Age-related Alterations in Cardiac Function and miRNA’s

Yusuf Olgar; Deniz Billur; Belma Turan

doi:10.4274/atfm.galenos.2020.55707

EN TR

Age-related Alterations in Cardiac Function and miRNA’s

Abstract

Objectives: Since changes in cardiac function are closely related to alterations in cardiac gene-expression, post-gene expression modifications exert an important role in these changes. However, the relationship between cardiac miRNAs and structural changes has not been fully elucidated yet. In this study, we aimed to investigate the relationship between structural changes and miRNA gene expression levels in senescent heart. Materials and Methods: Wistar type male rats were used in this study. Rats were grouped as 3-month-old young-adults and 12-month-old adults. Cardiac tissue sections were examined under a light microscope using hematoxylin and eosin and masson trichrome staining protocol. Isolation of miRNA from cardiac tissue homogenate was performed using commercial kits and expression levels were determined by qt-PCR. Results: Histological findings showed that there was hypertrophy in muscle fibers, and an increasing number of fibroblasts and connective tissue elements, especially in the endomysial area in left ventricular tissue. On the other hand, a marked fibrosis was observed in the endomysium fiber density during aging. Biochemical results showed that miRNA-1 miRNA-133a and miRNA-133b gene expression levels were significantly decreased. Conclusion: It suggests that the decrease in miRNA gene expression levels with aging mediates structural and functional changes. These results are important in terms of achieving new treatment-diagnostic goals related to miRNAs to understand the etiology of aging. Key Words: Cardiac Function, miRNA, Histology, Light Microscopy, Qt-PCR, Aging

Keywords

Cardiac Function, miRNA, Histology, Light Microscopy, Qt-PCR, Aging

Yaşa Bağlı Kalp Fonksiyon Değişiklikleri ve miRNA’lar

Abstract

Amaç: Kalp fonksiyonundaki değişimler ile gen ifadelerindeki değişimler arasında yakın ilişkiler olduğu ve özellikle gen ifadesi sonrası modifikasyonların bu değişimlerde başta rol aldığı bilinmektedir. Ancak kalpteki mikroRNA’ların (miRNA) yapısal değişimlerle ilişkisi henüz tam olarak aydınlatılamamıştır. Bu çalışmada yaşa bağlı seyreden yapısal değişimlerin miRNA gen ifade düzeyleriyle olan ilişkisinin araştırılması hedeflenmiştir. Gereç ve Yöntem: Bu çalışmada Wistar türü erkek sıçanlar kullanılmıştır. Sıçanlar 3 aylık genç-yetişkin ve 12 aylık ileri-yaş-yetişkin olarak gruplandırılmıştır. Kalp doku kesitleri hematoksilin ve eozin ve masson trikrom boyama protokolü uygulanarak ışık mikroskobunda incelenmiştir. Kalp dokusu homojenatından miRNA izolasyonu hazır kitler kullanılarak yapılmış, ifade düzeyleri ise qt-PCR ile ölçülmüştür. Bulgular: Histolojik bulgular kalbin sol ventrikül kısmında kalp kası liflerinde hipertrofi ve özellikle endomisyal alanda artan sayıda fibroblastlar ve bağ dokusu elemanlarının olduğunu göstermiştir. Öte yandan yaşlanmayla birlikte endomisyum kılıfı lif yoğunluğunda belirgin bir fibroz yapılanması gözlenmiştir. Biyokimyasal sonuçlar miRNA-1 miRNA-133a ve miRNA-133b gen ifade düzeylerinin azaldığını göstermektedir. Sonuç: Çalışma sonuçları, sıçan kalp dokusunda yaşa paralel olarak miRNA gen ifade düzeylerindeki azalmanın yapısal ve fonksiyonel değişimlere aracılık ettiğini düşündürmektedir. Bu sonuçlar, yaşlanmanın etiyolojisini anlamak için miRNA’larla ilgili yeni tanı-tedavi hedeflerinin gerçekleştirilmesi açısından önemli olduğunu işaret etmektedir. Anahtar Kelimeler: Kalp Fonksiyonu, miRNA, Histoloji, Işık Mikroskopi, Qt-PCR Analizi, Yaşlanma

