STEREOMICROSCOPIC EVALUATION OF PRE-STERILIZATION METHODS EFFICIENCY IN REMOVAL OF CALCIUM HYDROXIDE FROM THE SURFACE OF ENDODONTIC INSTRUMENTS
Year 2024,
Volume: 48 Issue: 1
,
21
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29
,
30.04.2024
Emel Uzunoğlu Özyürek
,
Selen Küçükkaya Eren
Abstract
Background and Aim: This study aimed to evaluate the amount of calcium hydroxide (CH) remnants on nickel-titanium rotary instruments after applying different cleaning methods.
Materials and Methods: Fifty ProTaper Gold F2 and 50 ProTaper Next X2 instruments were used, and ten instruments from each file system served as a negative control. The surfaces of the remaining instruments were covered with CH, and ten instruments from each file system were assigned to the positive control group. The instruments in the experimental groups were cleaned with one of the following methods (n=10): Group SB: Instruments were cleaned with a sponge and brushed manually. Group SU: Instruments were cleaned with a sponge and agitated in an ultrasonic bath. Group SBU: Instruments were cleaned with a sponge, brushed manually, and agitated in an ultrasonic bath. After autoclaving, the instruments were examined under a stereomicroscope. The amount of CH remnants in the instruments’ apical and coronal cutting parts were scored. Data was analyzed statistically at p-value <0.05.
Results: The amount of CH remnants was lower on the surface of ProTaper Gold F2 compared to ProTaper Next X2 in Group SB (p=0.029 in the apical part, p=0.013 in the coronal part). Additional use of ultrasonic bath caused significant improvement in the removal of CH from the instrument surface for both file systems (p=0.002). The scores of the apical and the coronal parts were similar in all groups (p=0.157 for ProTaper Gold F2 and p=0.317 for ProTaper Next X2).
Conclusion: Using an ultrasonic bath before autoclaving can be recommended to improve instrument cleaning.
References
-
1. Sonntag D, Martin E, Raab WH. Representative survey on the
reprocessing of endodontic instruments in Germany. Br Dent J
2016; 220: 465-469.
-
2. Vassey M, Budge C, Poolman T, Jones P, Perrett D, Nayuni N et
al. A quantitative assessment of residual protein levels on dental
instruments reprocessed by manual, ultrasonic and automated
cleaning methods. Br Dent J 2011; 210: 14.
-
3. Linsuwanont P, Parashos P, Messer HH. Cleaning of rotary nickeltitanium
endodontic instruments. Int Endod J 2004; 37: 19-28.
-
4. Aasim SA, Mellor AC, Qualtrough AJ. The effect of pre-soaking
and time in the ultrasonic cleaner on the cleanliness of sterilized
endodontic files. Int Endod J 2006; 39: 143-149.
-
5. Chan HWA, Tan KH, Dashper SG, Reynolds EC, Parashos P.
Sterilization of rotary NiTi instruments within endodontic sponges.
Int Endod J 2016; 49: 850-857.
-
6. Popovic J, Gasic J, Zivkovic S, Petrovic A, Radicevic G. Evaluation
of biological debris on endodontic instruments after cleaning and
sterilization procedures. Int Endod J 2010; 43: 336-341.
-
7. Gavini G, Santos MD, Caldeira CL, Machado MEL, Freire LG,
Iglecias EF et al. Nickel-titanium instruments in endodontics: a
concise review of the state of the art. Braz Oral Res 2018; 32: 67.
-
8. Mohammadi Z, Dummer PM. Properties and applications of
calcium hydroxide in endodontics and dental traumatology. Int
Endod J 2011; 44: 697-730.
-
9. Ghabraei S, Bolhari B, Yaghoobnejad F, Meraji N. Effect of intracanal
calcium hydroxide remnants on the push-out bond strength
of two endodontic sealers. Iran Endod J 2017; 12: 168-172.
-
10. Contardo L, De Luca M, Bevilacqua L, Breschi L, Di Lenarda R.
Influence of calcium hydroxide debris on the quality of endodontic
apical seal. Minerva Stomatol 2007; 56: 509-517.
-
11. Tamil S, Andamuthu SA, Vaiyapuri R, Prasad AS, Jambai SS,
Chittrarasu M. A Comparative Evaluation of Intracanal Calcium
Hydroxide Removal with Hand File, Rotary File, and Passive
Ultrasonic Irrigation: An In Vitro Study. J Pharm Bioallied Sci 2019;
11: 442-445.
-
12. Kenee DM, Allemang JD, Johnson JD, Hellstein J, Nichol BK. A
quantitative assessment of efficacy of various calcium hydroxide
removal techniques. J Endod 2006; 32: 563-565.
-
13. Filho MT, Leonardo MR, Bonifácio KC, Dametto FR, Silva LAB.
The use of ultrasound for cleaning the surface of stainless steel
and nickel–titanium endodontic instruments. Int Endod J 2001; 34:
581-585.
