Comparison of Serum Albumin Separation by Ammonium Sulfate Precipitation and Cibacron Blue Affinity Chromatography

Esmanur Çiğ; Tayfun Güldür

doi:10.71133/anatphar.1686487

Comparison of Serum Albumin Separation by Ammonium Sulfate Precipitation and Cibacron Blue Affinity Chromatography

Abstract

Serum albumin, which is synthesized in the liver and has many physiological functions, is one of the most abundant proteins in plasma. Albumin is a valuable biomarker of many diseases and has indications in many diseases. The increasing use of albumin in biotechnological applications, its important ligand-carrying properties and its wide range of applications in clinical and basic scientific studies require high purity serum albumin separation. Many methods such as ammonium sulfate precipitation, heat shock method, plasma fractionation with ethanol, chromatographic methods (column, affinity), acetone/trichloroacetic acid precipitation are used for albumin separation from serum. In this study, 2 methods based on ammonium sulfate precipitation and Cibacron Blue affinity chromatography were compared. The purity of the isolated proteins was monitored by SDS-PAGE and densitometrically analyzed. The selected methods were evaluated in terms of time, applicability, purity and cost. İt was concluded that ammonium sulfate precipitation provided a more successful separation in terms of albumin yield and purity compared to Cibacron Blue affinity chromatography.

Keywords

: Serum Albumin , Ammonium Sulfate , Cibacron Blue F3GA , Affinity Chromatography , Protein Fractionation

References

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Details

Primary Language

English

Subjects

Biochemistry and Cell Biology (Other)

Journal Section

Research Article

Authors

Esmanur Çiğ ^*
0000-0002-7388-0817
Türkiye

Tayfun Güldür
0000-0002-1623-2880
Türkiye

Publication Date

September 3, 2025

Submission Date

April 30, 2025

Acceptance Date

May 26, 2025

Published in Issue

Year 2025 Volume: 4 Number: 2

DOI

https://doi.org/10.71133/anatphar.1686487

IZ

https://izlik.org/JA64WW33WR

EndNote

Çiğ E, Güldür T (September 1, 2025) Comparison of Serum Albumin Separation by Ammonium Sulfate Precipitation and Cibacron Blue Affinity Chromatography. Anatolian Journal of Pharmaceutical Sciences 4 2 63–75.

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Comparison of Serum Albumin Separation by Ammonium Sulfate Precipitation and Cibacron Blue Affinity Chromatography

Abstract

Keywords

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

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