Abstract: Essential oil composition and Acetylcholinesterase (AChE) inhibition properties of Tripleurospermum inodorum
(L.) Sch. Bip. were investigated. Essential oils of flowers, leaves and stems were obtained through hydro-distillation
using a Clevenger type apparatus with 0.03, 0.02 and 0.01% (v/w) yields, respectively. Essential oil composition of
the oils was determined by GC-MS analyses. Total of ninety compounds were identified in flower oil comprising of
80.7% of the essential oil. The main components of the flower oil were artemisia ketone 14.4%, terpinen-4-ol 5.5%,
1,8-cineole 5.1%, sabinene 4.7% and tricosane 4.6%. Leaf oil was characterized by 35 compounds representing 62.7%
of the oil. Leaf oil contained caryophyllene oxide 16.0%, phytol 12.1%, spathulenol 5.9%, hexahydrofarnesyl acetone
3.8% and salvia-4(14)-en-1-one 3.5% compounds with high relative percentages. Stem essential oil comprised of
twenty-eight compounds representing 87.2% of the oil. The main components of the stem essential oil were were
neryl acetate 12.8%, (E)-β-farnesene 12.5%, phytol 12.1%, guaia-6,10(14)-dien-4β-ol 10.8%, γ-cadinol 7.8%, nonacosane
7.3%, decanoic acid 6.3% and caryophyllene oxide 4.6%. In vitro AchE inhition of the oils were evaluated by the
Ellman spectrophotometric method. AChE inhibitory property was investigated for the flower oil with at four different
concentrations. Highest AChE inhibitory property was observed for 20 mg/mL oil concentration (53.35 ± 1.37 %; n
=3). This AChE inhibitory concentration was equivalent to the activity of 1.26 μg galanthamine hydrobromide in the
assay. Activity of the essential oil was concentration dependent.
Tripleurospermum inodorum Essential oils AChE inhibition Artemisia ketone Caryophyllene oxide Neryl acetate
Birincil Dil | İngilizce |
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Bölüm | Research Article |
Yazarlar | |
Yayımlanma Tarihi | 30 Eylül 2018 |
Yayımlandığı Sayı | Yıl 2018 Cilt: 1 Sayı: 1 |