Anti-Aging Effects of Hibiscus sabdariffa on Immortalized Human Ovarian Epithelial Cells
Abstract
Objectives: The aim of this study is to examine the anti-aging effect of Hibiscus sabdariffa (HS) against oxidative stress induced by hydrogen
peroxide (H2O2) on immortalized human ovarian epithelial cells (IHOECs).
Materials and Methods: The obtained IHOECs were combined with 12.5 mg/mL HS extracted by adding 1mL of distilled water (dH2O) and different
concentrations of H2O2 for oxidative effect. IHOECs cell viability was increased with HS extract and determined by MTT test assay. Real-time
polymerase chain reaction was conducted to amplify and detect apoptosis-related BAX and BCL2, stem cell markers SOX2 and OCT4, and antioxidative
nuclear factor erythroid 2-related factor 2 (NRF2) gene expressions. For the statistical analysis Kruskal-Wallis analysis and Mann-Whitney U test
were used in SPSS program.
Results: The increase in HS in cell proliferation was 14%. While there is no significant increase of cell viability in the combined group with HS and
H2O2, there were only statistically significant groups are 200, 800, 900 and, 1,000 μl. It was indicated that HS has apoptotic, stem cell promotion and
antioxidative effects on high H2O2 concentrations of these groups (p<0.05).
Conclusion: In combined groups with both HS and H2O2 induced oxidative stress, HS increased the expression of proapoptotic gene BAX and
decreased antiapoptotic gene BCL2. Although, the remaining cells which are abundantly stem cells expressed genes OCT4 and SOX2 remarkably
decreased, at high H2O2 concentration opposite effects were observed. NRF2 gene expression also increased in this high oxidative stress.
Keywords
Hibiscus sabdariffa, oxidative stress, apoptosis, stem cells
Ethical Statement
The cells used in the study are commercially available so ethics committee approval is not required. The study protocol was appropriate in accordance with the Declaration of Helsinki.
References
- 1. Noll C, Raaf L, Planque C, et al. Protection and reversal of hepatic fibrosis by red wine polyphenols in hyperhomocysteinemic mice. J Nutr Biochem. 2011;22:856-864.
- 2. Zhao W, Ji X, Zhang F, et al. Embryonic stem cell markers. Molecules. 2012;17:6196-6236.
- 3. Sporn MB, Liby KT. NRF2 and cancer: the good, the bad and the importance of context. Nat Rev Cancer. 2012;12:564-571.
- 4. da Silva PFL, Schumacher B. Principles of the Molecular and Cellular Mechanisms of Aging. J Invest Dermatol. 2021;141:951-960.
- 5. Wang D, Nagata M, Matsumoto M, et al. Potential of Hibiscus sabdariffa L. and Hibiscus Acid to Reverse Skin Aging. Molecules. 2022;27:6076.
- 6. Alkadi H. A Review on Free Radicals and Antioxidants. Infect Disord Drug Targets. 2020;20:16-26.
- 7. Riaz G, Chopra R. A review on phytochemistry and therapeutic uses of Hibiscus sabdariffa L. Biomed Pharmacother. 2018;102:575-586.
- 8. Ighodaro O. M., Akinloye O. A. First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid. Alexandria Journal of Medicine. 2018;54:287-293.
- 9. Schuyer M, van der Burg ME, Henzen-Logmans SC, et al. Reduced expression of BAX is associated with poor prognosis in patients with epithelial ovarian cancer: a multifactorial analysis of TP53, p21, BAX and BCL-2. Br J Cancer. 2001;85:1359-67.
- 10. Arnold K, Sarkar A, Yram MA, et al. Sox2(+) adult stem and progenitor cells are important for tissue regeneration and survival of mice. Cell Stem Cell. 2011;9:317-29.