Yıl 2019, Cilt 45, Sayı 3, 101 - 107, 01.09.2019

Alper METE

Öz

Antibacterial Efficacy of Some Antiseptics and Disinfectants against Common Bacterial Agents Isolated from Horses in Turkey

Öz

DOI: 10.5152/actavet.2019.19022 Nowadays, many disinfectants and antiseptics are used for decontamination purposes in equine hospitals, on racetracks, and breeding farms, but generally, these antimicrobial agents are not tested against commonly encountered pathogens, and they are used with unknown antimicrobial efficacy. The antimicrobial efficacies of ethanol, chlorhexidine, povidone iodine, sodium hypochloride, peroxymonosulfate compound, and benzalkonium chloride were analyzed using the quantitative suspension test method against the field isolates of Escherichia coli, Pseudomonas aeruginosa, Salmonella spp., Streprococcus zooepidemicus, Streptococcus equi, Rhodococcus equi, and Staphylococcus auerus, which are the most frequently encountered pathogens of equines, in the presence of organic load (10% fetal bovine serum) after 1 min, 5 mins, and 30 mins contact times at 20°C. A log reduction of five or more (5 log ≤) in cfu counts of the tested pathogens was considered as effective for each disinfectant and antiseptic. According to the results, except for sodium hypochloride in the 1/100 dilution, all other disinfectants and antiseptics achieved a minimum 5 log reduction and were found to be effective against all tested isolates. Decreased dilutions and/or direct use of the sodium hypochloride should be tested against the same bacterial agents, as well as with multiple field strains. In addition, reference strains of the microorganisms should be evaluated in further studies. Cite this article as: Mete, A., 2019. Antibacterial Efficacy of Some Antiseptics and Disinfectants against Common Bacterial Agents Isolated from Horses in Turkey. Acta Vet Eurasia 45, 101-107.

Anahtar Kelimeler

Antiseptics, bacterial pathogens, biosecurity, disinfectants, horse

Kaynakça

• Addie, D.D., Corine, B.B., Egberink, H., Frymus, T., Jones, T.G., Hartmann, K., Horzinek, M.C., Hosie, M.J., Lloret, A., Lutz, H., Marisilio, F., Pennisi, M.G., Radford, A.D., Thiry, E., Truyen, U., Möstl, K., 2015. Disinfectant choices in veterinary practices, shelters and households ABCD guidelines on safe and effective disinfection for feline enviroments. Journal of Feline Medicine and Surgery 17, 594-605. Avcı, D., Otkun, M., 2017. Bazı antiseptik ve dezenfektanların antibakteriyel etkinliklerinin araştırılması. Türk Hijyen ve Deneysel Biyoloji Dergisi 74, 211-220. Bhosale, N.K., 2017. Evaluation and comparison of efficacy of the surfce disinfectants used in a tertiary care hospital, India. International Journal of Current Microbiology and Applied Sciences 6, 2608-2614. Chima, I.U., Uchegbu, M.C., Okoli, I.C., Ezema, C., Wehke, S.N., 2013. Evaluation of the efficacy of disinfectants used against bacterial isolates from intensive poultry farming environments in Imo State, Nigeria. Journal of Biological Sciences 13, 349-356. Dwyer, R.M., 1995. Disinfecting equine facilities. Revue Scientifique et Technique (International Office of Epizootics) 14, 403-418. Dwyer, R.M., 2004. Environmental disinfection to control equine infectious diseases. Veterinary Clinics Equine Practice 20, 531-542. El Aal, S.F.A.A., Hunsinger, B., Böhm, R., 2008. Determination of the bactericidal activity of chemical disinfectants against bacteria in dairies according to the DVG-guidelines. Hygiene Medical 33, 463-471. Fazlara, A., Ekhtelat, M., 2012. The disinfectant effects of benzalkonium chloride on some important foodborne pathogens. American-Eurasian Journal of Agricultural & Environmental Sciences 12, 23-29. Gehan, M.Z., Anwer, W., Amer, H.M., El-Sabagh, E.L., Rezk, A., Badawy, E.M., 2009. In vitro Efficacy comparisons of disinfectants used in the commercial poultry farms. International Journal of Poultry Science 8, 237-241. Ismail, E., Kaoud, H.A., Hamouda, M., Nermeen, A.E., 2015. Bactericidal effectiveness of some commerical disinfectants against Aeromonas hydrophila and Vibrio vulnificus. Ewemen Journal of Microbial Research 1, 9-15. Jang, Y., Lee, K., Lee, M., Song, J., Chang, B., Choe, N.H., 2017. Efficacy evaluation of commercial disinfectants by using Salmonella enterica serovar Typhimurium as a test organism. Journal of Veterinary Science 18, 209-216. Karayıldırım, Ç.K., Çelenk, V.U., 2016. Antibacterial efficiency of benzalkonium chloride base disinfectant according to European Standard 13727, chemical analysis and validation studies. Celal Bayar University Journal of Science 12, 39-47. Köse, H., Yapar, N., 2017. The comparison of various disinfectants’ efficacy on Staphylococcus aureus and Pseudomonas aeruginosa biofilm layers. Turkish Journal of Medical Sciences 47, 1287-1294. Morley, P.S., Morris, S.N., Hyatt, D.R., 2005. Evaluation of the efficacy of disinfectant footbaths as used in veterinary hospitals. Journal of American Veterinary Medicine Association 226, 2053-2058. Orji, N.M., 2014. Comparative efficacies of disinfectants against clinical and non clinical microorganisms. International Journal of Agriculture Innovations and Research 3, 155-156. Saklou, N.T., Burgess, B.A., Van Metre, D.C., Hornig, K.J., Morley, P.S., Byers, S.R., 2016. Comparison of disinfectant efficacy when using high-volume directed mist application of accelerated hydrogen peroxide and peroxymonosulphate disinfectants in a large animal hospital. Equine Veterinary Journal 48, 485-489. Sellon, D.C., Long, M.T., 2013. Equine Infectious Diseases (No. Ed. 2). Elsevier. Zareniya, M., Nezhadi, S.H., Dinmohamadi, F., Haghi, F., Hassan, M., 2017. Study the efficacy of antimicrobial activities of eight clinically applied disinfectants against clinical isolated of Enterococci and Pseudomonas aeruginosa. Journal of Pharmaceutical Sciences 23, 159-165.