Gümüş Nitrat Katkılı Modifiye Nanofiber Membranların Hazırlanması, Karakterizasyonu ve Antibakteriyel Özellikleri

Yıl 2022, , 57 - 67, 28.02.2022

Burcu Özcan , Esengül Kır , Nalan Çiçek Bezir , Tuğba Sardohan Köseoğlu , İsmail Özmen , Atilla Evcin

https://doi.org/10.35414/akufemubid.866861

Öz

Son yıllarda, fonksiyonelleştirilmiş, elektro lif çekimi yöntemi ile hazırlanmış nanofiber membranlar ile antibakteriyel malzeme üretimi oldukça dikkat çekmektedir. Modifiye edilmiş nanofiber membranların üretiminde polimerik materyaller ile nanopartiküllerin kombinasyonu gerçekleştirilmektedir. Ag, Cu, Ce, ZnO ve TiO2 gibi birçok nanopartikülün kullanımı artmış ve antibakteriyel aktiviteleri üzerine çalışmaları hız kazanmıştır. Bu nedenle, bu çalışmada, elektro lif çekimi tekniği kullanılarak poliviniliden florür (PVDF) destek tabakası üzerine gümüş nitrat ilavesi ile modifiye nanofiber membranlar hazırlanmıştır. Kitosan (CS), polivinil alkol (PVA), polivinil prolidon (PVP) ve TiO2 gibi kimyasallar bu membranların hazırlanmasında kullanılmıştır. Modifiye nanofiber membranların karakterizasyonu ise taramalı elektron mikroskobu (SEM), enerji dağılım X-ışınları spektroskopisi (EDS) ve atomik güç mikroskobu (AFM) teknikleri kullanılarak gerçekleştirilmiştir. Karakterizasyon teknikleri, hazırlanan nanofiber membranların nano yapıda olduklarını kanıtlamıştır. Farklı miktarlarda AgNO3 içeren modifiye nanofiber membranların antibakteriyel özellikleri, seçilen E. coli (25922), S. aureus (ATCC 25923, ATCC 6538) ve P. aeruginosa (ATCC 35032) bakterilerine karşı iki farklı metot kullanılarak (Agar disk difüzyon ve yayma plak) test edilmiştir. Sonuç olarak, modifiye nanofiber membranların antibakteriyel aktiviteleri, gümüş iyonlarının ilavesi ile tüm bakterilere karşı artmıştır.

Anahtar Kelimeler

Antibakteriyel, Karakterizasyon, Membran, Nanofiber

Destekleyen Kurum

-

Proje Numarası

-

Preparation, Characterization and Antibacterial Properties of Silver Nitrate Doped Modified Nanofiber Membranes

Öz

In recent years, production of antibacterial material using functionalized nanofiber membranes prepared by electro-spinning method has attracted much attention. In the production of modified nanofiber membranes, the combination of polymeric materials with nanoparticles has been performed. The use of many nanoparticles such as Ag, Cu, Ce, ZnO and TiO2 has increased and studies on their antibacterial activities have accelerated. Therefore, in this study, modified nanofiber membranes were prepared by adding silver nitrate on the polyvinylidene fluoride (PVDF) support layer using electrospinning technique. Chitosan (CS), poly(vinyl alcohol) (PVA), polyvinyl prolidone (PVP) and TiO2 were used to prepare these membranes. Characterization of the modified nanofiber membranes was also performed by using scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), and atomic force microscopy (AFM) techniques. The characterization techniques have proven that the prepared nanofiber membranes are nano-structured. The antibacterial properties of modified nanofiber membranes containing different amounts of AgNO3 were tested against selected E. coli (25922), S. aureus (ATCC 25923, ATCC 6538) and P. aeruginosa (ATCC 35032) bacteria by using two different methods (Agar disc diffusion and Baird Parker agar plate). As a result, the antibacterial activities of modified nanofiber membranes increased against all bacteria with the addition of silver ions.

