Araştırma Makalesi
BibTex RIS Kaynak Göster

Kerman Shahid Bahonar Üniversitesi Veteriner Hastanesi’ne Getirilen Köpeklerden Toplanan Uterus Örneklerinde Nested Trans-PCR ile C. burnetii Saptanması

Yıl 2018, Cilt: 44 Sayı: 1, 26 - 30, 16.01.2018

Öz

Tüm dünyada yaygın zoonotik bir hastalık olan Q-Hummasının etkeni Coxiella burnetiidir. Köpekler bu bakterinin
rezervuarıdır ve tüm eskretleri ile etkeni saçarak insanları ve diğer
hayvanları enfekte edebilirler. Bu çalışmada Kerman Shahid Bahonar Üniversitesi
Veteriner Hastanesi
ne
getirilen köpeklerin uterus örneklerinde Q-Humması etkeninin varlığını
belirledik. Örnekler toplanarak Nested Trans-PCR ile C. burnetii varlığı
değerlendirildi. Toplanan 41 örneğin 5
inde
(%12,1) etken moleküler düzeyde identifiye edildi. Bu çalışma, İran Kerman
da köpeklerin uterus
dokularında saptanmış ilk C. burnetii olgusudur. Bulgularımıza göre köpekler,
bu zoonoz hastalığın önemli bir kaynağıdır. Uterus örneklerinde etkenin
saptanması, bu bölgedeki küçük hayvanlarda Q-Humması
nın daha ayrıntılı bir şekilde araştırılması
gerekliliğini vurgulamaktadır. 

