Immunohistochemical Evidence of Multiple Viral and Bacterial Associations in Caprine Pneumonia in Nigeria: Implications for Vaccines

Yıl 2019, Cilt: 45 Sayı: 1, 1 - 8, 01.01.2019

Theophilus Aghogho Jarıkre Benjamin Obukowho Emıkpe

Öz

DOI:
10.26650/actavet.2019.409009

Caprine pneumonia is a major cause of
economic loss and the conventional vaccines are not optimal in protecting
goats. A better understanding of the associations of respiratory pathogens may
help improve our knowledge for vaccination to effectively control caprine
pneumonia. One hundred and fifty goats (140 pneumonic and 10 normal) were
examined for various lung pathologies using standard gross and histologic
techniques. Antigens of parainfluenza 3 virus (PI3V), respiratory syncytial
virus (RSV) and peste des petits ruminants virus (PPRV) and bacterial antigens
of Mannheimia haemolytica (M.haemolytica) and Pasteurella multocida (P.
multocida) were demonstrated immunohistochemically in the lungs. The data of
goats positive and negative for the viral and bacterial antigens were analysed
using descriptive statistics.

Viral antigens were detected in 113 (81%) of
the pneumonic lungs (100 as single, 11 dual and 2 triple). Bacterial antigens
were detected in 120 (86%), M. haemolytica in 47 (34%),  P. multocida in 59 (42%) and combined
bacterial antigens in 14 (10%) of the pneumonic lungs. Multiple agents were
detected in 108/140 positive cases; virus-bacterium association was observed in
106/108. PPRV antigens alone were observed in 15 cases. PPRV coexisted most
frequently with M. haemolytica (n=20), P. multocida (n=13), PI3V with P.
multocida (n=18), and RSV with M. haemolytica (n=9). The lesions corresponded
to cranioventral (n=45), diffuse (n=75), and lobar consolidations (n=20)
manifested as fibrinous bronchopneumonia (n=22), suppurative bronchopneumonia
(n=20), bronchointerstitial pneumonia (n=61), interstitial pneumonia (n=25) and
bronchiolitis (n=12). Thus, multiple infections are involved in pneumonia,
hence we must consider combined vaccination strategies incorporating multiple
antigens for adequate control of caprine pneumonia. 