Anahtar Kelimeler

Antibacterial, Characterization, Membrane, Nanofiber

Proje Numarası

-

Kaynakça

• Abdelgawad, A.M., Hudson, S.M., Rojas, O.J., 2014. Antimicrobial wound dressing nanofiber mats from multicomponent (Chitosan/Silver-Nps/Polyvinyl Alcohol) systems. Carbohydrate Polymers, 100, 166-178.
• Adibzadeh, S., Bazgir, S., Katbab, A.A., 2014. Fabrication and characterization of chitosan/poly(vinyl alcohol) electrospun nanofibrous membranes containing silver nanoparticles for antibacterial water filtration. Iranian Polymer Journal, 23, 645-654.
• Au, H.T., Pham, L.N., Vu, T.H.T., Park, J.S., 2012. Fabrication of an antibacterial non-woven mat of a poly(lactic acid)/chitosan blend by electrospinning. Macromolecular Research, 20, 51-58.
• Baştürk, E., 2012. Çapraz bağlı PVA/B nanofiberlerin elektrospin yöntemi ile hazırlanması ve karakterizasyonu, Yüksek Lisans Tezi, Marmara Üniversitesi Fen Bilimleri Enstitüsü, İstanbul, 132.
• Beypınar, F., 2014. Polietilenimin esaslı nanofiberlerin hazırlanması ve karbon dioksit gazı geçirgenliğinin incelenmesi, Yüksek Lisans Tezi, Marmara Üniversitesi Fen Bilimleri Enstitüsü, İstanbul, 67.
• Biswas, C., Zuo, X., Chen, S.C., Schibeci, S.D., Forwood, J.K., Jolliffe, K.A., Sorrell, T.C., Djordjevic, J.T., 2014. Functional disruption of yeast metacaspase, Mca1, leads to miltefosine resistance and inability to mediate miltefosine-induced apoptotic effects. Fungal Genetics and Biology, 67, 71-81.
• Burger, C., Hsiao, B.S., Chu, B., 2006. Nanofibrous materials and their applications. Annual Review of Materials Research, 36, 333-368.
• Depkes, R.I., 1989. Materia Medika Indonesia, Jilid V. Jakarta: Departemen Kesehatan Republik Indonesia. Halaman, 549-553.
• Fabrega, J., Fawcett, S.R., Renshaw, J.C., Lead, J.R., 2009. Silver nanoparticle impact on bacterial growth: effect of pH, concentration, and organic matter. Environmental Science & Technology, 43, 7285-7290.
• Fujihara, K., Huang, Z.M., Ramakrishna, S., Satknanantham, K., Hamada, H., 2003. Performance study of braided carbon/PEEK composite compression bone plates. Biomaterials, 24(15), 2661-2667.
• Gao, Y., Truong, Y.B., Zhu, Y., Kyratzis, I.L., 2014. Electrospun antibacterial nanofibers: production, activity, and in vivo applications. Journal of Applied Polymer Science, 131(18), 1-13.
• Gülbandılar, A., 2009. Kütahya yöresinde burun mukozasındaki Staphylococcus aureus taşıyıcılığının ve antibiyotik duyarlılığının araştırılması. Dumlupınar Üniversitesi Fen Bilimleri Enstitüsü Dergisi (Journal of Scientific Reports-A), 18, 1-6.
• Halkman, B.D.H., Yücel, K.P., 2005. Gıdalarda Radyasyon Uygulamalarının Mikroorganizmalar Üzerine Etkileri, Gıda, 30(6), 409-416.
• Hang, A.T., Tae, B., Park, J.S., 2010. Non-woven mats of poly(vinyl alcohol)/chitosan blends containing silver nanoparticles: Fabrication and characterization. Carbohydrate Polymers, 82, 472-479.
• Hong, H.F., Jeong, S., 2011. Effect of nano sized silver on electrospun nylon-6-fiber. Journal of Nanoscience and Nanotechnology,11(1), 372-376.
• Huang, Z.M., Zhang, Y.Z., Kotaki, M., Ramakrishna, S., 2003. A review on polymer nanofibers by electro-spinning applications in nanocomposites. Composites Science and Technology, 63, 2223- 2253.
• Ignatova, M., Rashkov, I., Manolova, N., 2013. Drug-loaded electrospun materials in wound-dressing applications and in local cancer treatment. Expert Opinion on Drug Delivery, 10, 469- 483.
• Jia, Y.T., Gong, J., Gu, X.H., Kim, H.Y., Dong, J., Shen, X.Y., 2007. Fabrication and characterization of poly(vinyl alcohol)/chitosan blend nanofibers produced by electrospinning method. Carbohydrate Polymers, 67, 403-409.
• Kwoun, S.J., Lec, R.M., Han, B., Ko, F.K., 2001. Polymer nanofiber thin films for biosensor applications. In: Proceedings of the IEEE 27th Annual Northeast Bioengineering Conference, 9– 10.
