
AB  - Tumor hypoxia is a restrictive factor for cancer treatment. Ozone therapy, used to decrease or prevent the hypoxia in tumor. In thisstudy, it is aimed to use ozonized oil nanoemulsions (OZNEs) to overcome the limitations of ozone therapy in cancer research. Theinfluence of OZNE against cancer cell lines is evaluated by Reactive Oxygen Species (ROS) and ɣH2AX assays. For both B-16melanoma and OV-90 cell lines, ROS production levels increased due to the increase in OZNE doses (0.85% to 14.88% and 1.94%to 58.58% for B-16 and OV-90 cell lines, respectively). 5-fold higher ɣH2AX cell positivity’s determined in OV-90 cells compared toB-16 melanoma cells. OZNE treatment could provide a new effective method to damage cancer cells in the future.
KW  - Ozonized oil
KW  - nano
KW  - ɣH2AX
KW  - ROS
KW  - B-16 melanoma
KW  - OV-90 ovarian
N2  - Tumör hipoksisi, kanser tedavisinde tedaviyi kısıtlayan bir faktördür. Ozon terapi, tumor hipoksisini azaltmak ya da önlemek amaçlıkullanılır. Bu çalışmada, kanser tedavisinde ozon terapinin kısıtlamalarının üstesinden gelmek için, ozon yağı nanoemülsiyonu(OZNE) kullanımı amaçlanmaktadır. OZNE’nin kanser hücre hatlarına olan etkisi, Reaktif Oksijen Türevleri (ROS) ve ɣH2AXtestleri ile değerlendirilmiştir. B-16 melanoma ve OV-90 over hücre hatlarının her ikisinde de, ROS üretimi OZNE doz artışı ileartmıştır (B-16 melanoma için %0.85’den %14.88’e, OV-90 over için %1.94’den %58.58’e), OV-90 over hücreleri ɣH2AX hücrepozitifliği B-16 melanom ile kıyaslandığında 5 kat fazla artış göstermiştir. OZNE tedavisi gelecekte kanser hücrelerine hasar verecekyeni etkili bir yöntem sağlayabilir.
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UR  - https://doi.org/10.7212/karaelmasfen.985125
L1  - https://dergipark.org.tr/tr/download/article-file/1934549
ER  -