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Some biological properties of ethanol extract prepared from the aerial parts of Scutellaria albida L. subsp. condensata (Rech.f.) J.R. Edm.

Yıl 2020, Cilt: 10 Sayı: 2, 43 - 48, 28.12.2020
https://doi.org/10.17678/beuscitech.805013

Öz

Moderate effects of some plants with proven biological properties in the treatment of various diseases have increased the importance of them and the interest in alternative medicine. The members of the Lamiaceae family are one of the widely used in alternative medicine and agriculture due to their metabolite content. In order to gain valuable biological data for alternative medicine and new studies, Scutellaria albida subsp. Candensest a member of the Lamiaceae family was collected from a height of 1500 meters in Bitlis province in Turkey. Ethanol (EtOH) extract was prepared by using the aerial parts of the plant and used in all stages of the study. Firstly, the phenolic content of the extract was determined by HPLC. Myricetin and 4-Hydroxybenzoic acid at the highest concentrations were detected, but ascorbic acid, gallic acid, quercetin, and 3,4-Dihydroxybenzoic acid could not be determined in the extract. In order to test the antioxidant properties based on phenolic content, several in vitro antioxidant tests were performed and DNA protective properties were investigated. In the biological activity results, the extract was determined to have a similar antioxidant effect to standards or lower than them and exhibited relatively DNA protective activity at high concentration. Finally, the effects of the extract on some types of bacteria and fungi were investigated by the hollow agar method and 150 µL volume of the extract was shown to have better activity than ampicillin and Amikacin. Due to the limited studies on Scutellaria albida subsp Candensest, it is thought that this study will contribute to the literature.

