Evaluation of the Photo-inactivation Efficacy and Killing Kinetics of 405 nm Visible Light against Multidrug-Resistant Acinetobacter baumannii

Yeliz Durgun; Mahmut Durgun

doi:10.51539/biotech.1939065

Evaluation of the Photo-inactivation Efficacy and Killing Kinetics of 405 nm Visible Light against Multidrug-Resistant Acinetobacter baumannii

Abstract

The Multidrug-resistant (MDR) Acinetobacter baumannii poses a critical threat in clinical settings due to its high environmental stability and resistance to conventional antibiotics. Antimicrobial blue light, particularly at 405 nm, has emerged as a promising non-invasive decontamination strategy. Objective: This study aimed to evaluate the photo-inactivation efficacy and determine the killing kinetics of 405 nm light against A. baumannii. Methods: Bacterial samples were exposed to a 405 nm light source for up to 120 minutes. Survival rates were compared with a dark control group, and the data were modeled using first-order Chick-Watson kinetics. Results: A significant time-dependent reduction in bacterial viability was observed. After 120 minutes of exposure, 405 nm light achieved a 98.6% inactivation success compared to the control group (p < 0.01). Kinetic analysis revealed an inactivation rate constant (k) of 0.0291 min-1 with a high coefficient of determination (R2 = 0.8888). The calculated half-life (t1/2) and decimal reduction time (D-value) were 23.84 and 79.19 minutes, respectively.These results demonstrate that 405 nm irradiation provides a potent and mathematically predictable bactericidal effect against MDR A. baumannii. The findings suggest that 405 nm light therapy serves as a viable alternative for the environmental decontamination of highly resistant pathogens.

Keywords

References

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Details

Primary Language

English

Subjects

Bioinformatic Methods Development, Microtechnologies

Journal Section

Research Article

Authors

Yeliz Durgun ^*
0000-0003-3834-5533
Türkiye

Mahmut Durgun
0000-0002-5010-687X
Türkiye

Publication Date

June 29, 2026

Submission Date

April 28, 2026

Acceptance Date

June 13, 2026

Published in Issue

Year 2026 Volume: 7 Number: 1

DOI

https://doi.org/10.51539/biotech.1939065

IZ

https://izlik.org/JA23GK78NJ

Cite

RIS / Bibtex

APA

Durgun, Y., & Durgun, M. (2026). Evaluation of the Photo-inactivation Efficacy and Killing Kinetics of 405 nm Visible Light against Multidrug-Resistant Acinetobacter baumannii. Bulletin of Biotechnology, 7(1), 15-20. https://doi.org/10.51539/biotech.1939065

AMA

1.Durgun Y, Durgun M. Evaluation of the Photo-inactivation Efficacy and Killing Kinetics of 405 nm Visible Light against Multidrug-Resistant Acinetobacter baumannii. Bull. Biotechnol. 2026;7(1):15-20. doi:10.51539/biotech.1939065

Chicago

Durgun, Yeliz, and Mahmut Durgun. 2026. “Evaluation of the Photo-Inactivation Efficacy and Killing Kinetics of 405 Nm Visible Light Against Multidrug-Resistant Acinetobacter Baumannii”. Bulletin of Biotechnology 7 (1): 15-20. https://doi.org/10.51539/biotech.1939065.

EndNote

Durgun Y, Durgun M (June 1, 2026) Evaluation of the Photo-inactivation Efficacy and Killing Kinetics of 405 nm Visible Light against Multidrug-Resistant Acinetobacter baumannii. Bulletin of Biotechnology 7 1 15–20.

IEEE

[1]Y. Durgun and M. Durgun, “Evaluation of the Photo-inactivation Efficacy and Killing Kinetics of 405 nm Visible Light against Multidrug-Resistant Acinetobacter baumannii”, Bull. Biotechnol., vol. 7, no. 1, pp. 15–20, June 2026, doi: 10.51539/biotech.1939065.

ISNAD

Durgun, Yeliz - Durgun, Mahmut. “Evaluation of the Photo-Inactivation Efficacy and Killing Kinetics of 405 Nm Visible Light Against Multidrug-Resistant Acinetobacter Baumannii”. Bulletin of Biotechnology 7/1 (June 1, 2026): 15-20. https://doi.org/10.51539/biotech.1939065.

JAMA

1.Durgun Y, Durgun M. Evaluation of the Photo-inactivation Efficacy and Killing Kinetics of 405 nm Visible Light against Multidrug-Resistant Acinetobacter baumannii. Bull. Biotechnol. 2026;7:15–20.

MLA

Durgun, Yeliz, and Mahmut Durgun. “Evaluation of the Photo-Inactivation Efficacy and Killing Kinetics of 405 Nm Visible Light Against Multidrug-Resistant Acinetobacter Baumannii”. Bulletin of Biotechnology, vol. 7, no. 1, June 2026, pp. 15-20, doi:10.51539/biotech.1939065.

Vancouver

1.Yeliz Durgun, Mahmut Durgun. Evaluation of the Photo-inactivation Efficacy and Killing Kinetics of 405 nm Visible Light against Multidrug-Resistant Acinetobacter baumannii. Bull. Biotechnol. 2026 Jun. 1;7(1):15-20. doi:10.51539/biotech.1939065