Oreochromis niloticus’TA CIVANIN BİYOKİMYASAL TOKSİSİTESİ VE BU TOKSİSİTE ÜZERİNE ZEOLİTİN KORUYUCU ETKİSİ

Öz

Bu çalışmada Nil Tilapia’sı Oreochromis niloticus’ta serum enzim aktiviteleri kullanılarak cıva (Hg) toksisitesi ve zeolitin koruyucu etkisi araştırılmıştır. Bu amaçla balıklar 0,01 ve 0,05 mg/L Hg ile 0,01 mg/L Hg+0,01 g/L Zeolit ve 0,05 mg/L Hg+0,05 g/L Zeolit karışımlarının etkisine 4 ve 21 günlük sürelerle bırakılmış ve kan serumunda alanin aminotransferaz (ALT), aspartat aminotransferaz (AST), alkalen fosfataz (ALP) ve laktat dehidrojenaz (LDH) enzim aktiviteleri ölçülmüştür. Doğrudan cıva ve cıva+zeolit karışımlarının etkisinde incelenen serum enzim aktivitelerinde ortam derişimine ve etki süresine bağlı olarak önemli değişiklikler saptanmıştır. ALT, AST ve ALP aktivitelerinde cıvanın her iki ortam derişiminin etkisinde 4 ve 21 günlük süreler; cıva+zeolit karışımının ise yüksek ortam derişiminde ve 21 günlük etki süresi sonunda anlamlı bir artış belirlenmiştir (P˂0,05). LDH aktivitesi ise cıvanın tek başına ve zeolitle birlikte etkisinde her iki ortam derişimlerinde hem 4 hem de 21 günlük süreler sonunda önemli bir artış göstermiştir (P<0,05). Sunulan araştırmada O. niloticus’un serum enzim aktivitelerindeki artışların cıva+zeolit karışımına oranla cıvanın tek başına etkisinde daha fazla olduğu belirlenmiştir. Bu sonuçlar ortamda zeolit bulunduğunda cıva toksisitesinin kısmen ya da tamamen düzeldiğini göstermektedir.

Anahtar Kelimeler

• Oreochromis niloticus,Cıva,Zeolit,Zeolit Serum enzimleri

BIOCHEMICAL TOXICITY OF MERCURY AND PROTECTIVE EFFECT OF ZEOLITE ON THIS TOXICITY IN Oreochromis niloticus

Abstract

In this study, it was investigated mercury (Hg) toxicity and protective effect of zeolite by using serum enzymes activities of Oreochromis niloticus. For this purpose fish were exposed to 0.01 and 0.05 mg/L Hg and 0.01 mg/L Hg+0.01 g/L Zeolite and 0.05 mg/L Hg+0.05 g/L Zeolite for 4 and 21 days and alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) activities were measured in blood serum. In the exposures of mercury alone and mercury+zeolite mixtures, significant changes were determined in all enzymes activities due to medium concentration and exposure period. ALT, AST, and ALP activities significantly increased at 4 and 21 days in both exposure concentrations of mercury and at 21 days in higher concentration of mercury+zeolite mixture (P˂0.05). LDH activity elevated all tested concentrations of mercury and mercury+zeolite mixtures after the both exposure periods (P˂0.05). In the present research it was determined increases in activities of serum enzymes of O. niloticus were higher in the mercury alone than mercury+zeolite mixture and zeolite partially or totally played a protective role aganist the toxic effect of Hg. 

Keywords

• Oreochromis niloticus,Mercury,Zeolite,Serum enzymes

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