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Characterization of the Elemental Constituents, Phenolic Profile, and Antioxidant Capacity of Cultivated Horehound (Marrubium vulgare L.) Leaves from Van, Türkiye

Yıl 2025, Cilt: 8 Sayı: 4, 505 - 509, 15.07.2025
https://doi.org/10.47115/bsagriculture.1701538

Öz

Horehound (Marrubium vulgare L.) is a perennial plant species that originated in the Mediterranean-Central Asian region and has since become globally distributed. Marrubium vulgare L. is a plant species that contains a diverse array of bioactive compounds, including alkaloids, sterols, steroids, terpenoids, saponins, flavonoids, tannins, and various phenolic compounds. The flowering aerial parts or leaves of this plant are widely used in traditional medicine throughout Anatolia and other regions for their aromatic, antipyretic, diuretic, and carminative properties, as well as their effectiveness in treating conditions such as colds, coughs, and respiratory disorders. This study aim to explore the total phenolic and flavonoid content, total antioxidant capacity, macro-micro element and heavy metal composition of cultivated M. vulgare L. The results of the study revealed that the total flavonoid content was 8.64 mg QE 100 g-1, the total phenolic content was 133.18 mg GAE g-1, and the total antioxidant activity was 53.45 μmol TE g-1. The study also characterized the total ash and dry matter content and mineral composition of the species, including both macronutrients and micronutrients (Ca, K, Mg, Na, Fe, Zn, Cu, Mn, Mo) as well as the presence of heavy metals (Cd, Co, Se, Ni, Pb, As).

Etik Beyan

Since no studies involving humans or animals were conducted, ethical committee approval was not required for this study.