Kaynakça

  • [1] Pagare, S., M. Bhatia, N. Tripathi, S. Pagare, and Y. Bansal. 2015. Secondary metabolites of plants and their role: Overview. Current Trends in Biotechnology and Pharmacy, 9(3), 293-304.
  • [2] Kabera, J.N., E. Semana, A.R. Mussa, and X. He. 2014. Plant secondary metabolites: biosynthesis, classification, function and pharmacological properties. J Pharm Pharmacol, 2, 377-392.
  • [3] Park, C.Y., K.-Y. Lee, K. Gul, M.S. Rahman, A.-N. Kim, J. Chun, et al. 2019. Phenolics and antioxidant activity of aqueous turmeric extracts as affected by heating temperature and time. LWT, 105, 149-155.
  • [4] Ma, Y., Y. Yang, J. Gao, J. Feng, Y. Shang, and Z. Wei. 2020. Phenolics and antioxidant activity of bamboo leaves soup as affected by in vitro digestion. Food and Chemical Toxicology, 135, 110941.
  • [5] Polat, R., U. Cakilcioglu, and F. Satil. 2013. Traditional uses of medicinal plants in Solhan (Bingol-Turkey). J Ethnopharmacol, 148(3), 951-63.
  • [6] Baser, K. and N. Kirimer. Essential oils of Lamiaceae plants of Turkey. in I International Symposium on the Labiatae: Advances in Production, Biotechnology and Utilisation 723. 2006.
  • [7] Bruno, M., F. Piozzi, and S. Rosselli. 2002. Natural and hemisynthetic neoclerodane diterpenoids from Scutellaria and their antifeedant activity. Natural Product Reports, 19(3), 357-378.
  • [8] Quave, C.L., L.R. Plano, T. Pantuso, and B.C. Bennett. 2008. Effects of extracts from Italian medicinal plants on planktonic growth, biofilm formation and adherence of methicillin-resistant Staphylococcus aureus. [Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.]. J Ethnopharmacol, 118(3), 418-28.
  • [9] Figueiredo, A.C., J.G. Barroso, L.G. Pedro, L. Salgueiro, M.G. Miguel, and M.L. Faleiro. 2008. Portuguese Thymbra and Thymus species volatiles: chemical composition and biological activities. [Review]. Curr Pharm Des, 14(29), 3120-40.
  • [10] Takaki, I., L.E. Bersani-Amado, A. Vendruscolo, S.M. Sartoretto, S.P. Diniz, C.A. Bersani-Amado, et al. 2008. Anti-inflammatory and antinociceptive effects of Rosmarinus officinalis L. essential oil in experimental animal models. [Research Support, Non-U.S. Gov't]. J Med Food, 11(4), 741-6.
  • [11] Cheung, S. and J. Tai. 2007. Anti-proliferative and antioxidant properties of rosemary Rosmarinus officinalis. [Research Support, Non-U.S. Gov't]. Oncol Rep, 17(6), 1525-31.
  • [12] Khatun, S., N.C. Chatterjee, and U. Cakilcioglu. 2011. Antioxidant activity of the medicinal plant Coleus forskohlii Briq. African Journal of Biotechnology, 10(13), 2530-2535.
  • [13] Savcı, A., E. Koçpınar, Y. Alan, and M. Kurşat. 2020. Antioxidant, antimicrobial, and DNA protection activities of some Tanacetum species and phenolic richness in their ethanolic extracts. International Food Research Journal, 27(1).
  • [14] Hindler, J. 1992. Tests to assess bactericidal activity. In Clinical Microbiology Procedures Handbook. Eisenberg HD. Washington, DC: American Society for Microbiology, pp. 5.16.14-5.16.24.
  • [15] Sagdic, O., A. Karahan, M. Ozcan, and G. Ozkan. 2003. Note: effect of some spice extracts on bacterial inhibition. Food Science and Technology International, 9(5), 353-358.
  • [16] Mitsuda, H. 1966. Antioxidative action of indole compounds during the autoxidation of linoleic acid. Eiyo to Syokuryo, 19, 210-214.
  • [17] Oyaizu, M. 1986. Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. J apanese J Nutr 44: 307-315.
  • [18] Blois, M.S. 1958. Antioxidant determinations by the use of a stable free radical. Nature, 181(4617), 1199-1200.
  • [19] Apak, R., K. Güçlü, M. Özyürek, S. Esin Karademir, and E. Erçağ. 2006. The cupric ion reducing antioxidant capacity and polyphenolic content of some herbal teas. International journal of food sciences and nutrition, 57(5-6), 292-304.
