Effect of dietary n-3 series fatty acids on sperm motility duration of rainbow trout (Oncorhynchus mykiss Walbaum, 1792)

Yıl 2020, Cilt: 10 Sayı: 2, 104 - 107, 29.06.2020

Şeyma Özer Kaya , Seyfettin Gür , Kenan Köprücü , Gaffari Türk , Mustafa Sönmez , Sinan Ozcan

https://doi.org/10.33808/clinexphealthsci.687305

Öz

Objective: This study was performed to determine the effect of different ratios of n-3 series fatty acids added to the diets of rainbow trout

(Oncorhynchus mykiss) on sperm motility duration.

Methods: A total of 48 male rainbow trouts about 3 years of age were used in the study and 4 groups were formed. The one that n-3 series fatty

acids were not added to the diet was used as control group. n-3 series fatty acids were added to the experimental groups diets at the ratios

of 1% (E1), 2% (E2) and 3% (E3), respectively. Semen was collected by abdominal massage. The time-dependent sperm motility change was

detected under the microscope at x400 magnification.

Results: A significant decrease was detected in the motility only at 360th hour in E3 group compared to the control group (p<0.05).

Conclusion: As a result, it was concluded that feeding with diets containing different ratios of n-3 series fatty acids had no effect on the motility

of rainbow trout semen under in vitro condition.

Anahtar Kelimeler

Diet, Duration of Motility, Fatty Acid, Rainbow Trout, Sperm Motility

Kaynakça

• 1. Çelikkale MS. İçsu Balıkları ve Yetiştiriciliği, Karadeniz Teknik Üniversitesi, Sürmene Deniz Bilimleri ve Teknolojisi Yüksekokulu, Fakülte Yayın No: 2, Trabzon, 1988, 419.
• 2. Dreanno C, Cosson J, Suquet M, Seguin F, Dorange G, Billard R. Nucleotide content, oxidative phosphorylation, morphology and fertilizing capacity of turbot (psetta maxima) spermatozoa during the motility period. Mol Reprod Dev 1999; 53:230-243.
• 3. Okumuş İ. Rainbow trout broodstock management and seed production in turkey: present practices, constraints and the future. Turkish J Fish Aqua Sci 2003; 2:41-56.
• 4. Yavuz H. Farklı Kromozomlara (XX ve XY) Sahip Erkek Gökkuşağı Alabalığı (Oncorhynchus mykiss) Spermlerinin Dölleme Kalitesi. Yüksek Lisans Tezi Fırat Üniversitesi, Elazığ. 2012.
• 5. Cosson J. Fish Sperm Physiology: Structure, Factors Regulating Motility, and Motility Evaluation. In Recent Developments in Fish Biology Researches. 2019: 1-26.
• 6. Sargent JR, Toche DR, Bell JG. The Lipids. In: Fish Nutrition, Halver JE & Hardy RW (Ed), 3rd ed., Academic Press, San Diego, 2002; 182-257.
• 7. Goddard S. Feed Management in Intensive Aquaculture, Printed in the United States of America, Includes bibliographical references and index. Chapman and Hall Press, New York. 1996.
• 8. Mattson NS, Riple TH. Metomidate, a better anaesthetic for cod (Gadus morhua) in comparation with benzocain, MS-222, choloro butanol and phenoxyethanol, Aquaculture 1989; 83:89-94.
• 9. Munkittrick KR, Moccia RD. Seasonal changes in the quality of rainbow trout (Salmo gairdneri) semen: Effect of a delay in stripping on sprmatocrit, motility, volume and seminal plasma constituents. Aquaculture, 1987; 64:147-156.
• 10. National Research Council (NRC). Nutrient Requirements of Fish, National Academy Press, Washington DC. 1999.
• 11. Lahnsteiner F, Mansour N, McNiven M A, Richrdson GF. Fatty acids of rainbow trout (Oncorhynchus mykiss) semen: Composition and effects on sperm functionality. Aquaculture 2009; 298:118-124.
• 12. Huang X, Yin Y, Shi Z, Li W, Zhou H, Lv W. Lipid content and fatty acid composition in wild-caught silver pomfret (Pampus argenteus) broodstocks: effects on gonad development. Aquaculture 2010; 310:192-199.
• 13. Aas GH, Refstie T, Gjerde B. Evaluation of milt quality of atlantic salmon. Aquaculture 1991; 95:125-132.
• 14. Bromage Niall R, Ronald J. Broodstock management and egg larval quality. Wiley-Blackwell Science Ltd, Cambridge. 1995.
• 15. Benau D, Terner C. Initiation prolongation and reactivation of the motility of salmonid spermatozoa. Gamete Res 1980; 3:247-257.
• 16. Boitano S, Omoto CK. Membrane hyperpolarization activates trout sperm without an increase in intracellular pH. J Cell Sci 1991; 98:343-349.
• 17. Chambeyron F, Zohar Y. A diluent for sperm cryopreservation of gilthead seabream, Sparus aurata. Aquaculture 1990; 90:345-352.
• 18. Izquierdo MS, Fernandez-Palacios H, Tacon AGJ. Effect of broodstock nutrition on reproductive performance of fish. Aquaculture 2001; 197:25-42.
• 19. Vassallo-Agius R, Watanabe Takeshi Yoshizaki G, Satoh S, Takeuchi Y. Quality of eggs and spermatozoa of rainbow trout fed an n-3 essential fatty acid-deficient diet and its effects on the lipid and fatty acid components of eggs, semen and livers. Fisheries Sci 2001; 67:818-827.
• 20. Frøyland L, Lie Ø, Berge RK (). Mitochondrial and peroxisomal β-oxidation capacities in various tissues from atlantic salmon salmo salar. Aquacult Nutr 2000; 6:85-89.
• 21. Asturiano JF, Sorbera LA, Carrillo M, et al. Reproductive performance in male European sea bass (Dicentrarchus labrax, L.) fed two PUFAenriched experimental diets: a comparison with males fed a wet diet. Aquaculture 2001; 194:173–190.
• 22. Alavi SMH, Psenicka M, Polica T, et al. Sperm quality in male Barbus barbus L. fed different diets during the spawning season. Fish Physiol Biochem 2009; 35:683-693.