Yetişkin Sıçanlarda Testis Hücreleri ve Kalsinörin Immunopozitifliği Üzerine Ketaminin Etkileri: Bir Immunohistokimyasal ve Histopatolojik Çalışma

Yıl 2012, Cilt: 7 Sayı: 1, 7 - 17, 25.04.2012

Yıldıray Kalkan , Levent Tümkaya Habib Bostan Yakup Tomak Durdu Altuner Orhan Baş Orhan Ünal Zorba Hakkı Uzun

Öz

Bu çalışmada, sıçanlara uygulanan uzun süreli ketaminin testis germ hücreleri ve kalsinörin immunopozitif hücreler
üzerine etkilerinin araştırılması amaçlandı. Otuz adet, yetişkin Sprague-Dawley erkek sıçan her grupta 6’şar adet olacak
şekilde 5 gruba ayrıldı. On dört gün süreyle ve günde iki defa olmak üzere, grup 1, 2, 3 ve 4’e sırasıyla 40, 60, 80 ve 100
mg/kg ketamin hidroklorid ve grup 5’e ise sadece % 0,9 NaCl intraperitonal (i.p.) olarak uygulandı. Bu çalışmada, ketamin
uygulanan gruplarda testis bağ dokusunda ödem ve germ hücrelerinde dejenerasyonlar gözlendi. Grup 3 ve 4’te, tubulus
seminiferus lumenlerinde sperm miktarındaki azalmanın grup 1 ve 2’ye göre daha fazla olduğu belirlendi. Uzun süreli ve
yüksek doz ketamin kullanımının bir sonucu olarak, spermatogenetik germ hücreleri ve Leydig hücreleri miktarında da
azalma olduğu tespit edildi. Ayrıca, immunhistokimyasal yöntemler ile boyanmış testis dokusu kesitlerinde, kalsinörin’in
değişik derecelerde, (+)’dan (+++)’a kadar, immunoreaktivite verdiği gözlendi. Sonuç olarak, uzun süreli ve yüksek doz
ketamin kullanımının testis intersitisyel dokularında, sperm yoğunluğunda ve germ hücre miktarlarında histopatolojik ve
immunohistokimyasal değişiklikler yapabileceği belirlenmiş oldu. Ayrıca, uzun süreli ketamin infüzyonunun erkek
infertilitesine neden olabileceği de düşünülmektedir.

Anahtar Kelimeler

İmmunohistokimya, Kalsinörin, Ketamin, Sıçan, Testis

Kaynakça

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