Pro-Inflammatory Cytokine and Caspase-1 Responses to Pattern Recognition Receptor Activation of Neutrophils and Dendritic Cells in Behcet’s Disease

Filiz Türe Özdemir; Aysın Tulunay; Mehmet Onur Elbaşı; İmren Tatlı; Anne-Marie Maurer; Gonca Mumcu; Haner Direskeneli; Emel Ekşioğlu Demiralp

Pro-Inflammatory Cytokine and Caspase-1 Responses to Pattern Recognition Receptor Activation of Neutrophils and Dendritic Cells in Behcet’s Disease

Year 2012, Volume: 2 Issue: 6, 0 - , 31.01.2014

Filiz Türe Özdemir Aysın Tulunay Mehmet Onur Elbaşı İmren Tatlı Anne-Marie Maurer Gonca Mumcu Haner Direskeneli Emel Ekşioğlu Demiralp

Abstract

Objectives: Activated innate immunity is implicated in the pathogenesis of Behcet’s Disease (BD). To clarify the mechanisms of innate immune responses, we investigated inflammasome activation in dendritic cells (DC) and neutrophils, following stimulation with two different pattern recognition receptors (PRR) “RIG-1 like” (RLR) and “NOD-like” (NLR) in patients with BD.

Methods: Sixteen active BD patients with mucocutaneous lesions and 17 healthy controls (HC) were included in this study. Dendritic cells were generated from monocytes. DCs and isolated neutrophils were activated by RLR and NLR ligands. Caspase-1 activation and expressions of p38 and RIP2 were determined by flow cytometry. Levels of IL-1ß, IL-6, TNF-?, IFN-? and IL-18 in culture supernatants were measured by ELISA.

Results: Activation of caspase-1 following intracellular PRR stimulation was found to be in similar levels in DCs and neutrophils of BD patients compared to HC. However, activation of DCs from BD patients to NOD2 stimulus measured with the expressions of RIP2, p38 as well as IL-18 levels was found to be slightly defective (p<0.05). In neutrophil cultures, IL-6 levels were lower in response to all stimuli in patients with BD compared to HCs (p<0.01).

Conclusions: Inflammasome formation following stimulation with NOD1/NOD2 and RIG measured with caspase-1 activation, cytokine levels and expressions of RIP2 and p38 seems to be functionally normal in DCs and neutrophils of BD patients, although slightly defective responses in some pathways and cytokine levels were observed. These results may suggest that caspase-1 independent pathways such as TLRs may be more prominent in BD pathogenesis.

Anahtar Kelimeler : Behcet’s disease, dendritic cells, inflammasome, neutrophils, pattern-recognition receptors

Keywords

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Year 2012, Volume: 2 Issue: 6, 0 - , 31.01.2014

Filiz Türe Özdemir Aysın Tulunay Mehmet Onur Elbaşı İmren Tatlı Anne-Marie Maurer Gonca Mumcu Haner Direskeneli Emel Ekşioğlu Demiralp

Abstract

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Details

Primary Language	Turkish
Journal Section	Articles
Authors	Filiz Türe Özdemir This is me Aysın Tulunay This is me Mehmet Onur Elbaşı This is me İmren Tatlı This is me Anne-Marie Maurer This is me Gonca Mumcu This is me Haner Direskeneli This is me Emel Ekşioğlu Demiralp This is me
Publication Date	January 31, 2014
Submission Date	January 31, 2014
Published in Issue	Year 2012 Volume: 2 Issue: 6

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APA	Türe Özdemir, F., Tulunay, A., Elbaşı, M. O., Tatlı, İ., et al. (2014). Pro-Inflammatory Cytokine and Caspase-1 Responses to Pattern Recognition Receptor Activation of Neutrophils and Dendritic Cells in Behcet’s Disease. Clinical and Experimental Health Sciences, 2(6).
AMA	Türe Özdemir F, Tulunay A, Elbaşı MO, Tatlı İ, Maurer AM, Mumcu G, Direskeneli H, Ekşioğlu Demiralp E. Pro-Inflammatory Cytokine and Caspase-1 Responses to Pattern Recognition Receptor Activation of Neutrophils and Dendritic Cells in Behcet’s Disease. Clinical and Experimental Health Sciences. February 2014;2(6).
Chicago	Türe Özdemir, Filiz, Aysın Tulunay, Mehmet Onur Elbaşı, İmren Tatlı, Anne-Marie Maurer, Gonca Mumcu, Haner Direskeneli, and Emel Ekşioğlu Demiralp. “Pro-Inflammatory Cytokine and Caspase-1 Responses to Pattern Recognition Receptor Activation of Neutrophils and Dendritic Cells in Behcet’s Disease”. Clinical and Experimental Health Sciences 2, no. 6 (February 2014).
EndNote	Türe Özdemir F, Tulunay A, Elbaşı MO, Tatlı İ, Maurer A-M, Mumcu G, Direskeneli H, Ekşioğlu Demiralp E (February 1, 2014) Pro-Inflammatory Cytokine and Caspase-1 Responses to Pattern Recognition Receptor Activation of Neutrophils and Dendritic Cells in Behcet’s Disease. Clinical and Experimental Health Sciences 2 6
IEEE	F. Türe Özdemir, A. Tulunay, M. O. Elbaşı, İ. Tatlı, A.-M. Maurer, G. Mumcu, H. Direskeneli, and E. Ekşioğlu Demiralp, “Pro-Inflammatory Cytokine and Caspase-1 Responses to Pattern Recognition Receptor Activation of Neutrophils and Dendritic Cells in Behcet’s Disease”, Clinical and Experimental Health Sciences, vol. 2, no. 6, 2014.
ISNAD	Türe Özdemir, Filiz et al. “Pro-Inflammatory Cytokine and Caspase-1 Responses to Pattern Recognition Receptor Activation of Neutrophils and Dendritic Cells in Behcet’s Disease”. Clinical and Experimental Health Sciences 2/6 (February 2014).
JAMA	Türe Özdemir F, Tulunay A, Elbaşı MO, Tatlı İ, Maurer A-M, Mumcu G, Direskeneli H, Ekşioğlu Demiralp E. Pro-Inflammatory Cytokine and Caspase-1 Responses to Pattern Recognition Receptor Activation of Neutrophils and Dendritic Cells in Behcet’s Disease. Clinical and Experimental Health Sciences. 2014;2.
MLA	Türe Özdemir, Filiz et al. “Pro-Inflammatory Cytokine and Caspase-1 Responses to Pattern Recognition Receptor Activation of Neutrophils and Dendritic Cells in Behcet’s Disease”. Clinical and Experimental Health Sciences, vol. 2, no. 6, 2014.
Vancouver	Türe Özdemir F, Tulunay A, Elbaşı MO, Tatlı İ, Maurer A-M, Mumcu G, Direskeneli H, Ekşioğlu Demiralp E. Pro-Inflammatory Cytokine and Caspase-1 Responses to Pattern Recognition Receptor Activation of Neutrophils and Dendritic Cells in Behcet’s Disease. Clinical and Experimental Health Sciences. 2014;2(6).