acta med nicomedia Acta Medica Nicomedia 2717-8994 2717-8994 Kocaeli Üniversitesi Clinical Sciences (Other) Klinik Tıp Bilimleri (Diğer) Farelerde Pentilentetrazol ve Striknin ile Oluşturulan Konvülsiyonlar Üzerinde 7-Nitro İndazolün Etkisi The Effect of 7-Nitro Indazole on Strychnine and Pentylenetetrazole-Induced Seizures in Mice Erden Faruk KOCAELI UNIVERSITY, FACULTY OF MEDICINE, DEPARTMENT OF INTERNAL MEDICINE, DEPARTMENT OF PHARMACOLOGY Ulak Güner KOCAELI UNIVERSITY, FACULTY OF MEDICINE, DEPARTMENT OF INTERNAL MEDICINE, DEPARTMENT OF PHARMACOLOGY Göldeli Esin KOCAELI UNIVERSITY, FACULTY OF MEDICINE, DEPARTMENT OF INTERNAL MEDICINE, DEPARTMENT OF PHARMACOLOGY Gacar Nejat KOCAELI UNIVERSITY, FACULTY OF MEDICINE, DEPARTMENT OF INTERNAL MEDICINE, DEPARTMENT OF MEDICAL PHARMACOLOGY 11 30 1996 1 1 19 21 01 01 1996 Copyright © 2020, Acta Medica Nicomedia 2020 Acta Medica Nicomedia

Amaç:Nitrik oksidin (NO) santral fizyolojik olaylarda, hücrereler arası bir haberciolarak rolü olduğuna dair çeşitli kanıtlar mevcuttur. Çeşitli deneysel konvülsiyonmodellerinde nitrik oksid etkisiz, prokonvülsan veya antikonvülsan olarakbildirilmektedir. Bu çalışmada beyindeki nitrik oksid sentaz (NOS) tipinespesifik bir inhibitor olan 7-nitro indazolün (7-Nj), striknin (ST) vepentilentetrazol (PIZ) konvülsiyonları üzerindeki etkisini araştırmayı planladık.Yöntem:Subkütan (SK) ı.5 mg/kg ST veya 85 mg/kg PTZ verilerek; ilk miyoklonik spazm(jMS), toplam konvülsiyon süresi, ölüm zamanı ve ölüm oranları saptandı. 7 Nİ,AO veya serum fizyolojik (0. 1 ml/ 25gr) ST veya PTZ uygulamasından 30 dk önceintraperitoneal olarak uygulandı. 7-Nİ intraperitoneal (i.p.) kullanım içinyerfıstığı yağıında (arachis oil, AO; peanut oil) çözüldü. Bulgular:7-Ni (30 mg/kg) her iki tip konvülsiyon modelindeki tüm parametreler üzerindeetkisiz bulundu. Ancak AO ve SF alan fareler karşılaştırıldığında AO, STgruplarında tüm parametreleri, PTZ gruplarında ise sadece İMS'ı anlamlı olarakuzattı (p < 0,05). Sonuç:Bu sonuç muhtemelen i.p. verilen yerfıstığı yağının, daha sonra SK. verilen STve PTZ 'ün absorpsiyon ve/veya dağılım özelliklerini etkilemiş olabileceğiniakla getirmektedir. Sonuç olarak çalışmamızda kullanılan doz ve doz aralığında,7-Nİ bu tip konvülsiyonlar üzerinde etkisiz görülmektedir. 

Objective:There are some strong evidence about the role of nitric oxide (NO) as anintercellular messenger in the central physiological mechanisms. It issuggested that NO might be anticonvulsant, proconvulsant or ineffective on seizureactivity in various experimental seizure models. In this study, the effects of7-nitro indazole (7-NI), a selective inhibitor for the form of nitric oxidesynthase enzyme found in the brain, on strychnine (ST) and pentylenetetrazole(PTZ) induced seizures in mice were investigated. Methods:ST (1.5 mg/kg) or PTZ (85 mg/kg) were administered subcutaneously (s.c.) toproduce seizures. 7-NI, vehicle or saline (0. ı ml/25g, i.p.) were given 30minutes prior to ST or PTZ to separate mice groups. 7-NI(30mg/kg) was dissolvedin arachis oil (AO; peanut oil) for intraperitoneal (i.p.) administration. Results:The first myoclonic jerk (FMJ), the total convulsion time (TCT), the survivaltime, and the rate of mortality was recorded. 7-NI had no effect on allparameters, both in ST and PıZ induced seizures. Interestingly AO compared tosaline controls significantly delayed FMJ and survival time; increased TCT;decreased the rate of mortality induced by ST (p < 0.05). AO alsosignificantly delayed FMJ induced by PTZ compared to saline controls.Conclusion: It is very possible that the i.p.injection of AO could have altered absorption and/or distribution of thesubsequent s.c. injection of ST or PTZ. These observations suggested that 7-NI,at dose and time intervals used in this study, had no effect on seizureactivity.

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