Melatonin Extraction and Determination by UHPLC-FD from Different Plants
Abstract
Many plants have become a piece of the traditional food as human diet and evaluated in folk medicine to treat diseases. They are very rich in terms of nutraceuticals. Melatonin functions as a free radical scavenger and controls the regulation of the sleep–wake cycle in mammals. In this study, we analyzed melatonin content in the herba parts of Stachys lavandulifolia, Tribulus terrestris, and Helichrysum arenarium, and unripe fruits of Prunus armeniaca L., Prunus cerasifera, and Prunus amygdalus L. A solvent mixture of methanol:ultrapure water:HCl (70:29.9:01 v/v/v) was used for the extraction of melatonin from plant samples. The determination of melatonin in the extracts was carried out by high pressure liquid chromatography with fluorescence detector (UHPLC-FD). Melatonin concentration was 0.0231 mg/kg for herba of Helichrysum arenarium and 0.0018 mg/kg for Prunus armeniaca L. Melatonin was not detected in other plant samples.
Keywords
References
- Verde A, Miguez JM, Leao-Martins JM, Gago-Martinez A, Gallardo M. Melatonin Content in Walnuts and Other Commercial Nuts. Influence of Cultivar, Ripening and Processing (roasting). Journal of Food Composition and Analysis, 2022; 104180.
- Arnao MB, Ruiz JH. Melatonin in plants. Plant Signaling & Behavior, 2007; 2: 381-382.
- Fracassetti D, Vigentini I, Lo Faro AFF, De Nisi P, Foschino R, Tirelli A, Orioli M, Iriti M. Assessment of Tryptophan, Tryptophan Ethylester, and Melatonin Derivatives in Red Wine by SPE-HPLC-FL and SPE-HPLC-MS Methods. Food, 2019; 8: 99.
- Varga-Visi E, Jocsak I, Loki Katalin, Vegvari K. Analysis of melatonin and serotonin from cherries: a review. Acta Agraria Kaposvariensis, 2018; 22: 52-60.
- Pranil T, Moongngarm A, Manwiwattanakul G, Loypimai PL, Kerr W. Melatonin and its derivative contents in tropical fruits and fruit tablets. Journal of Food Composition and Analysis, 2021; 103: 104109.
- Escrivá L, Manyes L, Barberà M, Martínez-Torres D, Meca G. Determination of melatonin in Acyrthosiphon pisum aphids by liquid chromatography-tandem mass spectrometry. Journal of Insect Physiology, 2016; 86: 48–53.
- Meng X, Li Y, Li S, Zhou Y, Gan RY, Xu DP, Li HB. Dietary sources and bioactivities of melatonin. Nutrients, 2017; 9: 367.
- Zia-Ul-Haq M, Ahmad S, Qayum M, Ercisli S. Compositional studies and antioxidant potential of Albizia lebbeck (L.) Benth. Pods and seeds. Turkish Journal of Biology. 2013; 37: 25-32.
- King, F.B. Interpreting wild food plants in archaeological record. In: Etkin NL, editor. Eating on the wild side. Tucson: University of Arizona Press; 1994.
- Hernández-Ruiz J, Arnao MB. Distribution of melatonin in diferent zones of lupin and barley plants at diferent ages in the presence and absence of light. Journal of Agricultural and Food Chemistry, 2008; 56:10567–10573.