Baicalein, H2 O2 Kaynaklı L929 Hücre Hasarında Koruyucu Etkiye Sahiptir
Abstract
Objectives: In this study, it was aimed to investigate the possible protective effects of baicalein in H2O2-induced L929 fibroblast cell model by determining the parameters of oxidative stress (SOD) and inflammation process (TNF-alpha, TGF-beta, IL-10).
Materials and Methods: Baicalein was applied at different doses (200, 100 and 80, μM) to L929 fibroblast cells. After 23 hours of incubation, 1 mM
H2O2 was added and cell viability was test by CVDK8. Then, fluorescent diacetate (FDA)-propidium iodide (PI) staining was performed to determine
the ratio of live and dead cells. The levels of TNF-alpha, IL10, TGF-beta and the activity of SOD were determined by ELISA.
Results: After 1 h incubation with 1 mM H2O2, the viability decreased by approximately 77%. It was observed that cell viability increased the most
in the B100 group, with/without H2O2. The cell viability increased after 24 h of baicalein and H2O2 co-incubation. The 100 μM baicalein application
reduced the damage of H2O2 up to 63%. When the levels of TNF-alpha were examined, a significant increase was observed in the H2O2-damage group
compared to control group. Additionally, the levels of IL-10 and TGF-beta increased in H2O2-damage group compared to control group; however, the
levels of those cytokines were decreased in baicalein application groups.
Conclusion: Pretreatment with baicalein (especially 200 μM and 100 μM) results in significant findings including the suppression of inflammatory
cytokines, TNF-alpha, TGF-beta, IL-10 and increase of SOD parameter, SOD, in L929 cells. Taking everything into account, baicalein which is a natural
product may be an alternative therapeutic option for prevention of cellular SOD and inflammatory processes. To better understand the potential
beneficial effects of baicalein, molecular mechanisms and cellular targets should be investigated.
Keywords
Baicalein, Hydrogen Peroxide, Inflammation, Oxidative Stress, L929
Ethical Statement
Ethics Committee Approval: This study is a cell culture study and therefore ethics committee approval was not obtained.
Informed Consent: Cell culture study.
Peer-reviewed: Externally peer-reviewed.
Financial Disclosure: The author declared that she did not receive any financial support during this article
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