Revealing Mucilage Event-Linked Community Composition in the Sea of Marmara from eDNA Metabarcoding Data

Yıl 2024, , 106 - 120, 29.03.2024

Onur Doğan , Anıl Doğan Örün , Raşit Bilgin , Melek İşinibilir

https://doi.org/10.26650/ASE20241434217

Öz

Mucilage events are among the most conspicuous phenomena in marine ecosystems and present numerous challenges in determining the composition of communities associated with them. To overcome this problem, we used environmental DNA (eDNA) metabarcoding approaches to reveal the species-level resolution of community composition. Mucilaginous aggregates were sampled at six collecting sites during a novel mucilage event (autumn 2021–summer 2022) in the Sea of Marmara, Türkiye. A wide range of plankton community compositions was detected in mucilage samples. eDNA metabarcoding was effective in predicting the community composition of mucilage, which is composed of a wide variety of organisms from mucilaginous aggregates.

Anahtar Kelimeler

eDNA, metabarcoding, mucilage, the Sea of Marmara, molecular ecology, biomonitoring

Etik Beyan

No animal testing was performed during this study.

Destekleyen Kurum

Scientific Research Projects Coordination Unit of Istanbul University, and Scientific and Technological Research Council of Türkiye (TÜBITAK).

Proje Numarası

36858, and 121G096.

Teşekkür

We are thankful to the crew of the Yunus-S R/V from the Istanbul University, Faculty of Aquatic Sciences for their help during expedition. The numerical calculations reported in this paper were partially performed at the TUBITAK ULAKBIM, High Performance and Grid Computing Center (TRUBA resources).

Kaynakça

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