Kaynakça

  • Aćimović M, Jeremić K, Salaj N, Gavarić N, Kiprovski B, Sikora V, Zeremski T. 2020. Marrubium vulgare L.: A phytochemical and pharmacological overview. Molecules, 25(12): 2898.
  • Ahmed AB, Kebi T. 2023. Phytochemical screening and evaluation of heavy metals in a medicinal plant Marrubium vulgare L. Int J Circuits Syst Signal Process, 17: 107-111.
  • Al-Snafi AE, Al-Saedy HA, Talab TA, Majid WJ, Batiha GES, Abofazi JS. 2021. The bioactive ingredients and therapeutic effects of Marrubium vulgare - A review. Int J Biol Pharm Sci Arch, 1(2): 9-21.
  • Amessis-Ouchemoukh N, Abu-Reidah IM, Quirantes-Piné R, Madani K, Segura-Carretero A. 2014. Phytochemical profiling, in vitro evaluation of total phenolic contents and antioxidant properties of Marrubium vulgare (horehound) leaves of plants growing in Algeria. Ind Crops Prod, 61: 120-129.
  • Başgel S, Erdemoğlu SB. 2006. Determination of mineral and trace elements in some medicinal herbs and their infusions consumed in Turkey. Sci Total Environ, 359(1-3): 82-89.
  • Bautista I, Boscaiu M, Lidón A, Llinares JV, Lull C, Donat MP, Vicente O. 2016. Environmentally induced changes in antioxidant phenolic compounds levels in wild plants. Acta Physiol Plant, 38: 1-15.
  • Boudjelal A, Henchiri C, Siracusa L, Sari M, Ruberto G. 2012. Compositional analysis and in vivo anti-diabetic activity of wild Algerian Marrubium vulgare L. infusion. Fitoterapia, 83: 286-292.
  • Boulila A, Sanaa A, Ben Salem I, Rokbeni N, Rabet YM, Hosni K, Fernandez X. 2015. Antioxidant properties and phenolic variation in wild populations of Marrubium vulgare L. (Lamiaceae). Ind Crops Prod, 76: 616-622.
  • Chedia A, Ghazghazi H, Brahim H, Abderrazak M. 2014. Total phenolic content, antioxidant and antibacterial activities of Marrubium vulgare methanolic extract. Tunisian J Med Plants Nat Prod, 11: 1-8.
  • Das S, Roy A, Das PK. 2014. Ethnomedicinal uses of plants for wound healing in Khargone district, MP: A survey over Nimari communities. Res J Pharmacol Pharmacodyn, 6: 21-29.
  • Demiroz Akbulut T, Aydin Kose F, Demirci B, Baykan S. 2023. Chemical profile and cytotoxicity evaluation of aerial parts of Marrubium vulgare L. from different locations in Turkey. Chem Biodivers, 20(4): e202201188.
  • Guedri Mkaddem M, Zrig A, Ben Abdallah M, Romdhane M, Okla MK, Al-Hashimi A, AbdElgawad H. 2022. Variation of the chemical composition of essential oils and total phenols content in natural populations of Marrubium vulgare L. Plants, 11(5): 612.
  • Kacar B, İnal A. 2008. Plant analysis. Nobel Press, Ankara, Türkiye, pp: 138.
  • Knoss W. 1999. Marrubium vulgare (white horehound): In vitro culture, and the production of diterpene marrubiin and other secondary metabolites. In: Bajaj YPS, ed. Medicinal and Aromatic Plants XI. Springer, Berlin/Heidelberg, Almanya, pp: 274-289.
  • Lodhi S, Vadnere GP, Sharma VK, Usman MR. 2017. Marrubium vulgare L.: A review on phytochemical and pharmacological aspects. J Intercult Ethnopharmacol, 6(4): 429-452.
  • Lutz M, Jorquera K, Cancino B, Ruby R, Henriquez C. 2011. Phenolics and antioxidant capacity of table grape (Vitis vinifera L.) cultivars grown in Chile. J Food Sci, 76: 1088-1093.
  • Maiti R, González Rodríguez H, Kumari A, Chandra Sarkar N. 2016. Macro and micro-nutrient contents of 18 medicinal plants used traditionally to alleviate diabetes in Nuevo Leon, northeast of Mexico. Pak J Bot, 48(1): 271-276.
  • Matkowski A, Piotrowska M. 2006. Antioxidant and free radical scavenging activities of some medicinal plants from the Lamiaceae. Fitoterapia, 77(5): 346-353.
  • Matkowski A, Tasarz P, Szypuła E. 2008. Antioxidant activity of herb extracts from five medicinal plants from Lamiaceae, subfamily Lamioideae. J Med Plant Res, 2(11): 321-330.
  • Michalak M, Stryjecka M, Zagórska-Dziok M, Żarnowiec P. 2024. Biological activity of horehound (Marrubium vulgare L.) herb grown in Poland and its phytochemical composition. Pharmaceuticals, 17(6): 780.
  • Michalak M. 2022. Plant-derived antioxidants: Significance in skin health and the ageing process. Int J Mol Sci, 23: 585.
  • Nwozo OS, Effiong EM, Aja PM, Awuchi CG. 2023. Antioxidant, phytochemical, and therapeutic properties of medicinal plants: a review. Int J Food Prop, 26(1): 359-388.
  • Obanda M, Owuor PO, Taylor SJ. 1997. Flavanol composition and caffeine content of green leaf as quality potential indicators of Kenyan black teas. J Sci Food Agric, 74(2): 209-215.
  • Quettier-Deleu C, Gressier B, Vasseur J, Dine T, Brunet C, Luyckx M, Trotin F. 2000. Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. J Ethnopharmacol, 72(1–2): 35-42.
  • Rezgui M, Basma M, Neng N, Nogueira JM, Bettaieb Ben-Kaab L, Machado Araújo ME. 2021. Evaluation of Marrubium vulgare growing wild in Tunisia for its potential as a dietary supplement. Foods, 10(11): 2864.
  • Sekeroglu N, Ozkutlu F, Kara SM, Ozguven M. 2008. Determination of cadmium and selected micronutrients in commonly used and traded medicinal plants in Turkey. J Sci Food Agric, 88(1): 86-90.
  • Shallari S, Schwartz C, Hasko A, Morel JL. 1998. Heavy metals in soils and plants of serpentine and industrial sites of Albania. Sci Total Environ, 209(2–3): 133-142.
  • Tercan H, Ayanoglu F, Bahadirli NP. 2016. Determination of heavy metal contents and some basic aspects of widely used herbal teas in Turkey. Nature, 3(4): 1019-1022.
  • VanderJagt TJ, Ghattas R, VanderJagt DJ, Crossey M, Glew RH. 2002. Comparison of the total antioxidant content of 30 widely used medicinal plants of New Mexico. Life Sci, 70(9): 1035-1040.
  • Wojdyło A, Oszmiański J, Czemerys R. 2007. Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chem, 105: 940-949.

