Synthesis and Characterization of Curcumin-Lysine Carbon Dots
Year 2025,
Volume: 6 Issue: 2, 61 - 66, 29.12.2025
İrem Yakar
,
Selin Şen
,
Ayben Top
Abstract
Antibacterial materials are designed to inhibit or kill bacterial growth, making them essential in applications where hygiene and infection control are critical, such as wound care and medical devices. Carbon dots (CDs) are nanoscale materials with unique optical properties, chemical stability, biocompatibility, and ease of synthesis and functionalization. The properties of CDs are primarily determined by the precursors used for synthesis. In this study, we synthesized CDs from curcumin and L-lysine using a furnace-based method. Structural and optical characterization confirmed successful carbonization. The synthesized CDs exhibited mild antibacterial activity with an average inhibition zone of approximately 0.5 mm compared to the blank disc against the common wound pathogen, Staphylococcus aureus. They also supported viability above 80% up to 1mg/ml concentration. These findings indicate the potential of these carbon dots as biocompatible antibacterial agents for wound healing.
References
-
Cui L, Ren X, Sun M, Liu H, Xia L (2021) Carbon Dots: Synthesis, Properties and Applications. Nanomaterials 2021, Vol 11, Page 3419 11:3419.
-
Darvishi E, Shekarbeygi Z, Yousefinezhad S, Izadi Z, Saboury AA, Derakhshankhah H, Varnamkhasti BS (2021) Green synthesis of nanocarbon dots using hydrothermal carbonization of lysine amino acid and its application in detection of duloxetine. Journal of the Iranian Chemical Society 18:2863–2872.
-
Fibriani A, Taharuddin AAP, Stephanie R, Yamahoki N, Laurelia J, Wisnuwardhani PH, Agustiyanti DF, Angelina M, Rubiyana Y, Ningrum RA, Wardiana A, Iskandar F, Permatasari FA, Giri-Rachman EA (2023) Curcumin-derived carbon-dots as a potential COVID-19 antiviral drug. Heliyon 9:e20089. https://doi.org/10.1016/J.HELIYON.2023.E20089
-
Guo B, Liu G, Hu C, Lei B, Liu Y (2022) The structural characteristics and mechanisms of antimicrobial carbon dots: a mini review. Mater Adv 3:7726–7741.
-
Jian HJ, Anand A, Lai JY, Unnikrishnan B, Chang HT, Harroun SG, Huang CC (2023) In Situ Hybridization of Polymeric Curcumin to Arginine-Derived Carbon Quantum Dots for Synergistic Treatment of Bacterial Infections. ACS Appl Mater Interfaces 15:26457–26471.
-
Jiang Y, Xu X, Lu J, Yin C, Li G, Bai L, Zhang T, Mo J, Wang X, Shi Q, Wang T, Zhou Q (2023) Development of ε-poly(L-lysine) carbon dots-modified magnetic nanoparticles and their applications as novel antibacterial agents. Front Chem 11:1184592.
-
Le NMN, Steinbring C, Le-Vinh B, Jalil A, Matuszczak B, Bernkop-Schnürch A (2021) Polyphosphate coatings: A promising strategy to overcome the polycation dilemma. J Colloid Interface Sci 587:279–289.
-
Li P, Han F, Cao W, Zhang G, Li J, Zhou J, Gong X, Turnbull G, Shu W, Xia L, Fang B, Xing X, Li B (2020) Carbon quantum dots derived from lysine and arginine simultaneously scavenge bacteria and promote tissue repair. Appl Mater Today 19:100601.
-
Liang J, Li W, Chen J, Huang X, Liu Y, Zhang X, Shu W, Lei B, Zhang H (2021) Antibacterial Activity and Synergetic Mechanism of Carbon Dots against Gram-Positive and -Negative Bacteria. ACS Appl Bio Mater 4:6937–6945.
-
Lu F, Ma Y, Wang H, Zhang M, Wang B, Zhang Y, Huang H, Liao F, Liu Y, Kang Z (2021) Water-solvable carbon dots derived from curcumin and citric acid with enhanced broad-spectrum antibacterial and antibiofilm activity. Mater Today Commun 26:102000.
-
Margaryan I V., Mitroshin AM, Dubavik AY, Kundelev E V. (2024) Investigation of the Optical Properties of Aminated Carbon Dots Based on Citric Acid and Ethylenediamine. Opt Spectrosc 132:273–278.
-
Miao H, Wang P, Wu J, Li X, Du Y, Yan H, You Q, Dong W, Li L (2025) Highly efficient and broad-spectrum antibacterial carbon dots combat antibiotic resistance. Talanta 281:126926.
-
Ozyurt D, Kobaisi M Al, Hocking RK, Fox B (2023) Properties, synthesis, and applications of carbon dots: A review. Carbon Trends 12:100276.
-
Pal T, Mohiyuddin S, Packirisamy G (2018) Facile and Green Synthesis of Multicolor Fluorescence Carbon Dots from Curcumin: In Vitro and in Vivo Bioimaging and Other Applications. ACS Omega 3:831–843.
-
Sırma Tarım B, Tamburacı S, Uysal B, Top A (2025) Integration of Leu-Asp-Val Cell Attachment Motif into Self-Assembling Peptide Sequences for Nanofibrillar Hydrogel Formation in Wound Healing. ACS Appl Nano Mater 8:5302–5314.
-
Sperandio F, Huang Y-Y, Hamblin M (2013) Antimicrobial photodynamic therapy to kill Gram-negative bacteria. Recent Pat Antiinfect Drug Discov 8:108–120.
-
Thulasi S, Kathiravan A, Asha Jhonsi M (2020) Fluorescent Carbon Dots Derived from Vehicle Exhaust Soot and Sensing of Tartrazine in Soft Drinks. ACS Omega 5:7025–7031.
-
Vibhute A, Nille O, Kolekar G, Rohiwal S, Patil S, Lee S, Tiwari AP (2022) Fluorescent Carbon Quantum Dots Functionalized by Poly L-Lysine: Efficient Material for Antibacterial, Bioimaging and Antiangiogenesis Applications. J Fluoresc 32:1789–1800.
-
Wang Y, Hu A (2014) Carbon quantum dots: synthesis, properties and applications. J Mater Chem C Mater 2:6921–6939.
-
Yadav H, Rout D, Upadhyaya AK, Agarwala P, Sharma A, Sasmal DK (2023) Carbon quantum dots for efficient delivery of curcumin in live cell. Chemical Physics Impact 7:100279.
-
Zhang X, Wu P, Hao X, Liu J, Huang Z, Weng S, Chen W, Huang L, Huang J (2023) Quaternized carbon dots with enhanced antimicrobial ability towards Gram-negative bacteria for the treatment of acute peritonitis caused by E. coli. J Mater Chem B 11:7696–7706.
-
Zhang Y, Wang J, Wu X, Chang R, Luo H, Yang J, Wu Q, Xu Z, Zhong Y (2025) Synthesis of carbon dots as an antibacterial and antioxidant agent. Front Chem 13:1627543.