Calculus bovis Ekstraktının Antioksidan, Sitotoksik, Apoptotik/Nekrotik ve Gen Ekspresyon Düzeylerinin Belirlenmesi

Yıl 2025, Cilt: 15 Sayı: 1, 1 - 20

Semih Dalkılıç , Lütfiye Kadıoğlu Dalkılıç , Ayşenur Çil , Gökhan Akay , İsmail Korkmaz , Songül Fidan

https://doi.org/10.55024/buyasambid.1665035

Öz

Kanser, her yıl milyonlarca insanın ölümüne neden olmakta ve tüm insanlığı etkilemektedir. 2030 yılına kadar 22 milyon yeni vaka öngörülmektedir. Standart kemoterapötik ajanların yan etkileri ve tedavi sonrası tümör tekrarı, doğal ve yenilebilir ürünlerden elde edilen moleküllerin geliştirilmesine yönlendirmiştir. Bu çalışmada, Calculus bovis ekstraktlarının kanser hücreleri üzerindeki etkileri incelenmiştir. MDA-MB-231, A549, PANC-1 ve OVCAR-3 hücre hatlarında MTT testi ile sitotoksik aktivite değerlendirilmiştir. Apoptotik/nekrotik etki çift boyama yöntemi ile analiz edilmiştir. Bax, Bcl-2, p21 ve p53 gen ekspresyon seviyeleri OVCAR-3 hücre hattında metanol ektraktında qRT-PCR yöntemiyle ölçülmüştür. Elde edilen sonuçlar gösteriyor ki aseton ekstraktı, OVCAR-3 hücre hattında belirgin sitotoksik etki göstermiştir. Aynı hücre hattında metanol ektraktında Bcl-2 gen ekspresyonunun baskılandığı ve apoptozu tetiklediği belirlenmiştir. Bulgular, Calculus bovis’in özellikle over ve meme kanseri tedavisi için potansiyel bir kemoterapötik ajan olabileceğini göstermektedir. Ekstraktın farklı kanser hücre hatlarına karşı yan etki göstermeden etkili olacağını umuyoruz. Ancak, yan etki profili henüz belirlenmediğinden araştırması gerekmektedir.

Anahtar Kelimeler

Sitotoksisite , Apoptoz , Nekroz , Antioksidan , Gen ekspresyonu

Proje Numarası

1919B012001124

Determination of Antioxidant, Cytotoxic, Apoptotic/Necrotic and Gene Expression Levels of Calculus bovis Extract

Öz

Cancer kills millions of people every year and affects all of humanity. 22 million new cases are projected by 2030. Side effects of standard chemotherapeutic agents and tumor recurrence after treatment have led to the development of molecules derived from natural and edible products. In this study, the impact of Calculus bovis extracts on cancer cells was examined. In MDA-MB-231, A549, PANC-1, and OVCAR-3 cell lines, cytotoxic activity was evaluated by MTT assay. The apoptotic/necrotic effect was analyzed by the double staining method. Bax, Bcl-2, p21, and p53 gene expression levels were measured by qRT-PCR in methanol extract in the OVCAR-3 cell line. The results showed that acetone extract showed a significant cytotoxic effect in the OVCAR-3 cell line. In the same cell line, the methanol extract suppressed Bcl-2 gene expression and triggered apoptosis. The findings suggest that Calculus bovis may be a potential chemotherapeutic agent, especially for the treatment of ovarian and breast cancer. We expect the extract to be effective against different cancer cell lines without side effects. However, its side effect profile is yet to be determined and needs further research.

Anahtar Kelimeler

Cytotoxicity , Apoptosis , Necrosis , Antioxidant , Gene expression

Etik Beyan

Our study did not involve any procedures requiring ethical approval; therefore, ethical approval was not obtained.

Destekleyen Kurum

The authors declared that they did not receive funding for this study and that there was no private economic interest in conducting the research.

Proje Numarası

1919B012001124

Teşekkür

We are grateful to all researchers. This research was part of PhD Project, which is supported by The Scientific and Technological Research Council of Turkey (TUBITAK) 2209-A Program, project no:1919B012001124. We would like to thank Prof. Dr. Mustafa Kaplan for his support and the opportunity to use the laboratory facilities of Firat University Faculty of Medicine, Department of Parasitology in this study.

Kaynakça

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