  • [20] Siddall, T.L., D.G. Ouse, Z.L. Benko, G.M. Garvin, J.L. Jackson, J.M. McQuiston, et al. 2002. Synthesis and herbicidal activity of phenyl-substituted benzoylpyrazoles. Pest Manag Sci, 58(12), 1175-86.
  • [21] Köksal, E., H. Tohma, Ö. Kılıç, Y. Alan, A. Aras, I. Gülçin, et al. 2017. Assessment of antimicrobial and antioxidant activities of Nepeta trachonitica: analysis of its phenolic compounds using HPLC-MS/MS. Scientia pharmaceutica, 85(2), 24.
  • [22] Cocan, I., E. Alexa, C. Danciu, I. Radulov, A. Galuscan, D. Obistioiu, et al. 2018. Phytochemical screening and biological activity of Lamiaceae family plant extracts. Exp Ther Med, 15(2), 1863-1870.
  • [23] Milevskaya, V., Z. Temerdashev, T. Butyl’skaya, and N. Kiseleva. 2017. Determination of phenolic compounds in medicinal plants from the Lamiaceae family. Journal of Analytical Chemistry, 72(3), 342-348.
  • [24] Skendi, A., M. Irakli, and P. Chatzopoulou. 2017. Analysis of phenolic compounds in Greek plants of Lamiaceae family by HPLC. Journal of applied research on medicinal and aromatic plants, 6, 62-69.
  • [25] Castro-Vázquez, L., M. Díaz-Maroto, M. González-Viñas, and M. Pérez-Coello. 2009. Differentiation of monofloral citrus, rosemary, eucalyptus, lavender, thyme and heather honeys based on volatile composition and sensory descriptive analysis. Food Chemistry, 112(4), 1022-1030.
  • [26] Ben Farhat, M., M.J. Jordan, R. Chaouech-Hamada, A. Landoulsi, and J.A. Sotomayor. 2009. Variations in essential oil, phenolic compounds, and antioxidant activity of tunisian cultivated Salvia officinalis L. [Research Support, Non-U.S. Gov't]. J Agric Food Chem, 57(21), 10349-56.
  • [27] Moreno, S., T. Scheyer, C.S. Romano, and A.A. Vojnov. 2006. Antioxidant and antimicrobial activities of rosemary extracts linked to their polyphenol composition. Free radical research, 40(2), 223-231.
  • [28] Yusuf, A., A. Savcı, B. ÇAKMAK, and K. Havva. 2016. Determination of The Antimicrobial and Antioxidant Activities of Satureja hortensis Ingredients. Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 21(2), 167-177.
  • [29] Duan, C., S. Matsumura, N. Kariya, M. Nishimura, and T. Shimono. 2007. In vitro antibacterial activities of Scutellaria baicalensis Georgi against cariogenic bacterial. Pediatric Dental Journal, 17(1), 58-64.
  • [30] Mahmood, S., M.Q. Hayat, A. Sadiq, S. Ishtiaq, S. Malik, and M. Ashraf. 2013. Antibacterial activity of Lallemantia royleana (Benth.) indigenous to Pakistan. African journal of microbiology research, 7(31), 4006-4009.
  • [31] Savci, A., E.F. Kocpinar, H. Budak, M. Ciftci, and M. Sisecioglu. 2020. The Effects of Amoxicillin, Cefazolin, and Gentamicin Antibiotics on the Antioxidant System in Mouse Heart Tissues. Protein Pept Lett, 27(7), 614-622.
  • [32] Kocpinar, E.F., N. Gonul Baltaci, H. Ceylan, S.N. Kalin, O. Erdogan, and H. Budak. 2020. Effect of a Prolonged Dietary Iron Intake on the Gene Expression and Activity of the Testicular Antioxidant Defense System in Rats. Biol Trace Elem Res, 195(1), 135-141.
  • [33] Zhang, H., J.M. Barceló, B. Lee, G. Kohlhagen, D.B. Zimonjic, N.C. Popescu, et al. 2001. Human mitochondrial topoisomerase I. Proceedings of the National Academy of Sciences, 98(19), 10608-10613.
  • [34] Budak, H., E.F. Kocpinar, N. Gonul, H. Ceylan, H.S. Erol, and O. Erdogan. 2014. Stimulation of gene expression and activity of antioxidant related enzyme in Sprague Dawley rat kidney induced by long-term iron toxicity. [Research Support, Non-U.S. Gov't]. Comp Biochem Physiol C Toxicol Pharmacol, 166, 44-50.
  • [35] Tepe, B., S. Degerli, S. Arslan, E. Malatyali, and C. Sarikurkcu. 2011. Determination of chemical profile, antioxidant, DNA damage protection and antiamoebic activities of Teucrium polium and Stachys iberica. Fitoterapia, 82(2), 237-246.
  • [36] Guha, G., V. Rajkumar, L. Mathew, and R.A. Kumar. 2011. The antioxidant and DNA protection potential of Indian tribal medicinal plants. Turkish Journal of Biology, 35(2), 233-242.

