Yıl 2019, Cilt 4 , Sayı 3, Sayfalar 113 - 117 2019-12-01

Kronik asetaldehit uygulaması ile uyarılan oksidatif streste sıçan karaciğerinde ve beyin dokularında N-asetilsistein ve taurinin koruyucu etkileri
Protective effects of N-acetylcysteine and taurine on oxidative stress induced by chronic acetaldehyde administration in rat liver and brain tissues

Zeynep Dicle Yıldız [1] , Adile Merve Baki [2] , Canan Başaran-Küçükgergin [3] , Pervin Vural [4] , Semra Doğru-Abbasoğlu [5] , Müjdat Uysal [6]


Amaç: Asetaldehit (AA), alkol metabolizmasının ana ürünlerinden biridir. AA'e maruz kalma birçok diyet ürününün yenmesi, sigara dumanı/otomobil egzozlarının solunması veya kozmetik ürünlerle temas yoluyla oluşabilir. AA birikimi oksidatif strese neden olur. Bu çalışmanın amacı kronik AA'e maruz kalan sıçanlarda prooksidant/antioksidan durumunu araştırmak ve N-asetil sistein (NAC) ve taurinin (TAU) proksidant/antioksidan dengesi üzerindeki etkilerini değerlendirmektir.

Yöntemler: Sprague Dawley sıçanlar aşağıdaki gruplara ayrıldı (n = 8; her biri): Kontrol, AA, AA+NAC, AA+TAU. Karaciğer ve beyin dokularında reaktif oksijen türleri (ROS), dien konjugatları (DC), malondialdehit (MDA), protein karbonil (PC), ferrik indirgeyici antioksidan güç (FRAP) ve glutatyon (GSH) düzeyleri ve ayrıca süperoksit dismutaz (SOD) ve glutatyon peroksidaz (GSH-Px) aktiviteleri incelendi.

Bulgular: İçme suyu ile AA uygulanan sıçanların karaciğer ve beyin dokularında prooksidan bir durum oluştuğu saptandı. NAC uygulaması her iki dokuda AA’e bağlı prooksidan durumu azalttı. TAU incelenen dokularda ROS oluşumunu azaltmasına rağmen, MDA ve PC düzeyleri değişmedi. NAC and TAU AA uygulanan sıçanların karaciğer ve beyinlerinde GSH düzeylerini arttırdı.

Sonuç: Kronik AA uygulamasının proksidant bir durum yarattığını, NAC/TAU uygulamalarının AA ile uyarılan oksidatif stresin baskılamada yararlı olabildiği görülmektedir.

Aim: Acetaldehyde (AA) is one of the main products of alcohol metabolism. Exposure to AA can occur through ingestion of several dietary products, inhalation of cigarette smoke/automobile exhausts, or contact with cosmetics. AA accumulation causes oxidative stress. The aim of this study was to investigate the prooxidant/antioxidant status in rats chronically exposed to AA, and to evaluate the effects of N-acetylcysteine (NAC) and taurine (TAU) on prooxidant/antioxidant balance.

Methods: Sprague Dawley rats were divided in the following groups (n=8; each): Control, AA, AA+NAC, AA+TAU. Reactive oxygen species (ROS), diene conjugate (DC), malondialdehyde (MDA), protein carbonyl (PC), ferric reducing antioxidant power (FRAP) and glutathione (GSH) levels as well as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities were determined in liver and brain tissues.

Results: AA treatment in drinking water was detected to induce prooxidant state in both liver and brain of rats. NAC treatment decreased AA-induced prooxidant status in both tissues. Although TAU treatment diminished ROS levels, MDA and PC levels remained unchanged in examined tissues of AA-treated rats. NAC and TAU elevated liver and brain GSH levels in AA-treated rats.

Conclusion: Chronic AA administration has created a prooxidant condition, and NAC/TAU appears to be useful in suppression of the developed oxidative stress.

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Konular Genel ve Dahili Tıp
Bölüm Orjinal Makale
Yazarlar

Orcid: 0000-0002-6518-0538
Yazar: Zeynep Dicle Yıldız
Kurum: ISTANBUL UNIVERSITY, İSTANBUL FACULTY OF MEDICINE, ISTANBUL MEDICINE PR.
Ülke: Turkey


Yazar: Adile Merve Baki

Orcid: 0000-0002-1797-5889
Yazar: Canan Başaran-Küçükgergin
Kurum: ISTANBUL UNIVERSITY, İSTANBUL FACULTY OF MEDICINE, ISTANBUL MEDICINE PR.
Ülke: Turkey


Orcid: 0000-0001-6462-7388
Yazar: Pervin Vural (Sorumlu Yazar)
Kurum: İSTANBUL FACULTY OF MEDICINE
Ülke: Turkey


Orcid: 0000-0003-3467-9763
Yazar: Semra Doğru-Abbasoğlu
Kurum: ISTANBUL UNIVERSITY, İSTANBUL FACULTY OF MEDICINE, ISTANBUL MEDICINE PR.
Ülke: Turkey