Characterization of the Elemental Constituents, Phenolic Profile, and Antioxidant Capacity of Cultivated Horehound (Marrubium vulgare L.) Leaves from Van, Türkiye

Yıl 2025, Cilt: 8 Sayı: 4, 505 - 509, 15.07.2025
https://doi.org/10.47115/bsagriculture.1701538

Öz

Horehound (Marrubium vulgare L.) is a perennial plant species that originated in the Mediterranean-Central Asian region and has since become globally distributed. Marrubium vulgare L. is a plant species that contains a diverse array of bioactive compounds, including alkaloids, sterols, steroids, terpenoids, saponins, flavonoids, tannins, and various phenolic compounds. The flowering aerial parts or leaves of this plant are widely used in traditional medicine throughout Anatolia and other regions for their aromatic, antipyretic, diuretic, and carminative properties, as well as their effectiveness in treating conditions such as colds, coughs, and respiratory disorders. This study aim to explore the total phenolic and flavonoid content, total antioxidant capacity, macro-micro element and heavy metal composition of cultivated M. vulgare L. The results of the study revealed that the total flavonoid content was 8.64 mg QE 100 g-1, the total phenolic content was 133.18 mg GAE g-1, and the total antioxidant activity was 53.45 μmol TE g-1. The study also characterized the total ash and dry matter content and mineral composition of the species, including both macronutrients and micronutrients (Ca, K, Mg, Na, Fe, Zn, Cu, Mn, Mo) as well as the presence of heavy metals (Cd, Co, Se, Ni, Pb, As).

Etik Beyan

Since no studies involving humans or animals were conducted, ethical committee approval was not required for this study.

