Makrofajlarda Neopterin ve IP-10 Üretimine İnterlökin-33’ün Etkileri

Abstract

Amaç: İnterleukin-33 (IL-33), IL-1 ailesinden pleiotropik bir sitokindir. Th2 yönünde plastisiteye yol açtığı bilinmektedir. Doğal bağışıklık sisteminin önemli hücreleri olan makrofajlar, IL-33 reseptörü ST2L’yi ifade eder ve IL-33 etkisi altında M2 makrofaj polaritesi sergiler. Makrofajlar, interferon-γ ile indüklenen bir kemokin olan interferon gama ile uyarılan protein 10 (IP-10) ve hücresel immünoreaksiyon gösteren neopterin üretir. Bu çalışmanın amacı, uyarılmamış ve farklı sitokinlerle önceden uyarılmış makrofajlarda IL-33’ün neopterin ve IP-10 üretimi üzerindeki etkilerini araştırmaktır. Gereç ve Yöntem: J774.1 makrofaj hücre dizisi kullanıldı. J774.1 makrofajları, IL-33 (100 ng/mL) ile ön işlemden sonra IFN-γ (40 U/mL) + LPS (10 ng/mL) ile uyarıldı. Nitrit seviyeleri Griess reaksiyonu ile ölçüldü. IL-33’ün polarize makrofajlar üzerindeki etkisini incelemek için J774.1 makrofajları, M1, M2a ve M2c makrofaj polarizasyonu sağlamak üzere IL-4, IL-10 ve IL-13 sitokinleri ile uyarıldı. Neopterin ve IP-10 ölçümleri ELISA yöntemi ile yapıldı. İstatistiksel analiz SPSS 18 yazılımı ile yapıldı. Bulgular: IL-33’ün, uyarılmamış ve plastisitesi etkilenmiş J774.1 hücrelerinde neopterin üretimini önemli ölçüde artırdığı bulundu. Tüm deneylerde IP-10 seviyelerinde değişiklik olmadı. Neopterin ve IP-10 seviyeleri arasında bir korelasyon bulunmadı. Sonuç: Bu çalışmamız, IL-33 sitokinin neopterin üretimi üzerindeki etkileri üzerine yapılan ilk çalışmadır. IL-33, J774.1 hücrelerinde neopterin üretiminde önemli artışa neden olmaktadır.

Keywords

• İnterlökin-33
• IP-10
• Neopterin
• Makrofaj
• ELİZA

Effects of Interleukin-33 on Neopterin and IP-10 Production in Macrophages

Abstract

Objectives: Interleukin-33 (IL-33) is a pleiotropic cytokine from the IL-1 family. It is known to cause plasticity in the Th2 direction. The macrophages, important cells of the innate immune system, express the IL-33 receptor ST2L and exhibit M2 macrophage polarity under the influence of IL-33. Macrophages produce interferon gamma-stimulated protein 10 (IP-10), an interferon-γ-induced chemokine, and neopterin which exhibits cellular immunoreaction. The aim of this study was to investigate the effects of IL-33 on neopterin and IP-10 production in unstimulated and prestimulated macrophages with different cytokines. Materials and Methods: The J774.1 macrophage cell line was used. J774.1 macrophages were stimulated by IFN-γ (40 U/mL) + LPS (10 ng/mL) after pretreatment with IL-33 (100 ng/mL). Nitrit levels were measured by the Griess reaction. To examine the effect of IL-33 on polarized macrophages, J774.1 macrophages were stimulated with IL-4, IL-10 ve IL-13 cytokines to provide M1, M2a and M2c macrophage polarization. Neopterin and IP-10 measurements were carried out by ELISA method. Statistical analysis was performed with SPSS 18 software. Results: IL-33 was found to significantly increase neopterin production in unstimulated and plasticity-affected J774.1 cells. There was no change in IP-10 levels in all experiments. No correlation was found between neopterin and IP-10 levels. Conclusion: Our present study is the first study on the effects of IL-33 cytokine on neopterin production. IL-33 causes a significant increase in neopterin production in J774.1 cells.

Keywords

• Interleukin-33
• IP-10
• Neopterin
• Macrophage
• ELISA

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