Genç ve Yaşlı Ratların Kalp Dokularında Klotho, Endotelial Nitrit Oksit Sentetaz ve Katalaz Genlerinin İfade Düzeyleri
Abstract
Objectives: In order to evaluate the role of genes in aging process in the heart of rats, the expression levels of Klotho (KL), Endothelial nitrite oxide
synthase (e-NOS) and Catalase (CAT) genes, which are thought to be important in the metabolism, were investigated in the present study.
Materials and Methods: The expression levels of these genes were compared in heart tissues of 24-month-old and 6-month-old male Wistar rats using real time polymerase chain reaction method.
Results: The mRNA level of e-NOS gene was increased significantly in the left side of the hearts of old rats, it was also increased in the right side of the heart, but it was not statistically significant. The mRNA level of CAT gene was decreased significantly in only the left side of the old rat heart compared to that of the young rat. Furthermore, our data on mRNA level of CAT gene determined in the right part of the heart were increased in that of old rats compared to those of the young rats. However, the expression level of KL gene was not significantly different in the left part of the hearts in either old rats or young rats, whereas the expression level of this gene was increased significantly in the right side of the old rat heart compared to that of young rat heart.
Conclusion: KL, e-NOS and CAT expressions were thought to be effective in the heart tissue of rats in aging process. Further detailed studies that should be designed on this subject could play important role in the diagnosis and treatment of cardiovascular diseases in which the occurrence risk is increased by the aging process.
Keywords
Klotho (KL), Endothelial Nitrite Oxide Synthase (e-NOS), Catalase (CAT), Aging, Heart Tissue
Ethical Statement
Ethics Committee Approval: All experimental procedures were performed in accordance with the standards of the European Community guidelines on the care and use of laboratory animals and approved by the Ankara University with a reference number of 2016-18-165 in accordance with the guide for the care and use of laboratory animals.
Informed Consent: Experimental study.
Peer-review: Externally peer-reviewed
Authorship Contributions
Surgical and Medical Practices: B.T., Concept: F.T., B.T., Design: F.T., B.T., Data Collection or Processing: T.Ö., Y.H., Analysis or Interpretation: F.T., T.Ö., Y.H., B.T., Literature Search: F.T., T.Ö., Y.H., Writing: F.T., T.Ö., Y.H.
Conflict of Interest: The authors have no conflicts of interest to declare.
Financial Disclosure: This study was supported by a grant from Scientific Research Projects of Ankara University (project no: 18H0230002).
References
- 1. Lopez-Otin C, Blasco MA, Partridge L, et al. The hallmarks of aging. Cell. 2013;153:1194-1217.
- 2. Kuro-o M, Matsumura Y, Aizawa H, et al. Mutation of the mouse klotho gene leads to a syndrome resembling ageing. Nature. 1997;390:45-51.
- 3. Poelzl G, Ghadge SK, Messner M, et al. Klotho is upregulated in human cardiomyopathy independently of circulating Klotho levels. Sci Rep. doi: 10.1038/s41598-018-26539-6
- 4. Ji N, Luan J, Hu F, et al. Aerobic exercise-stimulated Klotho upregulation extends life span by attenuating the excess production of reactive oxygen species in the brain and kidney. Exp Ther Med. 2018;16:3511-3517.
- 5. Wang Y, Sun Z. Current understanding of klotho. Ageing Res Rev. 2009;8:43-51.
- 6. Wang X, Hu S, Liu L. Phosphorylation and acetylation modifications of FOXO3a: Independently or synergistically? Oncol Lett. 2017;13:2867-2872.
- 7. Pathare G, Vk S, Shalia K. Emerging Roles of Klotho in Cardiovascular Diseases. JCCR. doi: 10.15406/jccr.2016.06.00228
- 8. Saito Y, Yamagishi T, Nakamura T, et al. Klotho protein protects against endothelial dysfunction. Biochem Biophys Res Commun. 1998;248:324-329.
- 9. Olgar Y, Turan B. A sodium-glucose cotransporter 2 (SGLT2) inhibitor dapagliflozin comparison with insulin shows important effects on Zn(2+)-transporters in cardiomyocytes from insulin-resistant metabolic syndrome rats through inhibition of oxidative stress (1). Can J Physiol Pharmacol. 2019;97:528-535.
- 10. Georgiou A, Lisacek-Kiosoglous A, Yiallouris A, et al. Klotho: The Protein of Faith. Ecronicon. 2017:189-223.