Cytotoxicity of Sambucus nigra L. on Cancer Cell Line and In Vitro Antioxidant Properties

Year 2023, , 896 - 901, 29.12.2023

Gül Akduman , Serol Korkmaz , Turgut Taşkın , Fatma Esra Güneş

https://doi.org/10.33808/clinexphealthsci.1190301

Cited By: 1

Abstract

Objective: Essential oils, free fatty acids, flavonoids, glycosides, phenolic acids, carotenoids, vitamins, and minerals are found in significant quantities in the characteristic chemical composition of Sambucus nigra L. This study aimed to evaluate the antioxidant potential of Sambucus nigra L. Fructus and evaluate the cytotoxicity on the cancer cell line.
Methods: The Sambucus nigra L. fruits were collected from Yalova Atatürk Horticultural Central Research Institute in September 2021. The ethanol extract was prepared. Antioxidant property of Sambucus nigra L. fruit extracts was evaluated with 2,2-diphenyl-1-picryl-hydrazyl- hydrate (DPPH) radical scavenging activity, Cupric Ion Reducing Antioxidant Capacity (CUPRAC). Also, total phenolic content, total flavonoid content, and total anthocyanin content were calculated. Liver hepatocellular carcinoma cell line (HepG2) was used for cytotoxicity assay and an 2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was applied.
Results: The total phenolic, total flavonoid contents and total monomeric anthocyanins were 9.75±0.92 mg GAE/ mg fruit extract, 0.07437±0.004 mg quercetin/ mg fruit extract, respectively. 0.45±0.0014 mg catechin/gram of fruit extract and 2.08±0.025 mg Cyanidin-3 glucosides/g fruit extract. CUPRAC and DPPH results showed that Sambucus nigra L. extract has strong antioxidant activity. The results of the cytotoxicity assay indicated that while concentrations of the extract increased, the viability of HepG2 decreased.
Conclusion: Our findings suggest that the Sambucus fruit extract is particularly rich in antioxidant components that are possibly modulating their beneficial use for hepatocellular malignancies, significantly reducing the number of viable cancer cells and inducing cell death.

