Passiflora Incarnata'nın Antikanser ve Antimikrobiyal Özellikleri

Abstract

Amaç: Passiflora incarnata, flavonoid ve fenolik içeriğine bağlı olarak terapötik özellikleriyle bilinen önemli bir tıbbi bitkidir. Bu çalışmanın amacı, P. incarnata'nın sitotoksik, oksidatif stres indükleyici, apoptotik ve antimikrobiyal etkilerini araştırmaktır. Araçlar ve Yöntem: Sitotoksisite, SNU-1 mide kanseri hücrelerinde XTT testi ile değerlendirilmiş ve IC₅₀ değeri 88.95 µg/mL olarak belirlenmiştir. Oksidatif stres belirteçleri, Total Antioksidan Kapasitesi (TAC) ve Total Oksidan Seviyesi (TOS) kitleri ile ölçülmüştür. Apoptotik aktivite, BAD ve Kaspaz-3 ELISA kitleri kullanılarak analiz edilmiştir. Antimikrobiyal etkiler, üç Gramnegatif bakteri (Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 700603 ve Pseudomonas aeruginosa ATCC 27853), iki Gram-pozitif bakteri (Staphylococcus aureus ATCC 29213 ve Enterococcus faecalis ATCC 29212) ve bir maya (Candida albicans ATCC 10231) olmak üzere altı mikroorganizma üzerinde agar disk difüzyon yöntemi ile test edilmiştir. İstatistiksel anlamlılık p<0.05 olarak kabul edilmiştir. Bulgular: P. incarnata, TAC seviyelerini anlamlı şekilde düşürmüş (p < 0.01) ve TOS seviyelerini artırmıştır (p < 0.01), bu da oksidatif stres geliştiğini göstermektedir. Ayrıca BAD ve Kaspaz-3 düzeylerini artırarak (p < 0.01) apoptozun uyarıldığını göstermiştir. İnhibisyon zonları şu şekilde ölçülmüştür: E. coli 12.27 mm, S. aureus 14.37 mm, K. pneumoniae 11.96 mm, P. aeruginosa 9.9 mm, E. faecalis 12.5 mm ve C. albicans 15.6 mm. Sonuç: P. incarnata, mide kanseri hücrelerinde oksidatif stres yoluyla apoptozu teşvik etmekte ve özellikle antifungal olmak üzere belirgin bir antimikrobiyal aktivite sergilemektedir. Bu özellikleri, antimikrobiyal dirence karşı daha fazla araştırmayı gerekli kılmaktadır.

Keywords

• Passiflora incarnata
• sitotoksisite
• oksidatif hasar
• apoptozis
• antimikrobiyal aktivite

Anticancer and Antimicrobial Properties of Passiflora Incarnata

Abstract

Purpose: Passiflora incarnata is a notable medicinal plant recognized for its therapeutic properties, largely attributed to its flavonoid and phenolic content. This study aimed to investigate the cytotoxic, oxidative stress-inducing, apoptotic, and antimicrobial effects of P. incarnata. Materials and Methods: Cytotoxicity was assessed using the XTT assay in SNU-1 gastric cancer cells, determining the IC₅₀ value as 88.95 μg/mL. Oxidative stress markers were evaluated via Total Antioxidant Capacity (TAC) and Total Oxidative Status (TOS) kits. Apoptotic activity was analyzed using BAD and Caspase-3 ELISA kits. Antimicrobial effects were tested against six microorganisms—three Gram-negative bacteria (Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 700603, and Pseudomonas aeruginosa ATCC 278538539), two Gram-positive bacteria (Staphylococcus aureus ATCC 29213 and Enterococcus faecalis ATCC 29212), and one yeast (Candida albicans ATCC 10231)—via the agar disk diffusion method. Statistical significance was defined as p<0.05. Results: P. incarnata significantly decreased TAS TAC levels (p < 0.01) and increased TOS levels (p < 0.01), indicating oxidative stress. It also elevated BAD and Caspase-3 levels (p < 0.01), suggesting apoptosis induction. Inhibition zones were E. coli 12.27 mm, S. aureus 14.37 mm, K. pneumoniae 11.96 mm, P. aeruginosa 9.9 mm, E. faecalis 12.5 mm, and C. albicans 15.6 mm Conclusion: P. incarnata promotes apoptosis through oxidative stress in gastric cancer cells and exhibits notable antimicrobial activity, particularly antifungal, warranting further investigation in combating antimicrobial resistance.

Keywords

• Passiflora incarnata
• cytotoxicity
• oxidative damage
• apoptosis
• antimicrobial activity.

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