Detection of specific microorganism in infected root canals of patients with Diabetes Mellitus using PCR

Sevinç Aktemur Türker; Gülhan Avci; Füsun Cömert

doi:10.65022/clindentres.1725487

Detection of specific microorganism in infected root canals of patients with Diabetes Mellitus using PCR

Abstract

Background and Aim: The aim of this in vitro study was to evaluate the presence of Enterococcus faecalis, Prevotella intermedia, Fusobacterium nucleatum, Porphyromonas gingivalis, Peptostreptococcus micros, Streptococcus spp., and Candida albicans in the primary and secondary/persistent infected root canals of patients with diabetes mellitus by polymerase chain reaction (PCR). Materials and Methods: Microbial samples were collected from 30 root canals of 30 patients with type 2 diabetes, who had either primary (n=15) or secondary/persistent (n=15) root canal infections. PCR was used to detect 6 bacterial and 1 fungal species. DNA extracted from the samples was analysed for the presence of 16S rDNA and then for the target species by species-specific primers. Qualitative variables were statistically analysed via the Pearson chisquare test, Yates-corrected chi-square test, and Fisher’s exact test. The difference between groups in terms of age was analysed via the independent samples t-test (p = 0.05). Results: 16S rDNA was detected in all the samples. Twentyone samples produced amplicons with 1 or more of the 6 specific bacterial primer pairs. No amplicons with any of the specific primers were observed in the 9 samples where 16S rDNA was detected. All the microorganisms tested were detected with equal prevalence in both primary and secondary/persistent root canal infections (p>0.05). Streptococcus spp. was the most frequently detected species in primary (53.3%) and secondary/ persistent (46.7%) infections. C. albicans was not detected in any of the samples. E. faecalis was detected in only one sample of secondary/persistent infections. Conclusion: Primary and secondary/persistent root canal infections in patients with diabetes mellitus had similar microbial compositions.

Keywords

Supporting Institution

This study was supported by Zonguldak Bülent Ecevit University Scientific Research Projects Coordination Office with the project number

Project Number

2020-34876299-02

Ethical Statement

This study protocol was approved by the Clinical Research Ethics Committee of the University of Zonguldak Bülent Ecevit University (approval number: 2020/19).

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Details

Primary Language

English

Subjects

Endodontics

Journal Section

Research Article

Authors

Sevinç Aktemur Türker ^*
0000-0001-8740-2480
Türkiye

Gülhan Avci
0000-0001-7426-0567
Türkiye

Füsun Cömert
0000-0003-0161-6897
Türkiye

Publication Date

April 29, 2026

Submission Date

June 23, 2025

Acceptance Date

September 11, 2025

Published in Issue

Year 2026 Volume: 50 Number: 1

DOI

https://doi.org/10.65022/clindentres.1725487

IZ

https://izlik.org/JA96HP65MR

Cite

RIS / Bibtex

APA

Aktemur Türker, S., Avci, G., & Cömert, F. (2026). Detection of specific microorganism in infected root canals of patients with Diabetes Mellitus using PCR. Clinical Dentistry and Research, 50(1), 30-37. https://doi.org/10.65022/clindentres.1725487

AMA

1.Aktemur Türker S, Avci G, Cömert F. Detection of specific microorganism in infected root canals of patients with Diabetes Mellitus using PCR. Clin Dent Res. 2026;50(1):30-37. doi:10.65022/clindentres.1725487

Chicago

Aktemur Türker, Sevinç, Gülhan Avci, and Füsun Cömert. 2026. “Detection of Specific Microorganism in Infected Root Canals of Patients With Diabetes Mellitus Using PCR”. Clinical Dentistry and Research 50 (1): 30-37. https://doi.org/10.65022/clindentres.1725487.

EndNote

Aktemur Türker S, Avci G, Cömert F (April 1, 2026) Detection of specific microorganism in infected root canals of patients with Diabetes Mellitus using PCR. Clinical Dentistry and Research 50 1 30–37.

IEEE

[1]S. Aktemur Türker, G. Avci, and F. Cömert, “Detection of specific microorganism in infected root canals of patients with Diabetes Mellitus using PCR”, Clin Dent Res, vol. 50, no. 1, pp. 30–37, Apr. 2026, doi: 10.65022/clindentres.1725487.

ISNAD

Aktemur Türker, Sevinç - Avci, Gülhan - Cömert, Füsun. “Detection of Specific Microorganism in Infected Root Canals of Patients With Diabetes Mellitus Using PCR”. Clinical Dentistry and Research 50/1 (April 1, 2026): 30-37. https://doi.org/10.65022/clindentres.1725487.

JAMA

1.Aktemur Türker S, Avci G, Cömert F. Detection of specific microorganism in infected root canals of patients with Diabetes Mellitus using PCR. Clin Dent Res. 2026;50:30–37.

MLA

Aktemur Türker, Sevinç, et al. “Detection of Specific Microorganism in Infected Root Canals of Patients With Diabetes Mellitus Using PCR”. Clinical Dentistry and Research, vol. 50, no. 1, Apr. 2026, pp. 30-37, doi:10.65022/clindentres.1725487.

Vancouver

1.Sevinç Aktemur Türker, Gülhan Avci, Füsun Cömert. Detection of specific microorganism in infected root canals of patients with Diabetes Mellitus using PCR. Clin Dent Res. 2026 Apr. 1;50(1):30-7. doi:10.65022/clindentres.1725487