Öz
The aim of this study was to identify hepatitis D virus genotypes in our chronic hepatitis D patients. Twenty-five patients with hepatitis D virus infection were enrolled in this study. HDV-RNA was extracted from the serum by Boom methods. Hepatitis D virus genotype was determined by reverse transcriptase-polymerase chain reaction (RT-PCR) and restriction fragment length polymorphism (RFLP) methods. Genotype I was found in 22 patients, and could not be determined in the remaining 3 patients. In conclusion, genotype I is the dominant hepatitis D virus genotype in our country.
Anahtar Kelimeler
Kaynakça
- Rizzetto M, Canese MG, Arico S, et al. Immunofluorescence detec- tion of new antigen-antibody system (d/anti-d) associated to hepati- tis B virus in liver and serum of HBsAg carriers. Gut 1977; 18: 997- 1003.
- M, Hoyer B, Purcell R, Gerin J. Hepatitis delta virus infection. In: Vyas G, Dienstag J, Hoofnagle J, eds. Viral Hepatitis and Liver Di- sease. New York: Grune & Stratton, 1984: 371-7.
- Boom R, Sol CJA, Salimans MMM, et al. Rapid and simple method for purification of nucleic acids. J Clin Microbiol 1990; 18: 495-503.
- Niro, G. A, A. Smedile, A. Andriulli, et al. The predominance of he- patitis delta virus genotype I among chronically infected Italian pa- tients. Hepatology. 1997; 25: 728-34.
- Ivaniushina V, Radjev N, Alexeeva M, et al. Hepatitis delta virus ge- notypes I and II cocirculate in an endemic area of Yakutia, Russia. J Gen Virol 2001; 82: 2709-18.
- Casey JL, Brown TL, Colan EJ, et al. A genotype hepatitis D virus that occurs in northren South America. Proc Natl Acad Sci USA 1993; 90: 9016-9020.
- Yurdaydin C. Kronik Delta Hepatiti ve Tedavisi. Gastroentero He- patoloji 2001; 12: 106-9.
Öz
Bu çalışmanın amacı; hepatitis D virusu ile infekte kronik hepatitli hastalarda genotip tayini yapmaktır. Çalışma kapsamına 25 hasta alınmıştır. HDV-RNA Boom metodu, hepatitis D virusu genotip tayini ise RT-PCR ve RFLP metodu ile çalışılmıştır. 22 hastada genotip I saptanmıştır. 3 hastada genotip tayini yapılamamıştır. Sonuç olarak; ülkemizdeki hepatitis D virusu, genotip I'dir.
Anahtar Kelimeler
Kaynakça
- Rizzetto M, Canese MG, Arico S, et al. Immunofluorescence detec- tion of new antigen-antibody system (d/anti-d) associated to hepati- tis B virus in liver and serum of HBsAg carriers. Gut 1977; 18: 997- 1003.
- M, Hoyer B, Purcell R, Gerin J. Hepatitis delta virus infection. In: Vyas G, Dienstag J, Hoofnagle J, eds. Viral Hepatitis and Liver Di- sease. New York: Grune & Stratton, 1984: 371-7.
- Boom R, Sol CJA, Salimans MMM, et al. Rapid and simple method for purification of nucleic acids. J Clin Microbiol 1990; 18: 495-503.
- Niro, G. A, A. Smedile, A. Andriulli, et al. The predominance of he- patitis delta virus genotype I among chronically infected Italian pa- tients. Hepatology. 1997; 25: 728-34.
- Ivaniushina V, Radjev N, Alexeeva M, et al. Hepatitis delta virus ge- notypes I and II cocirculate in an endemic area of Yakutia, Russia. J Gen Virol 2001; 82: 2709-18.
- Casey JL, Brown TL, Colan EJ, et al. A genotype hepatitis D virus that occurs in northren South America. Proc Natl Acad Sci USA 1993; 90: 9016-9020.
- Yurdaydin C. Kronik Delta Hepatiti ve Tedavisi. Gastroentero He- patoloji 2001; 12: 106-9.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Bölüm | Makaleler |
| Yazarlar | |
| Yayımlanma Tarihi | 1 Ağustos 2007 |
| Yayımlandığı Sayı | Yıl 2007 Cilt: 6 Sayı: 2 |
test-5