Acrolein-induced histopathological alterations in the liver of goldfish, Carassius auratus (Linnaeus, 1758)

Year 2018, Volume: 15 Issue: 2, 63 - 75, 30.11.2018

Sezgi Arman , Sema İşisağ Üçüncü

Abstract

The present study was conducted to examine the
potential histopathological changes caused by a herbicide, acrolein, in the
liver of Carassius auratus (goldfish).
Fish were exposed to 1, 5 and 25 µg/L acrolein for 96 h. Liver tissues were
removed, fixed with Bouin’s fluid and embedded in paraffin. 5 µm serial
sections were stained with hematoxylin eosin and the samples were investigated
by light microscopy. Acrolein treatment gave rise to sinusoidal dilatation and
congestion, vacuolar degeneration, hemorrhage, lymphocyte infiltration,
presence of enlarged melanomacrophagic centers, cloudy swelling, nucleolus
absence, and necrosis. These results are important for paying attention to
acrolein usage limits and its contamination in the aquatic environment.

Keywords

Acrolein, Carassius auratus, goldfish, herbicide, histopathology, liver

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