Meme Kanseri Proteom Profilinin Yüzey Geliştirilmiş Lazer Desorpsiyon / İyonizasyon Uçuş Kütle Spektrometrisi (SELDI-TOF MS) ile Belirlenmesi

Yasemin Baskın; Türkan Yiğitbaşı; Murat Kemal Atahan; Hasan Küpeli; Seyran Yiğit; Ercüment Tarcan

Yıl 2015, Cilt: 2 Sayı: 4, 101 - 105, 11.12.2015

Yasemin Baskın Türkan Yiğitbaşı Murat Kemal Atahan Hasan Küpeli Seyran Yiğit Ercüment Tarcan

Öz

Objective: Surface-enhanced laser desorption/ionization time-of flight mass spectrometry (SELDI-TOF MS) was used to screen serum proteomic
profiling to identify protein markers for early breast cancer patients.
Methods: We used metal ion affinity chromatography (IMAC30-Cu) for serum proteome fractionation technique and identify differentially
expressed peptides (m/z; P < 0.0001) from benign disease and healthy controls as potential biomarkers of early breast cancer. The mass spectra,
analyzed using ProteinChip Data Manager Software, distinguished between patients and healthy individuals based on k-nearest neighbor genetic
algorithm. We collected 70 serum samples from patients with breast cancer (62 invasive breast carcinoma; 8 ductal carcinoma in situ), 31 bening
disease and 37 healthy individuals.
Results: Forty two peaks showed statistically significantly different intensities between cases and controls (P<0.05); 10 protein peaks were
differentially expressed between the patients and benign disease. These results indicate that there are differences in serum protein expression among
the three different groups of patients.
Conclusion: SELDI-TOF MS could be used to screen blood samples for the early detection of breast cancer patients. Serum proteomic profiling by
SELDI-TOF MS is a novel potential tool for the clinical diagnosis of patients with breast carcinoma.

Anahtar Kelimeler

serum proteomic profiling, SELDI-TOF MS, Breast carcinoma

Kaynakça

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