İnsan Pankreas Hücrelerinde Teikoplanin Aktif Madde İçeren Antibiyotiğe Bağlı Sitotoksik Etkilerin MTT Testi ile Değerlendirilmesi

Abstract

Objectives: Antibiotics are a group of drugs used in the treatment of infectious diseases caused by bacteria. They provide powerful treatment against infections, but they are not without side effects. These side effects are not always clinically detectable and their damage at the cellular level may contribute to the development of new disorders. Teicoplanin is one of the glycopeptide antibiotics and is used as a potent agent in the treatment of serious infections caused by Gram-positive bacteria. It may have side effects at cellular level. Materials and Methods: In this study, the cytotoxicity of the antibiotic teicoplanin in human pancreatic cell (hTERT-HPNE) was investigated. Cytotoxicity was determined using the MTT test. hTERT-HPNE cells were exposed to drug containing the active ingredient teicoplanin. The experimental group concentration was prepared in the range of 0.054 mM, 0.109 mM, 0.218 mM, 0.437 mM, 0.875 mM, 1.75 mM, 3.5 mM, 7 mM. Results: The active ingredient teicoplanin caused a change in the morphology of the cells in the MTT test. Dose-related decreases in cell viability were observed with the effect of teicoplanin and they were found to be statistically significant compared to the negative control (p<0.05). As a result of the experiment, it was determined that teicoplanin caused cytotoxicity on hTERT-HPNE. Conclusion: Our study supports the hypothesis that teicoplanin-based antibiotic may have cytotoxic effects on human pancreatic cells and draws attention to the side effects of antibiotics at the cellular level. Key Words: Teicoplanin, Glycopeptide Antibiotic, MTT Test, Human Pancreatic Cell

Keywords

• Teicoplanin
• Glycopeptide Antibiotic
• MTT Test
• Human Pancreatic Cell

Abstract

Amaç: Antibiyotikler, bakterilerin neden olduğu bulaşıcı hastalıkların tedavisinde kullanılan bir grup ilaçtır. Enfeksiyonlara karşı güçlü tedavi sağlarlar, ancak yan etkisiz değillerdir. Bu yan etkiler her zaman klinik olarak saptanmayabilir ve hücresel düzeyde gelişebilir. Hücresel düzeydeki hasarlar yeni bozukluklara yolaçabilir. Teikoplanin, glikopeptid antibiyotiklerden biridir ve Gram-pozitif bakterilerin neden olduğu ciddi enfeksiyonların tedavisinde güçlü bir ajan olarak kullanılır. Hücresel düzeyde yan etkileri olabilir. Gereç ve Yöntem: Bu çalışmada, teikoplanin antibiyotiğinin insan pankreas hücresinde (hTERT-HPNE) sitotoksisitesi araştırıldı. Sitotoksisite MTT testi kullanılarak belirlendi. hTERT-HPNE hücreleri, teikoplanin etken maddesi içeren ilaca maruz bırakıldı. Deney grubu konsantrasyonu, 0,054 mM, 0,109 mM, 0,218 mM, 0,437 mM, 0,875 mM, 1,75 mM, 3,5 mM, 7 mM aralığında hazırlandı. Bulgular: Teikoplanin, MTT testinde hücrelerin morfolojisinde değişikliğe neden oldu. Teikoplanin dozuna bağlı olarak hücre canlılığında azalmalar gözlendi ve negatif kontrole kıyasla istatistiksel olarak anlamlı bulundu (p<0,05). Deney sonucunda teikoplaninin hTERT-HPNE üzerinde sitotoksisiteye neden olduğu belirlendi. Sonuç: Çalışmamız teikoplanin bazlı antibiyotiğin insan pankreas hücreleri üzerinde sitotoksik etkileri olabileceği hipotezini desteklemekte ve antibiyotiklerin hücresel düzeyde yan etkilerine dikkat çekmektedir. Anahtar Kelimeler: Teikoplanin, Glikopeptid Antibiyotik, MTT Testi, İnsan Pankreas Hücresi

Keywords

• Teikoplanin
• Glikopeptid Antibiyotik
• MTT Testi
• İnsan Pankreas Hücresi

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