Some biological properties of ethanol extract prepared from the aerial parts of Scutellaria albida L. subsp. condensata (Rech.f.) J.R. Edm.

Yıl 2020, Cilt: 10 Sayı: 2, 43 - 48, 28.12.2020
https://doi.org/10.17678/beuscitech.805013

Öz

Moderate effects of some plants with proven biological properties in the treatment of various diseases have increased the importance of them and the interest in alternative medicine. The members of the Lamiaceae family are one of the widely used in alternative medicine and agriculture due to their metabolite content. In order to gain valuable biological data for alternative medicine and new studies, Scutellaria albida subsp. Candensest a member of the Lamiaceae family was collected from a height of 1500 meters in Bitlis province in Turkey. Ethanol (EtOH) extract was prepared by using the aerial parts of the plant and used in all stages of the study. Firstly, the phenolic content of the extract was determined by HPLC. Myricetin and 4-Hydroxybenzoic acid at the highest concentrations were detected, but ascorbic acid, gallic acid, quercetin, and 3,4-Dihydroxybenzoic acid could not be determined in the extract. In order to test the antioxidant properties based on phenolic content, several in vitro antioxidant tests were performed and DNA protective properties were investigated. In the biological activity results, the extract was determined to have a similar antioxidant effect to standards or lower than them and exhibited relatively DNA protective activity at high concentration. Finally, the effects of the extract on some types of bacteria and fungi were investigated by the hollow agar method and 150 µL volume of the extract was shown to have better activity than ampicillin and Amikacin. Due to the limited studies on Scutellaria albida subsp Candensest, it is thought that this study will contribute to the literature.