Kaynakça

  • Agerholm, J.S., 2013. C. burnetii associated reproductive disorders in domestic animals-a critical review. Acta Veterinaria Scandinavica 55, 1-11.
  • Asadi, J., Kafi, M., Khalili, M., 2014. Seroprevalence of C. burnetii in breeding rams and bucks, Online Journal of Veterinary Research 18, 415-419.
  • Berri, M., Laroucau, K., Rodolakis, A., 2000. The detection of Coxiella burnetii from ovine genital swabs, milk and fecal samples by use of a single touchdown polymerase chain reaction, Veterinary Microbiology 72, 285-93.
  • Buhariwalla, F., Cann, B., Marrie, T.J., 1996. A dog-related outbreak of Q fever. Clinical Infectious Diseases 23,753-5.
  • Cairns, K., Brewer, M., Lappin, M.R., 2007. Prevalence of C. burnetii DNA in vaginal and uterine samples from healthy cats of north-central Colorado. Journal of Feline Medicine and Surgery 9, 196-201.
  • Cantas, H., Muwonge, A., Sareyyupoglu, B., Yardimci, H., Skjerve, E., 2011. Q fever abortions in ruminants and associated on-farm risk factors in northern Cyprus. BMC veterinary research 7, 13.
  • Cooper, A., Hedlefs, R., Ketheesan, N., Govan, B., 2011. Serological evidence of C. burnetii infection in dogs in a regional centre. Australian Veterinary Journal 89, 385-387.
  • Ezatkhah, M., Alimolaei, M., Khalili, M., Sharifi, H., 2014. Seroepidemiological study of Q feverin small ruminants from Southeast Iran. Journal of Infection and Public Health 8, 170–176.
  • Fujishiro, M.A., Scorza, A.V., Gookin, J.L., Lappin, M.R., 2015. Evaluation of associations among C. burnetii and reproductive abnormalities in cats. Journal of feline medicine and surgery 5, 1098612X15584693.
  • Greene, C.E., 2012. Q fever (Chapter 46). In: Greene, C.E., Infectious Diseases of the Dogs and Cats. 4thed. Volume 2. St Louis. Elsevier Saunders, P: 482-484.
  • Gwida, M., El-Ashker, M., Khan, I., 2012. Q fever: a re-emerging disease?. Journal of Veterinary Science & Technology 3, 1-5.
  • Havas, K.A., Burkman, K., 2011. A Comparison of the Serological Evidence of C. burnetii Exposure between Military Working Dogs and Feral Canines in Iraq. Military medicine 176, 1101-1103.
  • Hornok, S., De´nes, B., Meli, M.L., Ta´nczos, B., Fekete, L., Gyuranecz, M., Fuente, J., Ferna´ndez de Mera, I.G., Farkas, R., Hofmann-Lehmann, R., 2013. Non-pet dogs as sentinels and potential synanthropic reservoirs of tick-borne and zoonotic bacteria. Veterinary Microbiology 167,700-3.
  • Khalili, M., Sakhaee, E., 2009. An Update on a Serologic Survey of Q Fever in Domestic Animals in Iran. American Journal of Tropical Medicine and Hygiene 80, 1031–1032.
  • Khalili, M., Sakhaee, E., Babaei, H., 2012. Frequency of anti-C. burnetii antibodies in cattle with reproductive disorders. Comparative Clinical Pathology 21, 917–919.
  • Khalili, M., Diali, H.G., Mirza, H.N., Mosavi, S.M., 2015. Detection of C. burnetii by PCR in bulk tank milk samples from dairy caprine herds in southeast of Iran. Asian Pacific Journal of Tropical Disease 5,119-22.
  • Khalili, M., Mosavi, M., Ghobadian Diali, H., Norouzian Mirza, H., 2014. Serologic survey for C. burnetii phase II antibodies among slaughterhouse workers in Kerman, southeast of Iran. Asian Pacific Journal of Tropical Biomedicine, 4, 209-212.
  • Khalili, M., Shahabi-Nejad, N., Golchin, M., 2010. Q fever serology in febrile patients in southeast Iran. Transactions of the Royal Society of Tropical Medicine and Hygiene 104, 623-624.