Anahtar Kelimeler

Goat pneumonia, pathogens, Pasteurella, Mannheimia, PI3V, PPRV, RSV

Kaynakça

• Ackermann, M.R. Brogden, K.A., 2000. Response of the ruminant respiratory tract to Mannheimia (Pasteurella) haemolytica review. Microbes and Infection 2, 1079-1088. Adeyemi, M.T., Morenikeji, O.A., Emikpe, B.O., Jarikre, T.A., 2017. Interactions between gastrointestinal parasitism and pneumonia in Nigerian goats. Journal of Parasitic Diseases 41, 726-733. Battaglia, R.A., 2001. Handbook of Livestock Management, 3rd ed. Prentice-Hall, Inc., New Jersey. Brogden, K.A., Lehmkuhi, H.D., Cutlip, R.C., 1998. Pasteurella haemolytica complicated respiratory infections in sheep and goats. Veterinary Research 29, 233-254. Brown, C.C., Mariner, C., Olander, H.J., 1991. An immunohistochemical study of the pneumonia caused by peste des petits ruminants virus. Veterinary Pathology 28, 166-170. Chakraborty, S., Kumar, A., Tiwari, R., Rahal, A., Malik, Y., Dhama, K., Pal, A., Prasad, M., 2014. Advances in diagnosis of respiratory diseases of small ruminants. Veterinary Medicine International 2014, Article ID 508304, 16 pages. Cirilo, E.C., Manchego, A.S., Rivera, H.H., 2012. Viral and bacterial coexistence in acute pneumonia in neonatal alpacas. La Revista de Investigaciones Veterinarias del Perú 23, 317-335. Cutlip, R.C., Lehmkuhl, H.D., Brogden, K.A., Hsu, N.J., 1996. Lesions in lambs experimentally infected with ovine adenovirus serotype 6 and Pasteurella haemolytica. Journal of Veterinary Diagnostic Investigation 8, 296-303. Dassanayake, R.P., Shanthalingam, S., Subramaniam, R., Herndon, C.N., Bavananthasivam, J., Haldorson, G.J., Foreyt, W.J, Evermann, J.F., Herrmann-Hoesing, L.M., Knowles, D.P., Srikumaran, S., 2013. Role of Bibersteinia trehalosi, respiratory syncytial virus, and parainfluenza-3 virus in bighorn sheep pneumonia. Veterinary Microbiology 162, 166-172. Davies, D.H., Herceg, M., Thurley, D.C., 1982. Experimental infection of lambs with an adenovirus followed by Pasteurella haemolytica. Veterinary Microbiology 7, 369-381. Emikpe, B.O., Akpavie S.O., 2011. The clinicopathologic effects of Peste des petits ruminants virus infection in West African dwarf goats. Small Ruminant Research 95, 168-173. Emikpe B.O., Akpavie, S.O., 2012. Clinicopathological observations in experimental Peste Des Petit Ruminants virus and Mannheimia haemolytica A:2 co-infection in goats. Nigerian Journal of Physiological Science 27, 129-136. Emikpe, B.O., Akpavie, S.O., Sabri, Y.M., Zamri-Saad, M., 2011. The effect of type of incubation and two different antigen retrieval methods on the immunodetection of Mannheimia hemolytica antigens in archived caprine lung tissues. Journal of Commonwealth Veterinary Association 27, 18-21. Emikpe, B.O., Jarikre, T.A., Eyarefe, O.D., 2013. Retrospective study of disease incidence and type of pneumonia in Nigerian small ruminants in Ibadan, Nigeria. African Journal of Biomedical Research 16, 107-113. Fulton, R.W., Purdy, C.W., Confer, A.W., Saliki, J.T., Loan, R.W., Briggs, R.E., Burge, L.J., 2000. Bovine viral diarrhea viral infections in feeder calves with respiratory disease: interactions with Pasteurella spp., parainfluenza- 3 virus, and bovine respiratory syncytial virus. Canadian Journal of Veterinary Research 64, 151-159. Gånheim, C., Hultén, C., Carlsson, U., Kindahl, H., Niskanen, R., Waller, K.P., 2003. The acute phase response in calves experimentally infected with bovine viral diarrhoea virus and/or Mannheimia haemolytica. Journal of Veterinary Medicine B, Infectious Diseases and Veterinary Public Health 50, 183-190. Gonzalez, C.T., Maheswaran, S.K., 1993. The role of induced virulence factors produced by Pasteurella haemolytica in the pathogenesis: review and hypothesis. British Veterinary Journal 149, 183-193. Guzmán, K.M., Rosadio, R.A, Maturrano, L.H., Manchego, A.S., 2013. Association of bacterial and viral agents in acute pneumonia in young alpacas. La Revista de Investigaciones Veterinarias del Perú 24, 524-536. Haritani, M., Nakazawa, M., Oohashi, S., Yamada, Y., Haziroglu, R., Narita, M., 1987. Immunoperoxidase evaluation of pneumonic lesions