Investigation of TheEffects of Trpc4/5 Inhibitor M084 in Experimental Alzheimer’s-LikeDementia Model

Abstract

Purpose: Vitiligo is a disorder of pigment loss caused by the progressive destruction of melanocytes. The pathophysiology of melanocyte loss has not been fully elucidated today. Oxidative stress is also among the causes. Dynamic thiol/disulfide homeostasis is an essential indicator of the body's oxidant-antioxidant balance. In this study, we evaluated the thiol/disulfide balance in vitiligo patients compared to healthy individuals.

Materials and Methods: Total thiol and native thiol levels and paraoxonase1 (PON1) activity were measured using a spectrophotometric method in the serum of 64 patients with vitiligo and 34 healthy individuals. Serum disulfide levels were calculated by calculation.

Results: Total and native thiol levels were higher in the serum of patients with vitiligo compared to healthy individuals (both p<0.001). There was no statistically significant difference in serum disulfide levels between the two groups.

Conclusion: The thiol/disulfide balance direction has shifted to the thiol direction in patients with vitiligo. Thiol increase may be responsible for the loss of melanin seen in the pathophysiology of the disease.

Keywords

• Thiol/disulfide homeostasis
• vitiligo
• oxidative stress

Vitiligoda Dinamik Tiyol/Disülfid Homeostazın Rolü

Abstract

Amaç: Vitiligo, melanositlerin progressif yıkımı sonucu oluşan pigment kaybı bozukluğudur. Melanosit kaybının patofizyolojisi günümüzde tam olarak çözülememiştir. Nedenler arasında oksidatif stres de yer almaktadır. Dinamik tiyol/disülfid homeostazı vücudun oksidan-antioksidan dengesinin önemli bir belirtecidir. Bu çalışmada vitiligo hastalarında sağlıklı bireylere göre tiyol/disülfid dengesini değerlendirdik.
Araçlar ve Yöntem: Vitiligolu 64 hasta ile 34 sağlıklı bireyin serumunda total tiyol ve native tiyol düzeyleri ile paraoxonaz1 (PON1) aktivitesi spektrofotometrik yöntemle ölçüldü. Serum disülfid düzeyleri ise hesaplamayla bulundu.
Bulgular: Vitiligolu hastaların serumunda sağlıklı kişilere göre total ve native tiyol düzeyleri yüksekti (her ikisi de p<0.001). Serum disülfid düzeylerinde ise her iki grup arasında istatistiksel olarak anlamlı fark yoktu.
Sonuç: Vitiligolu hastalarda tiyol/disülfid dengesinin yönü tiyol yönüne kaymıştır. Tiyol artışı hastalığın patofizyolojisinde görülen melanin kaybının sorumlusu olabilir. 

Keywords

• Tiyol/disülfid homeostazisi
• vitiligo
• oksidatif stres

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