  • Kilic, S., Komiya, T., Celebi, B., Aydin, N., Saito, J., Toriniwa, H., Karatepe, B., Babur, C., 2008. Seroprevalence of C. burnetii in Stray Cats in Central Anatolia. Turkish Journal of Veterinary and Animal Sciences 32, 483-486.
  • Knobel, D.L., Maina, A.N., Cutler, S.J., Ogola, E., Feikin, D.R., Junghae, M., Halliday, J.E.B., Richards, A.L., Breiman, R.F., Cleaveland, S., Kariuki Njenga, M., 2013. C. burnetii in Humans, Domestic Ruminants, and Ticks in Rural Western Kenya. American Journal of Tropical Medicine and Hygiene 88, 513-518.
  • Komiya, T., Sadamasu, K., Kang, M., Tsuboshima, S., Fukushi, H., Hirai, K., 2003. Seroprevalence of Coxiella brnetii infections among cats in different living environment. Public health P: 1047-1048.
  • Kopecny, L., Bosward, K.L., Shapiro, A., Norris, J.M., 2013. Investigating C. burnetii infection in a breeding cattery at the centre of a Q fever outbreak. Journal of Feline Medicine and Surgery P: 1-9.
  • Marrie, T.J., MacDonald, A., Durant, H., Yates, L., McCormick, L., 1988. An outbreak of Q fever probably due to contact with a parturient cat. CHEST Journal 93, 98-103.
  • Marrie, T.J., Langille, D., Papukna, V., Yates, L., 1989. Truckin’pneumonia-an outbreak of Q fever in a truck repair plant probably due to aerosols from clothing contaminated by contact with newborn kittens. Epidemiology and Infection 102, 119-127.
  • Marrie, T.J., Durant, H., Williams, J.C., Mintz, E., Waag, D.M., 1988. Exposure to parturient cats: a risk factor for acquisition of Q fever in Maritime Canada. Journal of Infectious Diseases 158, 101-108.
  • Matthewman, L., Kelly, P., Hayter, D., Downie, S., Wray, K., Bryson, N., Rycroft, A., Raoult, D., 1997. Exposure of cats in southern Africa to C. burnetii, the agent of Q fever. European Journal of Epidemiology 13, 477-479.
  • Maywood, P., Boyd, R., 2011. Q fever in a small animal hospital. In: Australian College of Veterinary Scientists College Science Week Scientific Meeting: Epidemiology Chapter and Aquatic Animal Health Chapter Proceedings, 30 June–2 July, Gold Coast, Australia, p 4. Eight Mile Plains, Queensland: Australian College of Veterinary Scientists.
  • Mostafavi, E., Rastad, H., Khalili, M., 2012. Q fever: an emerging public health concern in Iran. Asian Journal of Epidemiology 5, 66.
  • Norris, J.M., Bosward, K.L., Heller, J., 2013. Q Fever: Pets, Vets And Validating a Tests. Microbiology Australia P: 186-188.
  • Parisi, A., Fraccalvieri, R., Cafiero, M., Miccolupo, A., Padalino, I., Montagna, C., Capuano, F., Sottili, R., 2006. Diagnosis of Coxiella burnetii-related abortion in Italian domestic ruminants using single-tube nested PCR. Veterinary Microbiology 118, 101-106.
  • Porter, S.R., Czaplicki, G., Mainil, J., Guatt´eo, R., Saegerman, C., 2011. Q Fever: Current State of Knowledge and Perspectives of Research of a Neglected Zoonosis. International Journal of Microbiology P: 1-22.
  • Roest, H.I.J., Solt, C.B., Tilburg, J.J.H.C., Klaassen, C.H.W., Hovius, E.K., Roest, F.T.F., Vellema, P., Brom, R., Zijderveld, F.G., 2013. Search for Possible Additional Reservoirs for Human Q Fever, the Netherlands. Emerging Infectious Diseases 9, 834-835.
  • Sakhaee, E., Khalili, M., 2010. The first serologic study of Q fever in sheep in Iran. Tropical Animal Health and Production 42, 1561-1564.
  • Skerget, M., Wenisch, C., Daxboeck, F., Krause, R., Haberl, R., Stuenzner, D., 2003. Cat or Dog Ownership and Seroprevalence of Ehrlichiosis, Q Fever, and Cat- Scratch Disease. Emerging Infectious Diseases 9, 1337-1340.