induced by Pasteurella haemolytica in calves. American Journal of Veterinary Research 48, 1358-1362. Hodgins, D.C., Conlon, J.A., Shewen PE., 2002. Respiratory Viruses and Bacteria in Cattle. In: Brogden KA, Guthmiller JM, (Eds). Polymicrobial Diseases. ASM Press, Washington (DC), pp. 450-462. Jarikre, T.A., Alao O.S., Emikpe B.O., 2018. Cultural and immunohistochemical evaluation of bacterial agents in caprine pneumonia in Nigeria. Comparative Clinical Pathology 27, 1051-1055. Jarikre, T.A., Emikpe, B.O., 2017. First report of immunohistochemical detection of peste des petit ruminants, parainfluenza 3 and respiratory syncytial viral antigens in lungs of Nigerian goats. Journal of Immunoassay and Immunochemistry 38, 555-568. Jarikre, T.A., Emikpe, B.O., Morenikeji, O.A., Akpavie S.O., 2016. Pattern and associated risk factors of caprine pneumonia in Nigeria. Asian Pacific Journal of Tropical Diseases 6, 179-183. Jasni, S., Zamri-Saad, M., Mutalib, A.R., Sheikh-Omar, A.R., 1991. Isolation of Pasteurella haemolytica from the nasal cavity of goats. British Veterinary Journal 147, 352-355. Kumar, P., Tripathi, B.N., Sharma, A.K., Kumar, R., Sreenivasa, B.P., Singh, R.P., Dhar, P., Bandyopadhyay, S.K., 2004. Pathological and immunohistochemical study of experimental peste des petits ruminants virus infection in goats. Journal of Veterinary Medicine, Series B 51, 153-159. Lacasta, D., Ferrer, L.M., Ramos, J.J., González, J.M., De las Heras, M., 2008. Influence of climatic factors on the development of pneumonia in lambs. Small Ruminant Research 80, 28-32. Lasisi, O.T., Ojo, N.A., Otesile, E.B., 2002. Estimation of age of cattle in Nigeria using rostral dentition. Tropical Veterinarian 20, 204-208. Lopez, A., 2012. Chapter 5: Respiratory system, Mediastinum and Pleurae. In: Zachary, J.F., McGavin, M.D., (Ed.), Pathologic Basis of Veterinary Disease. Mosby-Year Book Inc., Elsevier, pp. 458-538. Narita, M., Kimura, K., Tanimura, N., Arai, S., Tsuboi, T., Katsuda, K., 2000. Immunohistochemical characterization of calf pneumonia produced by the combined endobronchial administration of bovine herpesvirus 1 and Pasteurella haemolytica. Journal of Comparative Pathology 123, 126-134. Ozkul, A., Akca, Y., Alkan, F., Barrett, T., Karaoglu, T., Dagalp, S.B., Anderson, J., Yesilbag, K., Cokcaliskan, C., Gencay, A., Burgu, I., 2002. Prevalence, distribution and host range of peste des petits ruminants virus. Emerging Infectious Diseases 8, 708-712. Rosadio, R., Cirilo, E., Manchego, A., Rivera, H., 2011. Respiratory syncytial and parainfluenza type 3 viruses coexisting with Pasteurella multocida and Mannheimia hemolytica in acute pneumonias of neonatal alpacas. Small Ruminant Research 97, 110-116. Saliki, J.T., Brown, C.C., House, J.A., Dubovi, E.J., 1994. Differential immunohistochemical staining of peste des petits ruminants virus and rinderpest antigens in formalin-fixed, paraffin embedded tissues using monoclonal and polyclonal antibodies. Journal of Veterinary Diagnostic Investigation 6, 96-98. Sharma, R., Woldehiwet, Z., 1990. Increased susceptibility to Pasteurella haemolytica in lambs infected with bovine respiratory syncytial virus. Journal of Comparative Pathology 103, 411-420. Shoo, M.K., 1989. Experimental bovine pneumonic pasteurellosis: a review. Veterinary Record 124, 141-144. Yener, Z., Ilhan, F., Ilhan, Z., Saglam, Y.S., 2009. Immunohistochemical detection of Mannheimia (Pasteurella) haemolytica antigens in goats with natural pneumonia. Veterinary Research Communications 33, 305-313. Zamri-Saad, M., Jasni, S., Nurida, A.B., Sheikh-Omar, A.R., 1991. Experimental infection of dexamethasone treated goats with Pasteurella haemolytica A2. British Veterinary Journal 147, 565-568. Zamri-Saad, M., Maswati, M.A., Effendy, A.W.M., Salim, N.B., Sheikh-Omar, A.R., 1996. Establishment of Pasteurella haemolytica A2 in the lungs of goats following intratracheal exposure. Jurnal Veterinar Malaysia 8, 15-19. Zamri-Saad, M., Sharif, H., Basri, K., 1989. Microbiological and pathological evaluation of vaccination against naturally occurring caprine pasteurellosis. Veterinary Record 124, 171-172.