Kaynakça

  • Aćimović M, Jeremić K, Salaj N, Gavarić N, Kiprovski B, Sikora V, Zeremski T. 2020. Marrubium vulgare L.: A phytochemical and pharmacological overview. Molecules, 25(12): 2898.
  • Ahmed AB, Kebi T. 2023. Phytochemical screening and evaluation of heavy metals in a medicinal plant Marrubium vulgare L. Int J Circuits Syst Signal Process, 17: 107-111.
  • Al-Snafi AE, Al-Saedy HA, Talab TA, Majid WJ, Batiha GES, Abofazi JS. 2021. The bioactive ingredients and therapeutic effects of Marrubium vulgare - A review. Int J Biol Pharm Sci Arch, 1(2): 9-21.
  • Amessis-Ouchemoukh N, Abu-Reidah IM, Quirantes-Piné R, Madani K, Segura-Carretero A. 2014. Phytochemical profiling, in vitro evaluation of total phenolic contents and antioxidant properties of Marrubium vulgare (horehound) leaves of plants growing in Algeria. Ind Crops Prod, 61: 120-129.
  • Başgel S, Erdemoğlu SB. 2006. Determination of mineral and trace elements in some medicinal herbs and their infusions consumed in Turkey. Sci Total Environ, 359(1-3): 82-89.
  • Bautista I, Boscaiu M, Lidón A, Llinares JV, Lull C, Donat MP, Vicente O. 2016. Environmentally induced changes in antioxidant phenolic compounds levels in wild plants. Acta Physiol Plant, 38: 1-15.
  • Boudjelal A, Henchiri C, Siracusa L, Sari M, Ruberto G. 2012. Compositional analysis and in vivo anti-diabetic activity of wild Algerian Marrubium vulgare L. infusion. Fitoterapia, 83: 286-292.
  • Boulila A, Sanaa A, Ben Salem I, Rokbeni N, Rabet YM, Hosni K, Fernandez X. 2015. Antioxidant properties and phenolic variation in wild populations of Marrubium vulgare L. (Lamiaceae). Ind Crops Prod, 76: 616-622.
  • Chedia A, Ghazghazi H, Brahim H, Abderrazak M. 2014. Total phenolic content, antioxidant and antibacterial activities of Marrubium vulgare methanolic extract. Tunisian J Med Plants Nat Prod, 11: 1-8.
  • Das S, Roy A, Das PK. 2014. Ethnomedicinal uses of plants for wound healing in Khargone district, MP: A survey over Nimari communities. Res J Pharmacol Pharmacodyn, 6: 21-29.
  • Demiroz Akbulut T, Aydin Kose F, Demirci B, Baykan S. 2023. Chemical profile and cytotoxicity evaluation of aerial parts of Marrubium vulgare L. from different locations in Turkey. Chem Biodivers, 20(4): e202201188.
  • Guedri Mkaddem M, Zrig A, Ben Abdallah M, Romdhane M, Okla MK, Al-Hashimi A, AbdElgawad H. 2022. Variation of the chemical composition of essential oils and total phenols content in natural populations of Marrubium vulgare L. Plants, 11(5): 612.
  • Kacar B, İnal A. 2008. Plant analysis. Nobel Press, Ankara, Türkiye, pp: 138.
  • Knoss W. 1999. Marrubium vulgare (white horehound): In vitro culture, and the production of diterpene marrubiin and other secondary metabolites. In: Bajaj YPS, ed. Medicinal and Aromatic Plants XI. Springer, Berlin/Heidelberg, Almanya, pp: 274-289.
  • Lodhi S, Vadnere GP, Sharma VK, Usman MR. 2017. Marrubium vulgare L.: A review on phytochemical and pharmacological aspects. J Intercult Ethnopharmacol, 6(4): 429-452.
  • Lutz M, Jorquera K, Cancino B, Ruby R, Henriquez C. 2011. Phenolics and antioxidant capacity of table grape (Vitis vinifera L.) cultivars grown in Chile. J Food Sci, 76: 1088-1093.
  • Maiti R, González Rodríguez H, Kumari A, Chandra Sarkar N. 2016. Macro and micro-nutrient contents of 18 medicinal plants used traditionally to alleviate diabetes in Nuevo Leon, northeast of Mexico. Pak J Bot, 48(1): 271-276.
  • Matkowski A, Piotrowska M. 2006. Antioxidant and free radical scavenging activities of some medicinal plants from the Lamiaceae. Fitoterapia, 77(5): 346-353.
  • Matkowski A, Tasarz P, Szypuła E. 2008. Antioxidant activity of herb extracts from five medicinal plants from Lamiaceae, subfamily Lamioideae. J Med Plant Res, 2(11): 321-330.
  • Michalak M, Stryjecka M, Zagórska-Dziok M, Żarnowiec P. 2024. Biological activity of horehound (Marrubium vulgare L.) herb grown in Poland and its phytochemical composition. Pharmaceuticals, 17(6): 780.
  • Michalak M. 2022. Plant-derived antioxidants: Significance in skin health and the ageing process. Int J Mol Sci, 23: 585.
  • Nwozo OS, Effiong EM, Aja PM, Awuchi CG. 2023. Antioxidant, phytochemical, and therapeutic properties of medicinal plants: a review. Int J Food Prop, 26(1): 359-388.
  • Obanda M, Owuor PO, Taylor SJ. 1997. Flavanol composition and caffeine content of green leaf as quality potential indicators of Kenyan black teas. J Sci Food Agric, 74(2): 209-215.
  • Quettier-Deleu C, Gressier B, Vasseur J, Dine T, Brunet C, Luyckx M, Trotin F. 2000. Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. J Ethnopharmacol, 72(1–2): 35-42.
  • Rezgui M, Basma M, Neng N, Nogueira JM, Bettaieb Ben-Kaab L, Machado Araújo ME. 2021. Evaluation of Marrubium vulgare growing wild in Tunisia for its potential as a dietary supplement. Foods, 10(11): 2864.
  • Sekeroglu N, Ozkutlu F, Kara SM, Ozguven M. 2008. Determination of cadmium and selected micronutrients in commonly used and traded medicinal plants in Turkey. J Sci Food Agric, 88(1): 86-90.
  • Shallari S, Schwartz C, Hasko A, Morel JL. 1998. Heavy metals in soils and plants of serpentine and industrial sites of Albania. Sci Total Environ, 209(2–3): 133-142.
  • Tercan H, Ayanoglu F, Bahadirli NP. 2016. Determination of heavy metal contents and some basic aspects of widely used herbal teas in Turkey. Nature, 3(4): 1019-1022.
  • VanderJagt TJ, Ghattas R, VanderJagt DJ, Crossey M, Glew RH. 2002. Comparison of the total antioxidant content of 30 widely used medicinal plants of New Mexico. Life Sci, 70(9): 1035-1040.
  • Wojdyło A, Oszmiański J, Czemerys R. 2007. Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chem, 105: 940-949.
Toplam 30 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Ziraat Mühendisliği (Diğer)
Bölüm Araştırma Makalesi
Yazarlar

Lütfi Nohutçu 0000-0003-2250-2645

Gönderilme Tarihi 18 Mayıs 2025
Kabul Tarihi 2 Temmuz 2025
Erken Görünüm Tarihi 12 Temmuz 2025
Yayımlanma Tarihi 15 Temmuz 2025
Yayımlandığı Sayı Yıl 2025 Cilt: 8 Sayı: 4

Kaynak Göster

APA Nohutçu, L. (2025). Characterization of the Elemental Constituents, Phenolic Profile, and Antioxidant Capacity of Cultivated Horehound (Marrubium vulgare L.) Leaves from Van, Türkiye. Black Sea Journal of Agriculture, 8(4), 505-509. https://doi.org/10.47115/bsagriculture.1701538

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