Deneysel hipertiroidizmde α-lipoik asidin oksidatif stres parametreleri üzerine etkileri

Öz

Amaç: Deneysel hipertiroidizmde α-lipoik asid (ALA)’ in karaciğer dokusunda prooksidan-antioksidan denge üzerine etkileri ile karaciğer fonksiyon testlerinin incelenmesi.

Yöntem: Prooksidan-antioksidan dengenin değerlendirilmesi için, reaktif oksijen ürünleri (ROS), malondialdehit (MDA), protein karbonil (PC), total antioksidan kapasite (FRAP)  ve glutatyon (GSH) düzeyleri ile süperoksit dismutaz, katalaz ve glutatyon peroksidaz aktiviteleri incelendi.  Ayrıca, histopatolojik incelemeler yapıldı. Hipertiroidi tablosu oluşturmak için T4 (12 mg/L) 10 hafta boyunca içme suyunda uygulandı. ALA [100 mg/kg/gün; % 0.2 [w/w]; diyette] deney süresinin son 5 haftasında uygulandı.

Bulgular: Karaciğerde oksidatif stresin arttığı görüldü. Hipertiroidili sıçanlarda ROS, MDA, PK düzeylerinde anlamlı artış bulundu. Ayrıca FRAP düzeylerinde artış ve GSH düzeylerinde azalma görüldü. ALA tedavisi, artan serum serbest T3 ve T4 düzeylerini düşürdü ve karaciğerde ROS, MDA ve PK düzeylerini anlamlı olarak azalmasına neden oldu. ALA uygulaması öncesi ve sonrasında serum karaciğer fonksiyon testlerinde bir değişiklik görülmedi.

Sonuç: Sonuçlarımız, ALA tedavisinin prooksidan-antioksidan dengedeki değişikliklerin düzelmesinde etkili olduğunu ve hipertiroidi tedavisinde destekleyici ajan olarak yararlı olabileceğini göstermektedir. 

Anahtar Kelimeler

• prooksidan-antioksidan denge,hipertiroidizm,α-lipoik asid

Effects of α-lipoic acid on oxidative stress parameters in experimental hyperthyroidism

Abstract

Aim: To investigate the effects of α-lipoic acid (ALA) on prooxidant-antioxidant balance in liver tissue, as well as liver function tests in experimental hyperthyroidism.

Materials and Methods: For the evaluation of prooxidant-antioxidant balance, reactive oxygen species (ROS), malondialdehyde (MDA), protein carbonyl (PC), ferric reducing antioxidant power (FRAP), glutathione (GSH) levels, and superoxide dismutase, catalase and glutathione peroxidase activities were determined. Histopathological examinations were also performed. Hyperthyroidism was induced by the administration of L-thyroxine [T4, 12 mg/L]  in drinking water for 10 weeks. The ALA [100 mg/kg/day; 0.2% (w/w) in diet] was administered in last 5 weeks of experimental period. 

Results: Oxidative stress in liver tissue from hyperthyroid rats was accentuated. Significant increases in hepatic ROS, MDA, and PC levels were found. Additionally, increased FRAP and decreased GSH levels were observed.  ALA treatment lowered the elevated serum free T3 and T4 levels and significantly decreased hepatic ROS, MDA and PC levels. Serum liver function tests in hiperthyroid rats before and after ALA treatment were not changed.

Conclusion: Our results indicate that ALA treatment was effective in the improvement of changes in prooxidant-antioxidant balance,  and may be useful as supportive agent for the treatment of hypertyroidism.

Keywords

• Proxidant-antioxidant balance,hyperthyroidism,α-lipoic acid

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