Kerman Shahid Bahonar Üniversitesi Veteriner Hastanesi’ne Getirilen Köpeklerden Toplanan Uterus Örneklerinde Nested Trans-PCR ile C. burnetii Saptanması

Yıl 2018, Cilt: 44 Sayı: 1, 26 - 30, 16.01.2018

Mahdieh Rezaeı Mohammad Khalılı Farnoush Bakhshaee Shahrbabakı Zeinab Abırı

Öz

Tüm dünyada yaygın zoonotik bir hastalık olan Q-Humması’nın etkeni Coxiella burnetii’dir. Köpekler bu bakterinin
rezervuarıdır ve tüm eskretleri ile etkeni saçarak insanları ve diğer
hayvanları enfekte edebilirler. Bu çalışmada Kerman Shahid Bahonar Üniversitesi
Veteriner Hastanesi’ne
getirilen köpeklerin uterus örneklerinde Q-Humması etkeninin varlığını
belirledik. Örnekler toplanarak Nested Trans-PCR ile C. burnetii varlığı
değerlendirildi. Toplanan 41 örneğin 5’inde
(%12,1) etken moleküler düzeyde identifiye edildi. Bu çalışma, İran Kerman’da köpeklerin uterus
dokularında saptanmış ilk C. burnetii olgusudur. Bulgularımıza göre köpekler,
bu zoonoz hastalığın önemli bir kaynağıdır. Uterus örneklerinde etkenin
saptanması, bu bölgedeki küçük hayvanlarda Q-Humması’nın daha ayrıntılı bir şekilde araştırılması
gerekliliğini vurgulamaktadır. 

Anahtar Kelimeler

Q Humması, Coxiella burnetii, uterus örnekleri, nested trans-PCR, köpek, İran

Detection of C. burnetii in Uterine Samples Collected from Referred Dogs to the Veterinary Hospital of Shahid Bahonar University of Kerman by Nested Trans-PCR

Öz

Q fever, a universal zoonotic disease, is
caused by Coxiella burnetii. Dogs are considered as a reservoir of this
bacterium and can infect people and other animals by shedding this pathogen in
excretions. In this study, we determine the existence of the causative agent of
Q fever in the uterine samples of dogs attending to the veterinary animal
hospital of Shahid Bahonar University of Kerman. Specimens were collected and
assessed for the presence of C. burnetii by nested Trans-PCR. C. burnetii was
molecularly identified in 5 out of 41 (12.1%) collected samples. This study is
the first known identification of C. burnetii in the uterine of dogs in Kerman,
Iran. According to our findings, dogs should be considered as an important
source of this zoonotic pathogen. Detection of C. burnetii in uterine samples
emphasizes the need for the further investigations on Q fever in small animals
in this area. 

Anahtar Kelimeler

Q fever, Coxiella burnetii, uterine samples, nested trans-PCR, dog, Iran

Kaynakça

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