Yıl 2019, Cilt 4 , Sayı 3, Sayfalar 118 - 121 2019-12-01

İnsan kaynaklı karaciğer (HepG2) hücrelerinde palmitat ile oluşturulan yağlanmanın paraoksonaz-1 ve paraoksonaz-3 enzimlerine etkisi
Effect of palmitate-induced steatosis on paraoxonase-1 and paraoxonase-3 enzymes in human-derived liver (HepG2) cells

Gülben Sayılan Özgün [1] , Eray Özgün [2] , Kıymet Tabakçıoğlu [3] , Selma Süer Gökmen [4] , Sevgi Eskiocak [5]


Amaç: Palmitat, hem sağlıklı bireylerin hem de non-alkolik karaciğer yağlanması hastalarının karaciğerinde en fazla bulunan yağ asitlerinden biridir. HepG2 hücrelerinde palmitat ile oluşturulan yağlanma, karaciğerdeki yağ birikiminin akut zararlı etkilerinin araştırılmasında kullanılan in vitro non-alkolik yağlı karaciğer hastalığı modelidir. Non-alkolik yağlı karaciğer hastalığı ateroskleroz ile yakından ilişkilidir. Paraoksonaz-1 ve paraoksonaz-3 dolaşımda yüksek dansiteli lipoproteine bağlı anti-aterosklerotik enzimlerdir ve esas olarak karaciğerde sentezlenirler. Palmitat ile oluşturulan yağlanmanın paraoksonaz-1 ve paraoksonaz-3 enzimleri üzerine etkisini araştıran bir çalışma bulunmamaktadır. Bu çalışmanın amacı HepG2 hücrelerinde palmitat ile oluşturulan yağlanmanın paraoksonaz-1 ve paraoksonaz-3 enzimlerine etkisini araştırmaktır.

Yöntemler: Yağlanma oluşturmak için hücreler 0.4, 0.7 ve 1 mM palmitat ile 24 saat inkübe edildi. Hücre canlılığı 3-(4,5-Dimetil-2-tiazolil)-2,5-difenil-2H-tetrazolium bromür testi ile değerlendirildi. Hücreler oil red O ile boyandı ve trigliserit düzeyleri ölçüldü. Paraoksonaz-1 ve paraoksonaz-3 protein düzeyleri western blot ile, mRNA’ları ise kantitatif PCR ile ve arilesteraz aktivitesi spektrofotometrik olarak ölçüldü.

Bulgular: Tüm palmitat konsantrasyonları paraoksonaz-1 mRNA düzeylerinde anlamlı bir artışa yol açtı. Palmitat konsantrasyonları paraoksonaz-1 ve paraoksonaz-3 protein düzeylerinde, paraoksonaz-3 mRNA düzeylerinde ve arilesteraz aktivitesinde anlamlı bir değişime yol açmadı.

Sonuç: Çalışmamız, HepG2 hücrelerinde palmitat ile oluşturulan yağlanmanın paraoksonaz-1 mRNA düzeyini arttırdığını, paraoksonaz-1 ve paraoksonaz-3 protein düzeylerine, paraoksonaz-3 mRNA düzeylerine ve arilesteraz aktivitesine etkisi olmadığını gösterdi.

Aim: Palmitate is one of the most abundant fatty acid in both liver of healthy individuals and in patients with non-alcoholic fatty liver disease. Palmitate-induced steatosis in HepG2 cells is an in vitro non-alcoholic fatty liver disease model to investigate acute harmful effects of fat overaccumulation in the liver. Non-alcoholic fatty liver disease is strongly associated with atherosclerosis. Paraoxonase-1 and paraoxonase-3 are anti-atherosclerotic enzymes which are bound to high density lipoprotein in circulation and they are primarily synthesized by liver. There is no study that investigated the effect of palmitate-induced steatosis on paraoxonase-1 and paraoxonase-3 enzymes. The aim of present study was to investigate the effect of palmitate-induced steatosis on paraoxonase-1 and paraoxonase-3 enzymes in HepG2 cells.

Methods: To induce steatosis, cells were incubated with 0.4, 0.7 and 1 mM palmitate for 24 hours. Cell viability was evaluated by 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay. Cells were stained with oil red O and triglyceride levels were measured. Paraoxonase-1 and paraoxonase-3 protein levels were measured by western blotting, their mRNA expression were measured by quantitative PCR and arylesterase activity was measured spectrophotometrically.

Results: All palmitate concentrations caused a significant increase on paraoxonase-1 mRNA levels. Palmitate concentrations did not cause a significant change on paraoxonase-1 and paraoxonase-3 protein levels, paraoxonase-3 mRNA levels and arylesterase activities.

Conclusion: Our study showed that palmitate-induced steatosis up-regulates paraoxonase-1 mRNA, has no effect on paraoxonase-1 and paraoxonase-3 protein levels, paraoxonase-3 mRNA and arylesterase activity in HepG2 cells.