Kaynakça

  • [1] Pagare, S., M. Bhatia, N. Tripathi, S. Pagare, and Y. Bansal. 2015. Secondary metabolites of plants and their role: Overview. Current Trends in Biotechnology and Pharmacy, 9(3), 293-304.
  • [2] Kabera, J.N., E. Semana, A.R. Mussa, and X. He. 2014. Plant secondary metabolites: biosynthesis, classification, function and pharmacological properties. J Pharm Pharmacol, 2, 377-392.
  • [3] Park, C.Y., K.-Y. Lee, K. Gul, M.S. Rahman, A.-N. Kim, J. Chun, et al. 2019. Phenolics and antioxidant activity of aqueous turmeric extracts as affected by heating temperature and time. LWT, 105, 149-155.
  • [4] Ma, Y., Y. Yang, J. Gao, J. Feng, Y. Shang, and Z. Wei. 2020. Phenolics and antioxidant activity of bamboo leaves soup as affected by in vitro digestion. Food and Chemical Toxicology, 135, 110941.
  • [5] Polat, R., U. Cakilcioglu, and F. Satil. 2013. Traditional uses of medicinal plants in Solhan (Bingol-Turkey). J Ethnopharmacol, 148(3), 951-63.
  • [6] Baser, K. and N. Kirimer. Essential oils of Lamiaceae plants of Turkey. in I International Symposium on the Labiatae: Advances in Production, Biotechnology and Utilisation 723. 2006.
  • [7] Bruno, M., F. Piozzi, and S. Rosselli. 2002. Natural and hemisynthetic neoclerodane diterpenoids from Scutellaria and their antifeedant activity. Natural Product Reports, 19(3), 357-378.
  • [8] Quave, C.L., L.R. Plano, T. Pantuso, and B.C. Bennett. 2008. Effects of extracts from Italian medicinal plants on planktonic growth, biofilm formation and adherence of methicillin-resistant Staphylococcus aureus. [Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.]. J Ethnopharmacol, 118(3), 418-28.
  • [9] Figueiredo, A.C., J.G. Barroso, L.G. Pedro, L. Salgueiro, M.G. Miguel, and M.L. Faleiro. 2008. Portuguese Thymbra and Thymus species volatiles: chemical composition and biological activities. [Review]. Curr Pharm Des, 14(29), 3120-40.
  • [10] Takaki, I., L.E. Bersani-Amado, A. Vendruscolo, S.M. Sartoretto, S.P. Diniz, C.A. Bersani-Amado, et al. 2008. Anti-inflammatory and antinociceptive effects of Rosmarinus officinalis L. essential oil in experimental animal models. [Research Support, Non-U.S. Gov't]. J Med Food, 11(4), 741-6.
  • [11] Cheung, S. and J. Tai. 2007. Anti-proliferative and antioxidant properties of rosemary Rosmarinus officinalis. [Research Support, Non-U.S. Gov't]. Oncol Rep, 17(6), 1525-31.
  • [12] Khatun, S., N.C. Chatterjee, and U. Cakilcioglu. 2011. Antioxidant activity of the medicinal plant Coleus forskohlii Briq. African Journal of Biotechnology, 10(13), 2530-2535.
  • [13] Savcı, A., E. Koçpınar, Y. Alan, and M. Kurşat. 2020. Antioxidant, antimicrobial, and DNA protection activities of some Tanacetum species and phenolic richness in their ethanolic extracts. International Food Research Journal, 27(1).
  • [14] Hindler, J. 1992. Tests to assess bactericidal activity. In Clinical Microbiology Procedures Handbook. Eisenberg HD. Washington, DC: American Society for Microbiology, pp. 5.16.14-5.16.24.
  • [15] Sagdic, O., A. Karahan, M. Ozcan, and G. Ozkan. 2003. Note: effect of some spice extracts on bacterial inhibition. Food Science and Technology International, 9(5), 353-358.
  • [16] Mitsuda, H. 1966. Antioxidative action of indole compounds during the autoxidation of linoleic acid. Eiyo to Syokuryo, 19, 210-214.
  • [17] Oyaizu, M. 1986. Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. J apanese J Nutr 44: 307-315.
  • [18] Blois, M.S. 1958. Antioxidant determinations by the use of a stable free radical. Nature, 181(4617), 1199-1200.
  • [19] Apak, R., K. Güçlü, M. Özyürek, S. Esin Karademir, and E. Erçağ. 2006. The cupric ion reducing antioxidant capacity and polyphenolic content of some herbal teas. International journal of food sciences and nutrition, 57(5-6), 292-304.
  • [20] Siddall, T.L., D.G. Ouse, Z.L. Benko, G.M. Garvin, J.L. Jackson, J.M. McQuiston, et al. 2002. Synthesis and herbicidal activity of phenyl-substituted benzoylpyrazoles. Pest Manag Sci, 58(12), 1175-86.
  • [21] Köksal, E., H. Tohma, Ö. Kılıç, Y. Alan, A. Aras, I. Gülçin, et al. 2017. Assessment of antimicrobial and antioxidant activities of Nepeta trachonitica: analysis of its phenolic compounds using HPLC-MS/MS. Scientia pharmaceutica, 85(2), 24.
