acta med nicomedia Acta Medica Nicomedia 2717-8994 2717-8994 Kocaeli Üniversitesi 10.53446/actamednicomedia.1313876 Systems Biology Sistem Biyolojisi Nöropeptid W, kronik stresli sıçanlarda bilişsel bozukluğu ve kaygı benzeri davranışları hafifletir Neuropeptide W alleviates cognitive impairment and anxiety-like behaviors in rats with chronic stress https://orcid.org/0000-0003-2336-1450 Arabacı Tamer Sevil SAKARYA ÜNİVERSİTESİ, TIP FAKÜLTESİ, TEMEL TIP BİLİMLERİ BÖLÜMÜ, FİZYOLOJİ ANABİLİM DALI https://orcid.org/0009-0001-6731-099X Karahan Emine SAKARYA ÜNİVERSİTESİ, TIP FAKÜLTESİ, TIP PR. 10 21 2023 6 3 334 339 06 13 2023 09 14 2023 Copyright © 2020, Acta Medica Nicomedia 2020 Acta Medica Nicomedia

Amaç: NPW, strese karşı nöroendokrin yanıtta ve HPA ekseninin aktivasyonunda düzenleyici bir rol oynar. Bu çalışmanın amacı, kronik stres koşullarında periferik olarak uygulanan NPW'nin sıçanların bilişsel işlevler ve kaygı benzeri davranışlar üzerindeki etkilerini araştırmaktır.Yöntem: Wistar albino (250-290 gr) erkek sıçanlar kontrol, kronik stres grubu ve kronik strese maruz kalan ve NPW ile tedavi edilen (0.1 µg/kg, subkutan) grup olarak rastgele ayrıldı. Kronik stres indüksiyonu hayvanlara art arda on gün boyunca günde 1 saat sudan kaçınma stresi (WAS) uygulanarak yapıldı. Deneysel stres işleminin sonunda bilişsel işlevleri değerlendirmek için obje tanıma testi, kaygı düzeylerini değerlendirmek için delikli tahta testi kullanıldı. Deneylerden sonra, kortikosteron seviyelerini ölçmek için kan örnekleri alındı.Bulgular: Kontrol sıçanları ile karşılaştırıldığında WAS grubunda kortikosteron seviyesinde anlamlı artış görüldü (p

Objective: NPW plays a regulatory role in the neuroendocrine response to stress and the activation of the HPA axis. The current study aimed to investigate the effects of peripherally administered NPW on the cognitive functions and anxiety-like behavior of rats under chronic stress conditions.Methods: Wistar albino (250-290 gr) male rats were randomly divided as control, chronic stress group, and chronic stress exposed NPW-treated (0.1 µg/kg, subcutaneously) group. Chronic stress was induced by exposing the animals to water avoidance stress (WAS) for 1 h/day for ten consecutive days. At the end of the experimental stress procedure, an object recognition test was used to evaluate cognitive functions, and a hole-board test was used to assess anxiety levels. After the experiments, blood samples were collected to measure corticosterone levels.Results: WAS significant increases in the level of corticosterone when compared with control rats (p

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