Detection of C. burnetii in Uterine Samples Collected from Referred Dogs to the Veterinary Hospital of Shahid Bahonar University of Kerman by Nested Trans-PCR

Yıl 2018, Cilt: 44 Sayı: 1, 26 - 30, 16.01.2018

Öz

Q fever, a universal zoonotic disease, is
caused by Coxiella burnetii. Dogs are considered as a reservoir of this
bacterium and can infect people and other animals by shedding this pathogen in
excretions. In this study, we determine the existence of the causative agent of
Q fever in the uterine samples of dogs attending to the veterinary animal
hospital of Shahid Bahonar University of Kerman. Specimens were collected and
assessed for the presence of C. burnetii by nested Trans-PCR. C. burnetii was
molecularly identified in 5 out of 41 (12.1%) collected samples. This study is
the first known identification of C. burnetii in the uterine of dogs in Kerman,
Iran. According to our findings, dogs should be considered as an important
source of this zoonotic pathogen. Detection of C. burnetii in uterine samples
emphasizes the need for the further investigations on Q fever in small animals
in this area. 

Kaynakça

  • Agerholm, J.S., 2013. C. burnetii associated reproductive disorders in domestic animals-a critical review. Acta Veterinaria Scandinavica 55, 1-11.
  • Asadi, J., Kafi, M., Khalili, M., 2014. Seroprevalence of C. burnetii in breeding rams and bucks, Online Journal of Veterinary Research 18, 415-419.
  • Berri, M., Laroucau, K., Rodolakis, A., 2000. The detection of Coxiella burnetii from ovine genital swabs, milk and fecal samples by use of a single touchdown polymerase chain reaction, Veterinary Microbiology 72, 285-93.
  • Buhariwalla, F., Cann, B., Marrie, T.J., 1996. A dog-related outbreak of Q fever. Clinical Infectious Diseases 23,753-5.
  • Cairns, K., Brewer, M., Lappin, M.R., 2007. Prevalence of C. burnetii DNA in vaginal and uterine samples from healthy cats of north-central Colorado. Journal of Feline Medicine and Surgery 9, 196-201.
  • Cantas, H., Muwonge, A., Sareyyupoglu, B., Yardimci, H., Skjerve, E., 2011. Q fever abortions in ruminants and associated on-farm risk factors in northern Cyprus. BMC veterinary research 7, 13.
  • Cooper, A., Hedlefs, R., Ketheesan, N., Govan, B., 2011. Serological evidence of C. burnetii infection in dogs in a regional centre. Australian Veterinary Journal 89, 385-387.
  • Ezatkhah, M., Alimolaei, M., Khalili, M., Sharifi, H., 2014. Seroepidemiological study of Q feverin small ruminants from Southeast Iran. Journal of Infection and Public Health 8, 170–176.
  • Fujishiro, M.A., Scorza, A.V., Gookin, J.L., Lappin, M.R., 2015. Evaluation of associations among C. burnetii and reproductive abnormalities in cats. Journal of feline medicine and surgery 5, 1098612X15584693.
  • Greene, C.E., 2012. Q fever (Chapter 46). In: Greene, C.E., Infectious Diseases of the Dogs and Cats. 4thed. Volume 2. St Louis. Elsevier Saunders, P: 482-484.
  • Gwida, M., El-Ashker, M., Khan, I., 2012. Q fever: a re-emerging disease?. Journal of Veterinary Science & Technology 3, 1-5.
  • Havas, K.A., Burkman, K., 2011. A Comparison of the Serological Evidence of C. burnetii Exposure between Military Working Dogs and Feral Canines in Iraq. Military medicine 176, 1101-1103.
  • Hornok, S., De´nes, B., Meli, M.L., Ta´nczos, B., Fekete, L., Gyuranecz, M., Fuente, J., Ferna´ndez de Mera, I.G., Farkas, R., Hofmann-Lehmann, R., 2013. Non-pet dogs as sentinels and potential synanthropic reservoirs of tick-borne and zoonotic bacteria. Veterinary Microbiology 167,700-3.
  • Khalili, M., Sakhaee, E., 2009. An Update on a Serologic Survey of Q Fever in Domestic Animals in Iran. American Journal of Tropical Medicine and Hygiene 80, 1031–1032.
  • Khalili, M., Sakhaee, E., Babaei, H., 2012. Frequency of anti-C. burnetii antibodies in cattle with reproductive disorders. Comparative Clinical Pathology 21, 917–919.
  • Khalili, M., Diali, H.G., Mirza, H.N., Mosavi, S.M., 2015. Detection of C. burnetii by PCR in bulk tank milk samples from dairy caprine herds in southeast of Iran. Asian Pacific Journal of Tropical Disease 5,119-22.
