Yıl 2019, Cilt 59 , Sayı 3, Sayfalar 31 - 37 2019-09-30

Fusarium species isolated from wheat samples showing root and crown rot
Güneydoğu Anadolu Bölgesi’nde kök ve kök boğazı çürüklüğü belirtileri gösteren buğday örneklerinden izole edilen Fusarium türleri

Fatih ÖLMEZ [1] , Berna TUNALI [2]


Wheat is one of the most largely grown cereals in Turkey and it is very important for meet calorie requirements of humans and animals. However, due to numerous fungal diseases suffered, the wheat supply is threatened. Fusarium spp., known as the big portion of plant parasitic fungi, causes some quality and quantity problems on wheat production. This large genus has over one hundred sub-species, therefore, it is so difficult to diagnose in species level. Using certain molecular and microscopic approaches, this study aimed at determining the Fusarium spp., causing root and crown rot on wheat in Southeast Anatolia. Molecularly, using polymerase chain reaction (PCR) experiment with some species-specific primers and sequencing of some part of ribosomal RNA region after amplification by PCR were used to differentiate the species. Additionally, colony and different spore characteristics of cultures were used to diagnose some Fusarium species microscopically. At the end of the study, 143 Fusarium isolates including 19 species were obtained from wheat producing areas in Diyarbakır, Şanlıurfa, Mardin, and Adıyaman provinces. Although the most frequently isolated species was F. proliferatum at the rate of 17.4%, F. pseudograminearum and F. culmorum, which were the most important crown rot pathogens, were isolated at 13% of the total isolated Fusarium isolates.


Türkiye’de insan ve hayvan beslenmesinde kalori ihtiyacının çok önemli bir bölümünü karşılayan buğday ülkemizde en geniş alanda yetiştirilen tahıldır. Buğday arzını tehdit eden birçok fungal hastalık olmasına rağmen, Fusarium türleri buğdayda kalite ve kantite problemlerine neden olan önemli bir patojenik gruptur. Fusarium cinsine ait 100’den fazla tür vardır ve bu yüzden tür düzeyinde ayrımları kolay değildir. Bu çalışma Güneydoğu Anadolu Bölgesi’nde buğdayda kök ve kök boğazı çürüklüğüne neden olan Fusarium türlerinin belirlenmesini amaçlamıştır. Tür düzeyinde moleküler teşhisler için bazı tür-spesifik primerler ve bazı ribozomal RNA bölgelerinin dizilenmesi ile gerçekleştirilmiştir. Bunlara ek olarak bazı türlerin teşhisleri, koloni ve spor karakterleri kullanılarak, mikroskobik olarak yapılmıştır. Çalışma sonunda Diyarbakır, Şanlıurfa, Mardin ve Adıyaman illeri buğday üretim alanlarında 19 farklı türe ait 143 Fusarium türü belirlenmiştir. En yaygın tür %17.4 ile F. proliferatum olmasına rağmen, en önemli buğday kök boğazı çürüklüğü patojenlerinden olan F. pseudograminearum ve F. culmorum izole edilen Fusarium türlerinin %13’ünü oluşturmuştur.

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Birincil Dil en
Konular Fen
Bölüm Makaleler
Yazarlar

Orcid: 0000-0001-7016-2708
Yazar: Fatih ÖLMEZ (Sorumlu Yazar)
Kurum: ŞIRNAK UNIVERSITY, FACULTY OF AGRICULTURE, DEPARTMENT OF PLANT PATHOLOGY
Ülke: Turkey


Yazar: Berna TUNALI
Kurum: ONDOKUZ MAYIS UNIVERSITY, FACULTY OF AGRICULTURE, DEPARTMENT OF PLANT PATHOLOGY
Ülke: Turkey


Tarihler

Yayımlanma Tarihi : 30 Eylül 2019

Bibtex @araştırma makalesi { bitkorb515066, journal = {Bitki Koruma Bülteni}, issn = {0406-3597}, eissn = {1308-8122}, address = {}, publisher = {Zirai Mücadele Merkez Araştırma Enstitüsü}, year = {2019}, volume = {59}, pages = {31 - 37}, doi = {10.16955/bitkorb.515066}, title = {Fusarium species isolated from wheat samples showing root and crown rot}, key = {cite}, author = {ÖLMEZ, Fatih and TUNALI, Berna} }
APA ÖLMEZ, F , TUNALI, B . (2019). Fusarium species isolated from wheat samples showing root and crown rot. Bitki Koruma Bülteni , 59 (3) , 31-37 . DOI: 10.16955/bitkorb.515066
MLA ÖLMEZ, F , TUNALI, B . "Fusarium species isolated from wheat samples showing root and crown rot". Bitki Koruma Bülteni 59 (2019 ): 31-37 <https://dergipark.org.tr/tr/pub/bitkorb/issue/48507/515066>
Chicago ÖLMEZ, F , TUNALI, B . "Fusarium species isolated from wheat samples showing root and crown rot". Bitki Koruma Bülteni 59 (2019 ): 31-37
RIS TY - JOUR T1 - Fusarium species isolated from wheat samples showing root and crown rot AU - Fatih ÖLMEZ , Berna TUNALI Y1 - 2019 PY - 2019 N1 - doi: 10.16955/bitkorb.515066 DO - 10.16955/bitkorb.515066 T2 - Bitki Koruma Bülteni JF - Journal JO - JOR SP - 31 EP - 37 VL - 59 IS - 3 SN - 0406-3597-1308-8122 M3 - doi: 10.16955/bitkorb.515066 UR - https://doi.org/10.16955/bitkorb.515066 Y2 - 2019 ER -
EndNote %0 Bitki Koruma Bülteni Fusarium species isolated from wheat samples showing root and crown rot %A Fatih ÖLMEZ , Berna TUNALI %T Fusarium species isolated from wheat samples showing root and crown rot %D 2019 %J Bitki Koruma Bülteni %P 0406-3597-1308-8122 %V 59 %N 3 %R doi: 10.16955/bitkorb.515066 %U 10.16955/bitkorb.515066
ISNAD ÖLMEZ, Fatih , TUNALI, Berna . "Fusarium species isolated from wheat samples showing root and crown rot". Bitki Koruma Bülteni 59 / 3 (Eylül 2019): 31-37 . https://doi.org/10.16955/bitkorb.515066
AMA ÖLMEZ F , TUNALI B . Fusarium species isolated from wheat samples showing root and crown rot. Bitki Koruma Bülteni. 2019; 59(3): 31-37.
Vancouver ÖLMEZ F , TUNALI B . Fusarium species isolated from wheat samples showing root and crown rot. Bitki Koruma Bülteni. 2019; 59(3): 37-31.