Keywords

Kalp Fonksiyonu, miRNA, Histoloji, Işık Mikroskopi, Qt-PCR Analizi, Yaşlanma

Ethical Statement

Etik Kurul Onayı: Ankara Üniversitesi Hayvan Deneyleri Yerel Etik Kurulu’ndan izin alınmıştır (karar no: 2018-18-117). Hasta Onayı: Bu çalışmada bir hayvan deneyi olduğundan,hasta onayı alınmamıştır Hakem Değerlendirmesi: Editörler kurulunun dışından olan kişiler tarafından değerlendirilmiştir. Yazarlık Katkıları Konsept: B.T., Y.O., Dizayn: B.T., Y.O., Veri Toplama veya İşleme: Y.O., D.B., Analiz ve Yorumlama: Y.O., D.B., B.T., Literatür Arama: Y.O., D.B., Yazan: B.T. Çıkar Çatışması: Yazarlar tarafından çıkar çatışması bildirilmemiştir. Finansal Destek: Bu çalışmanın finansal desteği, TÜBİTAK SBAG-216S979 no’lu projeden sağlanmıştır.

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Details

Primary Language

English

Subjects

Human Biophysics

Journal Section

Systematic Reviews and Meta Analysis

Authors

Yusuf Olgar This is me
0000-0002-3226-7450
Türkiye

Deniz Billur This is me
0000-0001-8541-8251
Türkiye

Belma Turan ^*
0000-0003-2583-9294
Türkiye

Publication Date

August 31, 2021

Submission Date

September 15, 2020

Acceptance Date

September 30, 2020

Published in Issue

Year 2021 Volume: 74 Number: 2

DOI

https://doi.org/10.4274/atfm.galenos.2020.55707

IZ

https://izlik.org/JA76UD68ZC

APA

Olgar, Y., Billur, D., & Turan, B. (2021). Age-related Alterations in Cardiac Function and miRNA’s. Ankara Üniversitesi Tıp Fakültesi Mecmuası, 74(2), 239-244. https://doi.org/10.4274/atfm.galenos.2020.55707

AMA

1.Olgar Y, Billur D, Turan B. Age-related Alterations in Cardiac Function and miRNA’s. Ankara Üniversitesi Tıp Fakültesi Mecmuası. 2021;74(2):239-244. doi:10.4274/atfm.galenos.2020.55707

Chicago

Olgar, Yusuf, Deniz Billur, and Belma Turan. 2021. “Age-Related Alterations in Cardiac Function and MiRNA’s”. Ankara Üniversitesi Tıp Fakültesi Mecmuası 74 (2): 239-44. https://doi.org/10.4274/atfm.galenos.2020.55707.

EndNote

Olgar Y, Billur D, Turan B (August 1, 2021) Age-related Alterations in Cardiac Function and miRNA’s. Ankara Üniversitesi Tıp Fakültesi Mecmuası 74 2 239–244.

IEEE

[1]Y. Olgar, D. Billur, and B. Turan, “Age-related Alterations in Cardiac Function and miRNA’s”, Ankara Üniversitesi Tıp Fakültesi Mecmuası, vol. 74, no. 2, pp. 239–244, Aug. 2021, doi: 10.4274/atfm.galenos.2020.55707.

ISNAD

Olgar, Yusuf - Billur, Deniz - Turan, Belma. “Age-Related Alterations in Cardiac Function and MiRNA’s”. Ankara Üniversitesi Tıp Fakültesi Mecmuası 74/2 (August 1, 2021): 239-244. https://doi.org/10.4274/atfm.galenos.2020.55707.

JAMA

1.Olgar Y, Billur D, Turan B. Age-related Alterations in Cardiac Function and miRNA’s. Ankara Üniversitesi Tıp Fakültesi Mecmuası. 2021;74:239–244.

MLA

Olgar, Yusuf, et al. “Age-Related Alterations in Cardiac Function and MiRNA’s”. Ankara Üniversitesi Tıp Fakültesi Mecmuası, vol. 74, no. 2, Aug. 2021, pp. 239-44, doi:10.4274/atfm.galenos.2020.55707.

Vancouver

1.Yusuf Olgar, Deniz Billur, Belma Turan. Age-related Alterations in Cardiac Function and miRNA’s. Ankara Üniversitesi Tıp Fakültesi Mecmuası. 2021 Aug. 1;74(2):239-44. doi:10.4274/atfm.galenos.2020.55707

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Journal of Scientific Technology and Engineering Research

https://doi.org/10.53525/jster.1005934