-
14. Parashos P, Linsuwanont P, Messer HH. A cleaning protocol for
rotary nickel-titanium endodontic instruments. Aust Dent J 2004;
49: 20-27.
-
15. Van Eldik DA, Zilm PS, Rogers AH, Marin PD. A SEM evaluation
of debris removal from endodontic files after cleaning and steam
sterilization procedures. Aust Dent J 2004; 49: 128-135.
-
16. Parirokh M, Kazemizadeh Z, Shahravan A, Sahranavard G,
Haghdoost AA. A comparative study of the cleaning effect
of various ultrasonic cleaners on new, unused endodontic
instruments. J Oral Health Oral Epidemiol 2014; 3: 85-91.
-
17. Sonntag D, Peters OA. Effect of prion decontamination
protocols on nickel-titanium rotary surfaces. J Endod 2007; 33:
442-446.
-
18. Letters S, Smith AJ, McHugh S, Bagg J. A study of visual and
blood contamination on reprocessed endodontic files from general
dental practice. Br Dent J 2005; 199: 522-525.
-
19. Bagg J, Sweeney CP, Roy KM, Sharp T, Smith A. Cross infection control measures and the treatment of patients at risk of
Creutzfeldt Jakob disease in UK general dental practice. Br Dent J
2001; 191: 87-90.
-
20. Dioguardi M, Arena C, Sovereto D, Aiuto R, Laino L, Illuzzi G et al.
Influence of sterilization procedures on the physical and mechanical
properties of rotating endodontic instruments: a systematic review.
-
21. Dioguardi M, Laneve E, Di Cosola M, Cazzolla AP, Sovereto D,
Aiuto R et al. The Effects of Sterilization Procedures on the Cutting
Efficiency of Endodontic Instruments: A Systematic Review and
Network Meta-Analysis. Materials (Basel) 2021; 14: 1559.
-
22. Smith A, Dickson M, Aitken J, Bagg J. Contaminated dental
instruments. J Hosp Infect 2002; 51: 233-235.
-
23. Gurevich I, Dubin R, Cunha BA. Dental instrument and device
sterilization and disinfection practices. J Hosp Infect 1996; 32:
295-304.
-
24. Ramakrishna SP, Ramchandra MW, Sachin MC, AnupKiran M,
Singh H. To ascertain effectiveness of pre-sterilization cleaning of
endodontic instruments before placement in glass bead sterilizer–
An in vitro study. IP Indian J Conserv Endod 2016; 1: 42-46.
-
25. McDonnell G, Russell AD. Antiseptics and disinfectants:
activity, action, and resistance. Clin Microbiol Rev 1999; 12: 147-
179.
-
26. Gilbert P, Al-Hiti MM, Smith PM. A laboratory evaluation of a
new disinfectant cleaner for fibreoptic instruments. Microbios
1982; 33: 53-64.
-
27. Bagg J, Smith AJ, Hurrell D, McHugh S, Irvine G. Pre-sterilisation
cleaning of re-usable instruments in general dental practice. Br
Dent J 2007; 202: 22; discussion 550-551.
-
28. Walker JT, Dickinson J, Sutton JM, Raven ND, Marsh PD.
Cleanability of dental instruments--implications of residual protein
and risks from Creutzfeldt-Jakob disease. Br Dent J 2007; 203:
395-401.
-
29. Elmsallati EA, Wadachi R, Ebrahim AK, Suda H. Debris retention
and wear in three different nickel-titanium rotary instruments.
Aust Endod J 2006; 32: 107-111.
-
30. Gagliardi J, Versiani MA, de Sousa-Neto MD, Plazas-Garzon A,
Basrani B. Evaluation of the Shaping Characteristics of ProTaper
Gold, ProTaper NEXT, and ProTaper Universal in Curved Canals. J
Endod 2015; 41: 1718-1724.
-
31. Bird DC, Chambers D, Peters OA. Usage parameters of nickeltitanium
rotary instruments: a survey of endodontists in the United
States. J Endod 2009; 35: 1193-1197.
-
32. Enabulele JE, Omo JO. Sterilization in endodontics: Knowledge,
attitude, and practice of dental assistants in training in Nigeria–A
cross-sectional study. Saudi Endod J 2018; 8: 106-110.
-
33. Cayo-Rojas CF, Brito-Ávila E, Aliaga-Mariñas AS, Hernández-
Caba KK, Saenz-Cazorla ED, Ladera-Castañeda MI, et al. Cleaning
of endodontic files with and without enzymatic detergent by
means of the manual method versus the ultrasonic method: An
experimental study. J Int Soc Prev Community Dent 2021; 11: 307-
315.
-
34. Bryson LM, Fernandez Rivas D, Boutsioukis C. Cleaning of
used rotary nickel-titanium files in an ultrasonic bath by locally
intensified acoustic cavitation. Int Endod J 2018; 51: 457-468.
-
35. Perakaki K, Mellor A, Qualtrough A. Comparison of an ultrasonic
cleaner and a washer disinfector in the cleaning of endodontic
files. J Hosp Infect 2007; 67: 355-359.