Keywords

Sambucus nigra, Elderberry, Cytotoxicity, Antioxidants

References

• Młynarczyk K., Walkowiak-Tomczak D., Łysiak GP. Bioactive properties of Sambucus nigra L. As a functional ingredient for food and pharmaceutical industry. J Funct. Foods. 2018;40:377–390. DOI: 10.1016/j.jff.2017.11.025
• Lee J, Finn CE. Anthocyanins and other polyphenolics in American elderberry (Sambucus canadensis) and European elderberry (S. nigra) cultivars. J Sci Food Agric. 2007;87:2665–2675. DOI: 10.1002/jsfa.3029
• Paredes-López O, Cervantes-Ceja ML, Vigna-Pérez M, Hernández- Pérez T. Berries: improving human health and healthy aging, and promoting quality life – a review. Plant Foods Hum Nutr. 2010;65:299–308. DOI: 10.1007/s11130-010-0177-1
• Neves D, Valentão P, Bernardo J, Oliveira MC, Ferreira JMG, Pereira DM, Andrade PB, Videira RA. A new insight on elderberry anthocyanins bioactivity: Modulation of mitochondrial redox chain functionality and cell redox state. J Funct Foods. 2019;56:145–155. DOI: 10.1016/j.jff.2019.03.019
• Duymuş HG, Göger F, Başer KHC. In vitro antioxidant properties and anthocyanin compositions of elderberry extracts. Food Chem. 2014;155:112–119. DOI: 10.1016/j.foodchem.2014.01.028
• Muraina IA, Suleiman MM, Eloff JN. Can MTT be used to quantify the antioxidant activity of plant extracts?. Phytomedicine. 2009;16(6-7):665-668. DOI: 10.1016/j.phymed.2008.11.005
• Liu Y, Nair MG. An efficient and economical MTT assay for determining the antioxidant activity of plant natural product extracts and pure compounds. J. Nat. Prod. 2010;73(7):1193-1195. DOI: 10.1021/np1000945
• Apak R, Güclü K, Ozyurek M, Karademir SE. Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC Method. J Agric Food Chem. 2004;52(26):7970–7981. DOI: 10.1021/jf048741x
• Taskin T, Balkan IA, Taskın D, Dogan A. Characterization of phenolic constituents and pharmacological activity of Achillea vermicularis. Indian J Pharm Sci. 2019;81(2):293–301. DOI: 10.36468/pharmaceutical-sciences.
• Majkic TM, Torovic LD, Lesjak MM, Cetojevic-Simin DD, Beara IN. Activity profiling of Serbian and some other European Merlot wines in inflammation and oxidation processes. Food Res Int. 2019;121: 51–160. DOI: 10.1016/j.foodres.2019.03.033
• Tungmunnithum D, Thongboonyou A, Pholboon A, Yangsabai A. Flavonoids and Other Phenolic Compounds from Medicinal Plants for Pharmaceutical and Medical Aspects: An Overview. Medicines. 2018;5(3): 93. DOI: 10.3390/medicines5030093
• Atkinson MD, Atkinson E. Sambucus nigra L. J. Ecol. 2002;90:895-923. DOI: 10.1046/j.1365-2745.2002.00698.x
• Yıldız Ö, Vahapoğlu B, Marangoz MA, Güven EÇ, Bayindirli A. Determination of Phenolic Compound Profiles and Antioxidant Effect of Plant Extracts on Late-Release Soft Lozenge. EAS J Nutr Food Sci. 2021;3(6):167-174. DOI: 10.36349/easjnfs.2021.v03i06.005.
• Ercisli S, Tosun M, Akbulut M. Physico-chemical characteristics of some wild grown European Elderberry (Sambucus nigra L.) genotypes. Pharmacogn. Mag. 2009;5(20):320. DOI:10.4103/0973-1296.58153
• Özgen M, Scheerens JC, Reese RN, Miller RA. Total phenolic, anthocyanin contents and antioxidant capacity of selected elderberry (Sambucus canadensis L.) accessions. Pharmacogn Mag. 2010;6(23):198. DOI: 10.4103/0973-1296.66936
• Csorba V, Magdolna TÓTH., Laszlo AM, Kardos L, Kovacs S. Cultivar and year effects on the chemical composition of elderberry (Sambucus nigra L.) fruits. Not Bot Horti Agrobot Cluj Napoca. 2020;48(2):770-782. DOI:10.15835/nbha48211873
• Goud NS, Prasad G. Antioxidant, antimicrobial activity and total phenol and flavonoids analysis of Sambucus nigra (elderberry). Int J Curr Pharm Res. 2020;12(1):35-37. DOI: 10.22159/ijcpr.2020v12i1.36829.
• Moyer RA, Hummer KE, Finn CE, Frei B, Wrolstad RE. Anthocyanins, phenolics, and antioxidant capacity in diverse small fruits: vaccinium, rubus, and ribes. J Agric Food Chem. 2002;50(3):519-25. DOI: 10.1021/jf011062r.
• Veberic R, Jakopic J, Stampar F, Schmitzer V. European elderberry (Sambucus nigra L.) rich in sugars, organic acids, anthocyanins and selected polyphenols. Food Chem. 2009;114(2):511-515. DOI: 10.1016/j.foodchem.2008.09.080.
• Domínguez R, Zhang L, Rocchetti G, Lucini L, Pateiro M. Munekata PE, Lorenzo JM. Elderberry (Sambucus nigra L.) as potential source of antioxidants. Characterization, optimization of extraction parameters and bioactive properties. Food Chem. 2020;330: 127266. DOI: 10.1016/j.foodchem.2020.127266
• Walkowiak-Tomczak D, Czapski J, Młynarczyk K. Assessment of colour changes during storage of elderberry juice concentrate solutions using the optimization method. Acta Sci Pol Technol Aliment. 2016;15(3):299–309. DOI: 10.17306/J.AFS.2016.3.29
• Bahorun T, Luximon‐Ramma A, Crozier A, Aruoma OI. Total phenol, flavonoid, proanthocyanidin and vitamin C levels and antioxidant activities of Mauritian vegetables. J Sci Food Agric. 2004;84(12):1553-1561. DOI: 10.1002/jsfa.1820.
• Jakobek L, Šeruga M, Novak I, Medvidović-Kosanović M, Šeruga B. DPPH radical inhibition kinetic and antiradical activity of polyphenols from chokeberry and elderberry fruits. Pomologia Croatica: Glasilo Hrvatskog agronomskog društva, 2008;14(2):101-118.
• Has IM, Teleky BE, Szabo K, Simon E, Ranga F, Diaconeasa ZM, Nițescu M. Bioactive Potential of Elderberry (Sambucus nigra L.): Antioxidant, Antimicrobial Activity, Bioaccessibility and Prebiotic Potential. Molecules, 2023;28(7):3099. DOI: 10.3390/molecules28073099
• Poráčová J, Sedlak V, Pošiváková T, Mirutenko V, Gruľová D, Mydlárová-Blaščáková M, Kotosova J. Measurement of antioxidant activity in chokeberry (Aronia melanocarpa WILD.) and black elderberry (Sambucus nigra L.) using the DPPH method. İkinci Uluslararası Bilimsel ve Pratik İnternet Konferansı "Tıbbi Bitki Yetiştiriciliği: Geçmiş Deneyimlerden Modern Teknolojilere" Bildirileri, 2013;161:131. DOI: 10.1055/s-0032-1321041
• Pliszka B. Polyphenolic content, antiradical activity, stability and microbiological quality of elderberry (Sambucus nigra L.) extracts. Acta Sci. Pol. Technol. 2017;16(4):393-401. DOI: 10.17306/J.AFS.0523
• Esin Çelik S, Özyürek M, Güçlü K, Çapanoğlu E, Apak R. Identification and Anti‐oxidant Capacity Determination of Phenolics and their Glycosides in Elderflower by On‐line HPLC–CUPRAC Method. Phytochem. Anal. 2014;25(2):147-154. DOI: 10.1002/pca.2481
• Saravi SS, Shokrzadeh M, Shirazi FH. Cytotoxicity of Sambucus ebulus on cancer cell lines and protective effects of vitamins C and E against its cytotoxicity on normal cell lines. (2013). Afr J Biotechnol. 2013;12(21): 3360-3365. DOI: 10.5897/AJB09.1577
• Ferreira SS, Martins-Gomes C, Nunes FM, Silva AM. Elderberry (Sambucus nigra L.) extracts promote anti-inflammatory and cellular antioxidant activity. Food Chem. 2022;15:100437. DOI: 10.1016/j.fochx.2022.100437