  • [22] Cocan, I., E. Alexa, C. Danciu, I. Radulov, A. Galuscan, D. Obistioiu, et al. 2018. Phytochemical screening and biological activity of Lamiaceae family plant extracts. Exp Ther Med, 15(2), 1863-1870.
  • [23] Milevskaya, V., Z. Temerdashev, T. Butyl’skaya, and N. Kiseleva. 2017. Determination of phenolic compounds in medicinal plants from the Lamiaceae family. Journal of Analytical Chemistry, 72(3), 342-348.
  • [24] Skendi, A., M. Irakli, and P. Chatzopoulou. 2017. Analysis of phenolic compounds in Greek plants of Lamiaceae family by HPLC. Journal of applied research on medicinal and aromatic plants, 6, 62-69.
  • [25] Castro-Vázquez, L., M. Díaz-Maroto, M. González-Viñas, and M. Pérez-Coello. 2009. Differentiation of monofloral citrus, rosemary, eucalyptus, lavender, thyme and heather honeys based on volatile composition and sensory descriptive analysis. Food Chemistry, 112(4), 1022-1030.
  • [26] Ben Farhat, M., M.J. Jordan, R. Chaouech-Hamada, A. Landoulsi, and J.A. Sotomayor. 2009. Variations in essential oil, phenolic compounds, and antioxidant activity of tunisian cultivated Salvia officinalis L. [Research Support, Non-U.S. Gov't]. J Agric Food Chem, 57(21), 10349-56.
  • [27] Moreno, S., T. Scheyer, C.S. Romano, and A.A. Vojnov. 2006. Antioxidant and antimicrobial activities of rosemary extracts linked to their polyphenol composition. Free radical research, 40(2), 223-231.
  • [28] Yusuf, A., A. Savcı, B. ÇAKMAK, and K. Havva. 2016. Determination of The Antimicrobial and Antioxidant Activities of Satureja hortensis Ingredients. Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 21(2), 167-177.
  • [29] Duan, C., S. Matsumura, N. Kariya, M. Nishimura, and T. Shimono. 2007. In vitro antibacterial activities of Scutellaria baicalensis Georgi against cariogenic bacterial. Pediatric Dental Journal, 17(1), 58-64.
  • [30] Mahmood, S., M.Q. Hayat, A. Sadiq, S. Ishtiaq, S. Malik, and M. Ashraf. 2013. Antibacterial activity of Lallemantia royleana (Benth.) indigenous to Pakistan. African journal of microbiology research, 7(31), 4006-4009.
  • [31] Savci, A., E.F. Kocpinar, H. Budak, M. Ciftci, and M. Sisecioglu. 2020. The Effects of Amoxicillin, Cefazolin, and Gentamicin Antibiotics on the Antioxidant System in Mouse Heart Tissues. Protein Pept Lett, 27(7), 614-622.
  • [32] Kocpinar, E.F., N. Gonul Baltaci, H. Ceylan, S.N. Kalin, O. Erdogan, and H. Budak. 2020. Effect of a Prolonged Dietary Iron Intake on the Gene Expression and Activity of the Testicular Antioxidant Defense System in Rats. Biol Trace Elem Res, 195(1), 135-141.
  • [33] Zhang, H., J.M. Barceló, B. Lee, G. Kohlhagen, D.B. Zimonjic, N.C. Popescu, et al. 2001. Human mitochondrial topoisomerase I. Proceedings of the National Academy of Sciences, 98(19), 10608-10613.
  • [34] Budak, H., E.F. Kocpinar, N. Gonul, H. Ceylan, H.S. Erol, and O. Erdogan. 2014. Stimulation of gene expression and activity of antioxidant related enzyme in Sprague Dawley rat kidney induced by long-term iron toxicity. [Research Support, Non-U.S. Gov't]. Comp Biochem Physiol C Toxicol Pharmacol, 166, 44-50.
  • [35] Tepe, B., S. Degerli, S. Arslan, E. Malatyali, and C. Sarikurkcu. 2011. Determination of chemical profile, antioxidant, DNA damage protection and antiamoebic activities of Teucrium polium and Stachys iberica. Fitoterapia, 82(2), 237-246.
  • [36] Guha, G., V. Rajkumar, L. Mathew, and R.A. Kumar. 2011. The antioxidant and DNA protection potential of Indian tribal medicinal plants. Turkish Journal of Biology, 35(2), 233-242.
Toplam 36 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Makaleler
Yazarlar

Enver Fehim Koçpınar 0000-0002-6031-4664

Murat Kürşat 0000-0002-0861-4213

Ahmet Savcı 0000-0002-9609-785X

Yusuf Alan 0000-0003-0007-0212

Yayımlanma Tarihi 28 Aralık 2020
Gönderilme Tarihi 4 Ekim 2020
Yayımlandığı Sayı Yıl 2020 Cilt: 10 Sayı: 2

Kaynak Göster

IEEE E. F. Koçpınar, M. Kürşat, A. Savcı, ve Y. Alan, “ Edm”., Bitlis Eren University Journal of Science and Technology, c. 10, sy. 2, ss. 43–48, 2020, doi: 10.17678/beuscitech.805013.