  • Khalili, M., Mosavi, M., Ghobadian Diali, H., Norouzian Mirza, H., 2014. Serologic survey for C. burnetii phase II antibodies among slaughterhouse workers in Kerman, southeast of Iran. Asian Pacific Journal of Tropical Biomedicine, 4, 209-212.
  • Khalili, M., Shahabi-Nejad, N., Golchin, M., 2010. Q fever serology in febrile patients in southeast Iran. Transactions of the Royal Society of Tropical Medicine and Hygiene 104, 623-624.
  • Kilic, S., Komiya, T., Celebi, B., Aydin, N., Saito, J., Toriniwa, H., Karatepe, B., Babur, C., 2008. Seroprevalence of C. burnetii in Stray Cats in Central Anatolia. Turkish Journal of Veterinary and Animal Sciences 32, 483-486.
  • Knobel, D.L., Maina, A.N., Cutler, S.J., Ogola, E., Feikin, D.R., Junghae, M., Halliday, J.E.B., Richards, A.L., Breiman, R.F., Cleaveland, S., Kariuki Njenga, M., 2013. C. burnetii in Humans, Domestic Ruminants, and Ticks in Rural Western Kenya. American Journal of Tropical Medicine and Hygiene 88, 513-518.
  • Komiya, T., Sadamasu, K., Kang, M., Tsuboshima, S., Fukushi, H., Hirai, K., 2003. Seroprevalence of Coxiella brnetii infections among cats in different living environment. Public health P: 1047-1048.
  • Kopecny, L., Bosward, K.L., Shapiro, A., Norris, J.M., 2013. Investigating C. burnetii infection in a breeding cattery at the centre of a Q fever outbreak. Journal of Feline Medicine and Surgery P: 1-9.
  • Marrie, T.J., MacDonald, A., Durant, H., Yates, L., McCormick, L., 1988. An outbreak of Q fever probably due to contact with a parturient cat. CHEST Journal 93, 98-103.
  • Marrie, T.J., Langille, D., Papukna, V., Yates, L., 1989. Truckin’pneumonia-an outbreak of Q fever in a truck repair plant probably due to aerosols from clothing contaminated by contact with newborn kittens. Epidemiology and Infection 102, 119-127.
  • Marrie, T.J., Durant, H., Williams, J.C., Mintz, E., Waag, D.M., 1988. Exposure to parturient cats: a risk factor for acquisition of Q fever in Maritime Canada. Journal of Infectious Diseases 158, 101-108.
  • Matthewman, L., Kelly, P., Hayter, D., Downie, S., Wray, K., Bryson, N., Rycroft, A., Raoult, D., 1997. Exposure of cats in southern Africa to C. burnetii, the agent of Q fever. European Journal of Epidemiology 13, 477-479.
  • Maywood, P., Boyd, R., 2011. Q fever in a small animal hospital. In: Australian College of Veterinary Scientists College Science Week Scientific Meeting: Epidemiology Chapter and Aquatic Animal Health Chapter Proceedings, 30 June–2 July, Gold Coast, Australia, p 4. Eight Mile Plains, Queensland: Australian College of Veterinary Scientists.
  • Mostafavi, E., Rastad, H., Khalili, M., 2012. Q fever: an emerging public health concern in Iran. Asian Journal of Epidemiology 5, 66.
  • Norris, J.M., Bosward, K.L., Heller, J., 2013. Q Fever: Pets, Vets And Validating a Tests. Microbiology Australia P: 186-188.
  • Parisi, A., Fraccalvieri, R., Cafiero, M., Miccolupo, A., Padalino, I., Montagna, C., Capuano, F., Sottili, R., 2006. Diagnosis of Coxiella burnetii-related abortion in Italian domestic ruminants using single-tube nested PCR. Veterinary Microbiology 118, 101-106.
  • Porter, S.R., Czaplicki, G., Mainil, J., Guatt´eo, R., Saegerman, C., 2011. Q Fever: Current State of Knowledge and Perspectives of Research of a Neglected Zoonosis. International Journal of Microbiology P: 1-22.
  • Roest, H.I.J., Solt, C.B., Tilburg, J.J.H.C., Klaassen, C.H.W., Hovius, E.K., Roest, F.T.F., Vellema, P., Brom, R., Zijderveld, F.G., 2013. Search for Possible Additional Reservoirs for Human Q Fever, the Netherlands. Emerging Infectious Diseases 9, 834-835.
  • Sakhaee, E., Khalili, M., 2010. The first serologic study of Q fever in sheep in Iran. Tropical Animal Health and Production 42, 1561-1564.
  • Skerget, M., Wenisch, C., Daxboeck, F., Krause, R., Haberl, R., Stuenzner, D., 2003. Cat or Dog Ownership and Seroprevalence of Ehrlichiosis, Q Fever, and Cat- Scratch Disease. Emerging Infectious Diseases 9, 1337-1340.
Toplam 34 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Cerrahi
Bölüm Original Article
Yazarlar

