The relationship between hydrogen peroxide and polyamine metabolism and the role of NADPH oxidase (NOX) in this relationship under drought conditions has not yet been clarified. To reveal the relationship, expression levels of the genes in polyamine metabolism, such as arginine decarboxylase (ADC), agmatine aminohydrolase (AIH), spermidine synthase (SPDS), S-adenosyl methionine decarboxylase (SAMDC), diamine oxidase (DAO) and polyamine oxidase (PAO) were determined by RT PCR under drought stress combined with exogenous hydrogen peroxide (H2O2) and diphenyleneiodonium chloride (DPI) treatments in maize seedlings. Also, some basic stress parameters (leaf water potential, lipid peroxidation), polyamine levels (putrescine, spermidine, and spermine), and gene expression of NOX were measured under drought stress. Exogenous H2O2 induced polyamine contents by up-regulating polyamine-synthesizing genes and downregulating polyamine oxidizing genes. Moreover, when the NOX enzyme was inhibited by DPI, the polyamine metabolic pathway tended toward degradation rather than production. Exogenous H2O2 regulated polyamine metabolism to promote their synthesis, and NOX had a key role in this regulation.
Diphenyleneiodonium chloride, Drought stress, Hydrogen peroxide, NAPH oxidase, Polyamine metabolism
Birincil Dil | İngilizce |
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Konular | Fen |
Bölüm | Makaleler |
Yazarlar |
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Destekleyen Kurum | The Scientific and Technological Research Council of Turkey |
Proje Numarası | 113Z863 |
Teşekkür | Authors thanks to Prof. Dr. Ahmet YASAR for helping measurements of polyamine contents. |
Yayımlanma Tarihi | 4 Aralık 2022 |
Başvuru Tarihi | 14 Ocak 2021 |
Kabul Tarihi | 8 Kasım 2021 |
Yayınlandığı Sayı | Yıl 2022, Cilt Early View, Sayı |
Bibtex | @araştırma makalesi { ankutbd861008, journal = {Journal of Agricultural Sciences}, eissn = {2148-9297}, address = {}, publisher = {Ankara Üniversitesi}, year = {2022}, volume = {Early View}, pages = {40 - 40}, doi = {10.15832/ankutbd.861008}, title = {Investigation of The Roles of Hydrogen Peroxide and NADPH Oxidase in The Regulation of Polyamine Metabolism in Maize Plants under Drought Stress Conditions}, key = {cite}, author = {Demiralay, Mehmet and Sağlam, Aykut and Yetişsin, Fuat and Kadıoğlu, Asım} } |
APA | Demiralay, M. , Sağlam, A. , Yetişsin, F. & Kadıoğlu, A. (2022). Investigation of The Roles of Hydrogen Peroxide and NADPH Oxidase in The Regulation of Polyamine Metabolism in Maize Plants under Drought Stress Conditions . Journal of Agricultural Sciences , Early View , 40-40 . DOI: 10.15832/ankutbd.861008 |
MLA | Demiralay, M. , Sağlam, A. , Yetişsin, F. , Kadıoğlu, A. "Investigation of The Roles of Hydrogen Peroxide and NADPH Oxidase in The Regulation of Polyamine Metabolism in Maize Plants under Drought Stress Conditions" . Journal of Agricultural Sciences Early View (2022 ): 40-40 <https://dergipark.org.tr/tr/pub/ankutbd/issue/68522/861008> |
Chicago | Demiralay, M. , Sağlam, A. , Yetişsin, F. , Kadıoğlu, A. "Investigation of The Roles of Hydrogen Peroxide and NADPH Oxidase in The Regulation of Polyamine Metabolism in Maize Plants under Drought Stress Conditions". Journal of Agricultural Sciences Early View (2022 ): 40-40 |
RIS | TY - JOUR T1 - Investigation of The Roles of Hydrogen Peroxide and NADPH Oxidase in The Regulation of Polyamine Metabolism in Maize Plants under Drought Stress Conditions AU - Mehmet Demiralay , Aykut Sağlam , Fuat Yetişsin , Asım Kadıoğlu Y1 - 2022 PY - 2022 N1 - doi: 10.15832/ankutbd.861008 DO - 10.15832/ankutbd.861008 T2 - Journal of Agricultural Sciences JF - Journal JO - JOR SP - 40 EP - 40 VL - Early View IS - SN - -2148-9297 M3 - doi: 10.15832/ankutbd.861008 UR - https://doi.org/10.15832/ankutbd.861008 Y2 - 2021 ER - |
EndNote | %0 Tarım Bilimleri Dergisi Investigation of The Roles of Hydrogen Peroxide and NADPH Oxidase in The Regulation of Polyamine Metabolism in Maize Plants under Drought Stress Conditions %A Mehmet Demiralay , Aykut Sağlam , Fuat Yetişsin , Asım Kadıoğlu %T Investigation of The Roles of Hydrogen Peroxide and NADPH Oxidase in The Regulation of Polyamine Metabolism in Maize Plants under Drought Stress Conditions %D 2022 %J Journal of Agricultural Sciences %P -2148-9297 %V Early View %N %R doi: 10.15832/ankutbd.861008 %U 10.15832/ankutbd.861008 |
ISNAD | Demiralay, Mehmet , Sağlam, Aykut , Yetişsin, Fuat , Kadıoğlu, Asım . "Investigation of The Roles of Hydrogen Peroxide and NADPH Oxidase in The Regulation of Polyamine Metabolism in Maize Plants under Drought Stress Conditions". Journal of Agricultural Sciences Early View / (Aralık 2022): 40-40 . https://doi.org/10.15832/ankutbd.861008 |
AMA | Demiralay M. , Sağlam A. , Yetişsin F. , Kadıoğlu A. Investigation of The Roles of Hydrogen Peroxide and NADPH Oxidase in The Regulation of Polyamine Metabolism in Maize Plants under Drought Stress Conditions. J Agr Sci-Tarim Bili. 2022; Early View: 40-40. |
Vancouver | Demiralay M. , Sağlam A. , Yetişsin F. , Kadıoğlu A. Investigation of The Roles of Hydrogen Peroxide and NADPH Oxidase in The Regulation of Polyamine Metabolism in Maize Plants under Drought Stress Conditions. Journal of Agricultural Sciences. 2022; Early View: 40-40. |
IEEE | M. Demiralay , A. Sağlam , F. Yetişsin ve A. Kadıoğlu , "Investigation of The Roles of Hydrogen Peroxide and NADPH Oxidase in The Regulation of Polyamine Metabolism in Maize Plants under Drought Stress Conditions", Journal of Agricultural Sciences, c. Early View, ss. 40-40, Ara. 2022, doi:10.15832/ankutbd.861008 |