In vitro testing for genotoxicity of indigoid dyes by comet assay

Yıl 2012, Cilt: 2 Sayı: 3, 108 - 112, 30.01.2014

Seher Karslı-çeppioğlu Türkan Yurdun

Öz

Objective: Indigo and indigoid dyes are natural dyes and have been known since Bronze Age (B.C. 2000). Nowadays, indigoid dyes are widely used in the industry of textile, cosmetic, food and medicine. The aim of this study was to estimate the DNA damage of indigotin, 6-bromo indigotin, indirubin and 6-bromo indirubin by in vitro alkaline single-cell gel electrophoresis (SCGE-Comet) in the peripheral lymphocytes. 

Methods: The cytotoxic effects of indigo and indigoid dyes were assessed by trypan blue exclusion. The cells were incubated with 10, 25, 50 μg/mL of the test substances for 30 min at 37°C. Comet assay was used to evaluate the genotoxic effect of test substances on human peripheral lymphocytes.

Results: Our results revealed that indigotin and 6-bromo indigotin increased the DNA migration in a dose-dependent manner. DNA damage was higher in cells that had been incubated with 50 μg/mL indigotin and 6-bromo indigotin (p<0.05). 

Conclusion: Our results indicate that indigo and indigoid dyes would be genotoxic at higher concentrations. It is probable that a genotoxic effect might occur due to the fact that the individuals who have worked with these dyestuffs, both in the past and today, used highly concentrated dyes.

Key words: Indigotin, 6-bromo indigotin, indirubin, 6-bromo indirubin, comet assay, DNA damage

Anahtar Kelimeler

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İndigoid boyaların genotoksik etkisinin in vitro comet tekniği ile değerlendirilmesi

Öz

Amaç: Doğal kaynaklı indigo ve indigoid boyalar; Bronz Çağı’ndan (M.Ö. 2000) beri bilinmektedir. Son dönemde ise indigoid boyalar sıklıkla tekstil, kozmetik, gıda ve ilaç endüstrisinde kullanılmaktadır. Bu çalışmanın amacı, in vitro alkali tek-hücre jel elektroforez (Comet) tekniği ile indigotin, 6-bromo indigotin, indirubin ve 6-bromo indirubin’in insan lenfositlerinde neden olabileceği olası DNA hasarını değerlendirmektir

Yöntemler: İndigo ve indigoid boyaların sitotoksik etkisi, tripan mavisi yöntemi ile incelendi. Hücreler test bileşiklerinin 10, 25, 50 μg/mL’lik konsantrasyonları ile 37°C’de 30 dak inkübasyona bırakıldı. Test bileşiklerinin insan periferal lenfositleri üzerine genotoksik etkilerini değerlendirmek amacı ile comet tekniği kullanıldı.

Bulgular: Bu çalışma ile indigotin ve 6-bromo indigotin’in doza bağlı olarak DNA göçünü artırdığı görüldü. İndigotin ve 6-bromo indigotin’in 50 μg/mL’lik konsantrasyonu ile inkübe edilen hücrelerde DNA hasarı artmıştır (p<0.05). 

Sonuç: Elde ettiğimiz sonuçlara göre, indigo ve indigoid boyaların yüksek konsantrasyonda genotoksik etkiye yol açabileceği ileri sürülebilir. Sonuç olarak, boyarmaddeler ile çalışan bireylerde yüksek konsantrasyonda boyaların genotoksik etkiye yol açabileceği göz önünde tutulmalıdır.

Anahtar Kelimeler : İndigotin, 6-bromo indigotin, indirubin, 6-bromo indirubin, comet tekniği, DNA hasarı

Anahtar Kelimeler

İndigotin, 6-bromo indigotin, indirubin, 6-bromo indirubin, comet tekniği, DNA hasarı

Kaynakça

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