Mahdieh Rezaeı Bu kişi benim

Mohammad Khalılı Bu kişi benim

Farnoush Bakhshaee Shahrbabakı Bu kişi benim

Zeinab Abırı Bu kişi benim

Yayımlanma Tarihi 16 Ocak 2018
Yayımlandığı Sayı Yıl 2018 Cilt: 44 Sayı: 1

Kaynak Göster

APA Rezaeı, M., Khalılı, M., Shahrbabakı, F. B., Abırı, Z. (2018). Detection of C. burnetii in Uterine Samples Collected from Referred Dogs to the Veterinary Hospital of Shahid Bahonar University of Kerman by Nested Trans-PCR. Acta Veterinaria Eurasia, 44(1), 26-30.
AMA Rezaeı M, Khalılı M, Shahrbabakı FB, Abırı Z. Detection of C. burnetii in Uterine Samples Collected from Referred Dogs to the Veterinary Hospital of Shahid Bahonar University of Kerman by Nested Trans-PCR. Actavet. Ocak 2018;44(1):26-30.
Chicago Rezaeı, Mahdieh, Mohammad Khalılı, Farnoush Bakhshaee Shahrbabakı, ve Zeinab Abırı. “Detection of C. Burnetii in Uterine Samples Collected from Referred Dogs to the Veterinary Hospital of Shahid Bahonar University of Kerman by Nested Trans-PCR”. Acta Veterinaria Eurasia 44, sy. 1 (Ocak 2018): 26-30.
EndNote Rezaeı M, Khalılı M, Shahrbabakı FB, Abırı Z (01 Ocak 2018) Detection of C. burnetii in Uterine Samples Collected from Referred Dogs to the Veterinary Hospital of Shahid Bahonar University of Kerman by Nested Trans-PCR. Acta Veterinaria Eurasia 44 1 26–30.
IEEE M. Rezaeı, M. Khalılı, F. B. Shahrbabakı, ve Z. Abırı, “Detection of C. burnetii in Uterine Samples Collected from Referred Dogs to the Veterinary Hospital of Shahid Bahonar University of Kerman by Nested Trans-PCR”, Actavet, c. 44, sy. 1, ss. 26–30, 2018.
ISNAD Rezaeı, Mahdieh vd. “Detection of C. Burnetii in Uterine Samples Collected from Referred Dogs to the Veterinary Hospital of Shahid Bahonar University of Kerman by Nested Trans-PCR”. Acta Veterinaria Eurasia 44/1 (Ocak 2018), 26-30.
JAMA Rezaeı M, Khalılı M, Shahrbabakı FB, Abırı Z. Detection of C. burnetii in Uterine Samples Collected from Referred Dogs to the Veterinary Hospital of Shahid Bahonar University of Kerman by Nested Trans-PCR. Actavet. 2018;44:26–30.
MLA Rezaeı, Mahdieh vd. “Detection of C. Burnetii in Uterine Samples Collected from Referred Dogs to the Veterinary Hospital of Shahid Bahonar University of Kerman by Nested Trans-PCR”. Acta Veterinaria Eurasia, c. 44, sy. 1, 2018, ss. 26-30.
Vancouver Rezaeı M, Khalılı M, Shahrbabakı FB, Abırı Z. Detection of C. burnetii in Uterine Samples Collected from Referred Dogs to the Veterinary Hospital of Shahid Bahonar University of Kerman by Nested Trans-PCR. Actavet. 2018;44(1):26-30.