Effects of melatonin on the tissue factor activities of kidney, heart and brain in experimental renovascular hypertension

Year 2012, Volume: 2 Issue: 2, 78 - 83, 31.01.2014

Ebru Alturfan , Ayşen Yarat Göksel Şener

Abstract

Objective: The aim of the present study was to investigate the effect of melatonin on tissue factor activities (TFa) of kidney, heart and brain in an angiotensin II-dependent renovascular hypertension (RVH) in rats.
Methods: RHV was induced in Wistar albino rats by placing renal artery clip (two-kidney, one-clip; 2K1C). Starting on the operation day (RHV-Mel-1) and 3 weeks after the operation (RHV-Mel-2), the rats received melatonin (10 mg/kg/day) while the control group received 1 mL/kg/day saline with 1% alcohol for the following 6 weeks. Indirect blood pressure (BP) measurement was made by the tail-cuff method. At the end of the 9th week, after BP recordings, kidney, heart and brain tissues were excised and stored at -200C until the analysis of TFa. TFa of tissues were determined according to Quick’s one stage method and results are expressed as seconds.
Results: 2K1C caused increase in BP and TFa of the kidney significantly but did not change TFa of heart and brain compared with the control group. In RVH-Mel-1 group, TFa of kidney decreased compared with the RVH group. TFa of kidney increased in RVH-Mel-2 group compared with the control. On the other hand, the TFa of heart increased in both melatonin-treated RVH groups compared with the control group.
Conclusion: Increased TFa of kidney in RVH group might promote renal artery stenosis and thrombosis development. On the other hand, increased TFa of heart in melatonin treated groups might provide additional hemostatic protections to this vital organ in order to prevent from excessive bleeding.

Key words: Renovascular hypertension, melatonin, tissue factor, kidney, heart, brain, rat

Keywords

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Deneysel renovasküler hipertansiyonda melatoninin böbrek, kalp ve beyin doku faktörü aktiviteleri üzerine etkileri

Abstract

Amaç: Çalışmamızın amacı anjiotensin II bağımlı renovasküler hipertansiyon (RHV) oluşturulmuş sıçanların böbrek, kalp ve beyin dokularının doku faktörü aktivitesi (TFa) üzerine melatoninin etkilerini incelemektir.
Yöntem: RHV Wistar albino sıçanlarda renal arter klips (iki böbrek, bir klips, 2K1C) yerleştirerek oluşturulmuş, kontrol grubuna ise klips yerleştirilmemiştir. Operasyon gününde (RHV-Mel-1) veya operasyondan 3 hafta sonra başlayacak şekilde (RHV-Mel-2) sıçanlara 6 hafta boyunca melatonin (10 mg/kg/gün), kontrol grubuna ise 1 mL/kg/gün salin ile %1 alkol verilmiştir. İndirekt kan basıncı (BP) ölçümü “tail-cuff” metodu ile yapılmıştır. 9. haftanın sonunda BP ölçümleri yapılmıştır. Böbrek, kalp ve beyin dokuları çıkartılarak TFa ölçümlerine kadar -200C’de saklanmıştır. Dokuların TF aktiviteleri “Quick’s one stage” metoduna göre yapıldı ve sonuçlar saniye olarak verildi.
Bulgular: Oluşturulan 2K1C modeli BP’de ve böbrek TFa’da kontrolle karşılaştırıldığında anlamlı artışa neden oldu; ancak, kalp ve beyin TFa’yı etkilemedi. RVH-Mel-1 grubunda, böbrek TFa değeri, RHV grubuyla karşılaştırıldığında azaldı. Böbrek TFa değerlerinin RVH-Mel-2 grubunda kontrol grubuna göre artmış olduğu bulundu. Ancak kalp TFa değerleri kontrol grubuyla karşılaştırıldığında melatonin ile tedavi edilen her iki RHV grubunda arttı.
Sonuç: RHV grubunda artmış böbrek TFa renal arter stenozu ve tromboz gelişimine neden olabilir. Melatonin ile tedavi edilen gruplarda artmış kalp TFa değerleri ise bu hayati organı aşırı kanamadan koruyacak ilave bir hemostatik koruma sağlayabilir.

Anahtar Kelimeler : Renovasküler hipertansiyon, melatonin, doku faktörü, böbrek, kalp, beyin, sıçan

Keywords

Renovasküler hipertansiyon, melatonin, doku faktörü, böbrek, kalp, beyin, sıçan

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