• Laurencin, C.T., Ambrosio, A.M.A., Borden, M.D., Cooper Jr, J.A., 1999. Tissue engineering: orthopedic applications. Annual Review of Biomedical Engineering, 1, 19–46.
• Liang, D., Lu, Z., Yang, H., Gao, J., Chen, R., 2016. Novel asymmetric wettable AgNPs/Chitosan wound dressing: In Vitro and In Vivo evaluation. ACS Applied Materials & Interfaces, 8(6), 3958-3968.
• Lim, S.H., Hudson, S.M., 2003. Review of chitosan and its derivatives as antimicrobial agents and their uses as textile chemicals. Journal of Macromolecular Science: Part C: Polymer Reviews, C43, 223-269.
• Lu, P., Ding, B., 2008. Applications of electrospun fibers. Recent Patents on Nanotechnology, 2, 169-182.
• Mahapatra, A., Garg, N., Nayak, B.P., Mishra, B.G., Hota, G., 2012. Studies on the synthesis of electrospun PAN‐Ag composite nanofibers for antibacterial application. Journal of Applied Polymer Science, 124(2), 1178-1185.
• Nickerson, J.T., Sinskey, A.J., 1974. Microbiology of Foods And Food Processing, American Elsevier Publishing Co., Inc. New York, 18(4): 471.
• Ohkawa, K., Cha, D.I., Kim, H., Nishida, A., Yamamoto, H., 2004. Electrospinning of chitosan. Macromolecular Rapid Communications, 25, 1600-1605.
• Omerovic, M., Müştak, H.K., Kaya, İ.B., 2017. Escherichia coli patotiplerinin virülens faktörleri. Etlik Veteriner Mikrobiyoloji Dergisi, 28(1), 1-6.
• Pokhrel, S., Yadav, P.N., Adhikari, R., 2015. Applications of chitin and chitosan in industry and medical science: A Review. Nepal Academy of Science and Technology, 16, 99-104.
• Rinaudo, M., 2006. Chitin and chitosan: Properties and applications. Progress in Polymer Science, 31, 603-632.
• Sırıken, B., Öz, V., 2017. Pseudomonas aeruginosa: özellikleri ve quorum sensing mekanizması. Gıda ve Yem Bilimi Teknolojisi Dergisi, 18, 42-52.
• Teo, W.E., Ramakrishna, S., 2006. A Review on electrospinning design and nanofibre assemblies. Nanotechnology, 17, R89-R106.
• Tian, L., Wang, P., Zhao, Z., Ji, J., 2013. Antimicrobial activity of electrospun poly(butylenes succinate) fiber mats containing PVP-capped silver nanoparticles. Applied Biochemistry and Biotechnology, 171(7), 1890-1899.
• Tucker, N., Stanger, J.J., Staiger, M.P., Razzaq, H., Hofman, K., 2012. The history of the science and technology of electrospinning from 1600 to 1995. Journal of Engineered Fibers and Fabrics, 7, 63-73.
• Xu, H., Ma, L., Shi, H., Gao, C., Han, C., 2007. Chitosan-hyaluronic acid hybrid film as a novel wound dressing: in vitro and in vivo studies. Polymers for Advanced Technologies, 18, 869-875.
• Yuan, J., Geng, J., Xing, Z., Shen, J., Kang, I.K., Byun, H., 2010. Electrospinning of antibacterial poly(vinylidene fluoride) nanofibers containing silver nanoparticles. Journal of Applied Polymer Science, 116(2), 668-672.
• Zahedi, P., Rezaeian, I., Ranaei-Siadat S.O., Jafari, S.H., Supaphol, P., 2010. A Review on wound dressings with an emphasis on electrospun nanofibrous polymeric bandages. Polymers for Advanced Technologies, 21, 77- 95.
• Zeytuncu, B., 2014. Elektrospinning tekniği ve UV ışımasının eşzamanlı olarak uygulanması ile nanofiber membranların hazırlanması ve kıymetli metallerin adsorpsiyonunda uygulanması. Doktora Tezi, İstanbul Teknik Üniversitesi Fen Bilimleri Enstitüsü, İstanbul, 172.
• Zhang, Y.Z., Lim, C.T., Ramakrishna, S., Huang, Z.M., 2005. Recent development of polymer nanofibers for biomedical and biotechnological applications. Journal of Materials Science: Materials in Medicine, 16, 933-946.
• Zhang, Y., Huang, X., Duan, B., Wu, L., Li, S., Yuan, X., 2007. Preparation of electrospun chitosan/poly(vinyl alcohol) membranes. Colloid and Polymer Science, 285, 855-863.
• Zhang, W., Xing, M.M.Q., Maibach, H.I., 2010. Nanofibrous materials for wound care. Cutaneous and Ocular Toxicology, 29, 143-152.
• Zhang, L., Bai, X., Tian, H., Zhong, L., Ma, C., Zhou, Y., Chen, S., Li, D., 2012. Synthesis of antibacterial film CTS/PVP/TiO2/Ag for drinking water system. Carbohydrate Polymers, 89, 1060-1066.