İnsan Kronik Myeloid Lösemi Hücreleri Üzerine Florlu Shiff Bazı Ligandları ile Bunların Bakır ve Paladyum Komplekslerinin Antikanser Aktiviteleri

Abstract

Amaç: Kanser tedavisinde kemoterapatiklere bağlı yan etkilerin ortaya çıkması, ilaç direnci gelişimi en önemli sorunların başında gelmektedir. Bu güçlüklerin üstesinden gelebilmek için yeni antikanser ajanları araştırmalarına olan ihtiyaç artmaktadır. Son yıllarda Schiff bazlarının antikanser aktiviteye sahip olduğuna dair veriler dikkat çekmektedir. Bu bileşiklerin kanser hücrelerinde apoptozu indükleyerek hücre ölümüne yol açtığı ve DNA ve RNA sentezini durdurarak hücre çoğalmasını engellediği bildirilmektedir.Çalışmada, florlu Schiff bazları ile bunların bakır ve paladyum komplekslerinin insan kronik myeloid lösemi hücreleri üzerindeki sitotoksik etkileri araştırılmıştır. Araçlar ve Yöntem: K562 lösemi hücrelerinde florlu Schiff bazlarından o-florofenil-3,5-di-tert-butilsalisilaldimin [o-FPh-3,5-DTBS] ve p-florofenil-3,5-di-tert-butilsalisilaldimin [o-FPh-3,5-DTBS]) bileşiği ve bu bileşiklerin bakır ile paladyum komplekslerinin sitotoksik etkileri kolorimetrik olarak MTT yöntemiyle belirlendi. Bu amaçla sentezlenen altı bileşiğin 1-1000 μM aralığındaki logaritmik dozları kullanıldı. K562 hücrelerinin bileşiklerle 72 saat inkübasyondan sonra elde edilen optik dansite (OD) verilerinden yararlanılarak her bileşiğe ait IC50 değeri hesaplandı. Bulgular: K562 hücrelerde o-FPh-3,5-DTBS bileşiğinin, p-FPh-3,5-DTBS bileşiğine kıyasla daha yüksek sitotoksik etkiye sahip olduğu tespit edilmiştir. Aynı zamanda bu bileşiklerin bakır kompleksleri, paladyum komplekslerine göre daha güçlü sitotoksik etki göstermiştir. Sonuç: Sitotoksik etkiye sahip bu bileşikler ile yapılacak ileri düzeydeki çalışmaların faydalı olacağını düşünmektedir.

Keywords

• K562 hücre
• MTT yöntemi
• schiff baz
• Sitotoksisite

Anticancer Activities of Fluorinated Shiff Base Ligands and Their Copper and Palladium Complexes on Human Chronic Myeloid Leukemia Cells

Abstract

Purpose: The emergence of side effects due to chemotherapeutics in cancer treatment and the development of drug resistance are among the most important problems. In order to overcome these difficulties, the need for new anticancer agent research increases. In recent years, data indicating that Schiff bases have anticancer activity has attracted attention. It is reported that these compounds cause cell death by inducing apoptosis in cancer cells and prevent cell proliferation by stopping DNA and RNA synthesis. In the study, the cytotoxic effects of fluorinated Schiff bases and their copper and palladium complexes on human chronic myeloid leukemia cells were investigated. Materials and Methods: Cytotoxic effects of fluorine-containing Schiff bases (o-fluorophenyl-3,5-di-tert-butylsalicylaldimine [oFPh-3,5-DTBS] and p-fluorophenyl-3,5-di-tert-butylsalicylaldimine [o-FPh-3,5-DTBS]) and their copper and palladium complexes were determined colorimetrically by the MTT method. For this purpose, logarithmic doses of each of the six compounds synthesized in the range of 1-1000 μM were used. IC50values of each compound were calculated using the OD data obtained after 72 hours of incubation of K562 cells with the compounds. Results: The cytotoxic effect of o-FPh-3,5-DTBS on K562 cells was determined to be higher compared to p-FPh-3,5-DTBS. At the same time, copper complexes of Schiff bases showed stronger cytotoxic effects on K562 cells, while this effect was found to be weak in palladium complexes. Conclusion: We believe that further research on compounds with high cytotoxic effects may yield useful results.

Keywords

• Cytotoxicity
• K562 Cell
• MTT method
• schiff base

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