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Orcid: 0000-0001-6990-3484
Yazar: Gülben Sayılan Özgün (Sorumlu Yazar)
Kurum: TRAKYA UNIVERSITY, FACULTY OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF MEDICAL BIOCHEMISTRY
Ülke: Turkey


Orcid: 0000-0002-6744-1519
Yazar: Eray Özgün
Kurum: TRAKYA UNIVERSITY, FACULTY OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF MEDICAL BIOCHEMISTRY
Ülke: Turkey


Orcid: 0000-0002-7345-0825
Yazar: Kıymet Tabakçıoğlu
Kurum: TRAKYA UNIVERSITY, FACULTY OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, MEDICAL DEPARTMENT OF BIOLOGY
Ülke: Turkey


Orcid: 0000-0001-5701-4962
Yazar: Selma Süer Gökmen
Kurum: TRAKYA UNIVERSITY, FACULTY OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF MEDICAL BIOCHEMISTRY
Ülke: Turkey


Orcid: 0000-0002-0813-2345
Yazar: Sevgi Eskiocak
Kurum: TRAKYA UNIVERSITY, FACULTY OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF MEDICAL BIOCHEMISTRY
Ülke: Turkey


Destekleyen Kurum No funding to declare.
Teşekkür None
Tarihler

Yayımlanma Tarihi : 1 Aralık 2019

Bibtex @araştırma makalesi { acem623975, journal = {Archives of Clinical and Experimental Medicine}, issn = {}, eissn = {2564-6567}, address = {hırkai şerif mah. keçeci çeşmesi sok. doktorlar sitesi b blok 6/7 Fatih İstanbul Türkiye}, publisher = {Mustafa Hasbahçeci}, year = {2019}, volume = {4}, pages = {118 - 121}, doi = {10.25000/acem.623975}, title = {Effect of palmitate-induced steatosis on paraoxonase-1 and paraoxonase-3 enzymes in human-derived liver (HepG2) cells}, key = {cite}, author = {Sayılan Özgün, Gülben and Özgün, Eray and Tabakçıoğlu, Kıymet and Süer Gökmen, Selma and Eskiocak, Sevgi} }
APA Sayılan Özgün, G , Özgün, E , Tabakçıoğlu, K , Süer Gökmen, S , Eskiocak, S . (2019). Effect of palmitate-induced steatosis on paraoxonase-1 and paraoxonase-3 enzymes in human-derived liver (HepG2) cells. Archives of Clinical and Experimental Medicine , 4 (3) , 118-121 . DOI: 10.25000/acem.623975
MLA Sayılan Özgün, G , Özgün, E , Tabakçıoğlu, K , Süer Gökmen, S , Eskiocak, S . "Effect of palmitate-induced steatosis on paraoxonase-1 and paraoxonase-3 enzymes in human-derived liver (HepG2) cells". Archives of Clinical and Experimental Medicine 4 (2019 ): 118-121 <https://dergipark.org.tr/tr/pub/acem/issue/50377/623975>
Chicago Sayılan Özgün, G , Özgün, E , Tabakçıoğlu, K , Süer Gökmen, S , Eskiocak, S . "Effect of palmitate-induced steatosis on paraoxonase-1 and paraoxonase-3 enzymes in human-derived liver (HepG2) cells". Archives of Clinical and Experimental Medicine 4 (2019 ): 118-121
RIS TY - JOUR T1 - Effect of palmitate-induced steatosis on paraoxonase-1 and paraoxonase-3 enzymes in human-derived liver (HepG2) cells AU - Gülben Sayılan Özgün , Eray Özgün , Kıymet Tabakçıoğlu , Selma Süer Gökmen , Sevgi Eskiocak Y1 - 2019 PY - 2019 N1 - doi: 10.25000/acem.623975 DO - 10.25000/acem.623975 T2 - Archives of Clinical and Experimental Medicine JF - Journal JO - JOR SP - 118 EP - 121 VL - 4 IS - 3 SN - -2564-6567 M3 - doi: 10.25000/acem.623975 UR - https://doi.org/10.25000/acem.623975 Y2 - 2019 ER -
EndNote %0 Archives of Clinical and Experimental Medicine Effect of palmitate-induced steatosis on paraoxonase-1 and paraoxonase-3 enzymes in human-derived liver (HepG2) cells %A Gülben Sayılan Özgün , Eray Özgün , Kıymet Tabakçıoğlu , Selma Süer Gökmen , Sevgi Eskiocak %T Effect of palmitate-induced steatosis on paraoxonase-1 and paraoxonase-3 enzymes in human-derived liver (HepG2) cells %D 2019 %J Archives of Clinical and Experimental Medicine %P -2564-6567 %V 4 %N 3 %R doi: 10.25000/acem.623975 %U 10.25000/acem.623975
ISNAD Sayılan Özgün, Gülben , Özgün, Eray , Tabakçıoğlu, Kıymet , Süer Gökmen, Selma , Eskiocak, Sevgi . "Effect of palmitate-induced steatosis on paraoxonase-1 and paraoxonase-3 enzymes in human-derived liver (HepG2) cells". Archives of Clinical and Experimental Medicine 4 / 3 (Aralık 2019): 118-121 . https://doi.org/10.25000/acem.623975
AMA Sayılan Özgün G , Özgün E , Tabakçıoğlu K , Süer Gökmen S , Eskiocak S . Effect of palmitate-induced steatosis on paraoxonase-1 and paraoxonase-3 enzymes in human-derived liver (HepG2) cells. ACEM. 2019; 4(3): 118-121.
Vancouver Sayılan Özgün G , Özgün E , Tabakçıoğlu K , Süer Gökmen S , Eskiocak S . Effect of palmitate-induced steatosis on paraoxonase-1 and paraoxonase-3 enzymes in human-derived liver (HepG2) cells. Archives of Clinical and Experimental Medicine. 2019; 4(3): 121-118.