Orcid: 0000-0002-8802-8766
Yazar: Müjdat Uysal
Kurum: ISTANBUL UNIVERSITY, İSTANBUL FACULTY OF MEDICINE, ISTANBUL MEDICINE PR.
Ülke: Turkey


Destekleyen Kurum Research Fund of Istanbul University
Proje Numarası 33568 / 42685
Teşekkür The present work was supported by the Research Fund of Istanbul University (Project No: 33568 / 42685).
Tarihler

Yayımlanma Tarihi : 1 Aralık 2019

Bibtex @araştırma makalesi { acem579968, journal = {Archives of Clinical and Experimental Medicine}, issn = {}, eissn = {2564-6567}, address = {hırkai şerif mah. keçeci çeşmesi sok. doktorlar sitesi b blok 6/7 Fatih İstanbul Türkiye}, publisher = {Mustafa Hasbahçeci}, year = {2019}, volume = {4}, pages = {113 - 117}, doi = {10.25000/acem.579968}, title = {Protective effects of N-acetylcysteine and taurine on oxidative stress induced by chronic acetaldehyde administration in rat liver and brain tissues}, key = {cite}, author = {Yıldız, Zeynep Dicle and Baki, Adile Merve and Başaran-Küçükgergin, Canan and Vural, Pervin and Doğru-Abbasoğlu, Semra and Uysal, Müjdat} }
APA Yıldız, Z , Baki, A , Başaran-Küçükgergin, C , Vural, P , Doğru-Abbasoğlu, S , Uysal, M . (2019). Protective effects of N-acetylcysteine and taurine on oxidative stress induced by chronic acetaldehyde administration in rat liver and brain tissues. Archives of Clinical and Experimental Medicine , 4 (3) , 113-117 . DOI: 10.25000/acem.579968
MLA Yıldız, Z , Baki, A , Başaran-Küçükgergin, C , Vural, P , Doğru-Abbasoğlu, S , Uysal, M . "Protective effects of N-acetylcysteine and taurine on oxidative stress induced by chronic acetaldehyde administration in rat liver and brain tissues". Archives of Clinical and Experimental Medicine 4 (2019 ): 113-117 <https://dergipark.org.tr/tr/pub/acem/issue/50377/579968>
Chicago Yıldız, Z , Baki, A , Başaran-Küçükgergin, C , Vural, P , Doğru-Abbasoğlu, S , Uysal, M . "Protective effects of N-acetylcysteine and taurine on oxidative stress induced by chronic acetaldehyde administration in rat liver and brain tissues". Archives of Clinical and Experimental Medicine 4 (2019 ): 113-117
RIS TY - JOUR T1 - Protective effects of N-acetylcysteine and taurine on oxidative stress induced by chronic acetaldehyde administration in rat liver and brain tissues AU - Zeynep Dicle Yıldız , Adile Merve Baki , Canan Başaran-Küçükgergin , Pervin Vural , Semra Doğru-Abbasoğlu , Müjdat Uysal Y1 - 2019 PY - 2019 N1 - doi: 10.25000/acem.579968 DO - 10.25000/acem.579968 T2 - Archives of Clinical and Experimental Medicine JF - Journal JO - JOR SP - 113 EP - 117 VL - 4 IS - 3 SN - -2564-6567 M3 - doi: 10.25000/acem.579968 UR - https://doi.org/10.25000/acem.579968 Y2 - 2019 ER -
EndNote %0 Archives of Clinical and Experimental Medicine Protective effects of N-acetylcysteine and taurine on oxidative stress induced by chronic acetaldehyde administration in rat liver and brain tissues %A Zeynep Dicle Yıldız , Adile Merve Baki , Canan Başaran-Küçükgergin , Pervin Vural , Semra Doğru-Abbasoğlu , Müjdat Uysal %T Protective effects of N-acetylcysteine and taurine on oxidative stress induced by chronic acetaldehyde administration in rat liver and brain tissues %D 2019 %J Archives of Clinical and Experimental Medicine %P -2564-6567 %V 4 %N 3 %R doi: 10.25000/acem.579968 %U 10.25000/acem.579968
ISNAD Yıldız, Zeynep Dicle , Baki, Adile Merve , Başaran-Küçükgergin, Canan , Vural, Pervin , Doğru-Abbasoğlu, Semra , Uysal, Müjdat . "Protective effects of N-acetylcysteine and taurine on oxidative stress induced by chronic acetaldehyde administration in rat liver and brain tissues". Archives of Clinical and Experimental Medicine 4 / 3 (Aralık 2019): 113-117 . https://doi.org/10.25000/acem.579968
AMA Yıldız Z , Baki A , Başaran-Küçükgergin C , Vural P , Doğru-Abbasoğlu S , Uysal M . Protective effects of N-acetylcysteine and taurine on oxidative stress induced by chronic acetaldehyde administration in rat liver and brain tissues. ACEM. 2019; 4(3): 113-117.
Vancouver Yıldız Z , Baki A , Başaran-Küçükgergin C , Vural P , Doğru-Abbasoğlu S , Uysal M . Protective effects of N-acetylcysteine and taurine on oxidative stress induced by chronic acetaldehyde administration in rat liver and brain tissues. Archives of Clinical and Experimental Medicine. 2019; 4(3): 117-113.