Oreochromis niloticus’TA CIVANIN BİYOKİMYASAL TOKSİSİTESİ VE BU TOKSİSİTE ÜZERİNE ZEOLİTİN KORUYUCU ETKİSİ

Year 2016, Volume: 31 Issue: 2, 86 - 95, 03.12.2016

Özgür Fırat , Arzu Şahin İnandı

Abstract

Bu çalışmada Nil Tilapia’sı Oreochromis niloticus’ta serum enzim aktiviteleri kullanılarak cıva
(Hg) toksisitesi ve zeolitin koruyucu etkisi araştırılmıştır. Bu amaçla
balıklar 0,01 ve 0,05 mg/L Hg ile 0,01 mg/L Hg+0,01 g/L Zeolit ve 0,05 mg/L
Hg+0,05 g/L Zeolit karışımlarının etkisine 4 ve 21 günlük sürelerle bırakılmış
ve kan serumunda alanin aminotransferaz (ALT), aspartat aminotransferaz (AST),
alkalen fosfataz (ALP) ve laktat dehidrojenaz (LDH) enzim aktiviteleri
ölçülmüştür. Doğrudan cıva ve cıva+zeolit karışımlarının etkisinde incelenen
serum enzim aktivitelerinde ortam derişimine ve etki süresine bağlı olarak
önemli değişiklikler saptanmıştır. ALT, AST ve ALP aktivitelerinde cıvanın her
iki ortam derişiminin etkisinde 4 ve 21 günlük süreler; cıva+zeolit karışımının
ise yüksek ortam derişiminde ve 21 günlük etki süresi sonunda anlamlı bir artış
belirlenmiştir (P˂0,05). LDH aktivitesi ise cıvanın tek başına ve zeolitle
birlikte etkisinde her iki ortam derişimlerinde hem 4 hem de 21 günlük süreler
sonunda önemli bir artış göstermiştir (P<0,05). Sunulan araştırmada O. niloticus’un serum enzim
aktivitelerindeki artışların cıva+zeolit karışımına oranla cıvanın tek başına
etkisinde daha fazla olduğu belirlenmiştir. Bu sonuçlar ortamda zeolit
bulunduğunda cıva toksisitesinin kısmen ya da tamamen düzeldiğini göstermektedir.

Keywords

Oreochromis niloticus, Cıva, Zeolit, Zeolit Serum enzimleri

BIOCHEMICAL TOXICITY OF MERCURY AND PROTECTIVE EFFECT OF ZEOLITE ON THIS TOXICITY IN Oreochromis niloticus

Abstract

In this study, it was investigated mercury (Hg)
toxicity and protective effect of zeolite by using serum enzymes activities of Oreochromis niloticus. For this purpose
fish were exposed to 0.01 and 0.05 mg/L Hg and 0.01 mg/L Hg+0.01 g/L Zeolite
and 0.05 mg/L Hg+0.05 g/L Zeolite for 4 and 21 days and alanine
aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase
(ALP), and lactate dehydrogenase (LDH) activities were measured in blood serum.
In the
exposures of mercury alone and mercury+zeolite mixtures, significant changes
were determined in all enzymes activities due to medium concentration and
exposure period. ALT, AST, and ALP activities significantly increased at 4 and
21 days in both exposure concentrations of mercury and at 21 days in higher
concentration of mercury+zeolite mixture (P˂0.05). LDH activity elevated all tested concentrations of mercury and
mercury+zeolite mixtures after the both exposure periods (P˂0.05). In the
present research it was determined increases in activities of serum enzymes of O. niloticus were higher in the mercury
alone than mercury+zeolite mixture and zeolite partially or totally played a protective role aganist the toxic effect of Hg. 

Keywords

Oreochromis niloticus, Mercury, Zeolite, Serum enzymes

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