Alterations in Enzyme Activity of Carbonic Anhydrase, 6-phosphogluconate Dehydrogenase and Thioredoxin Reductase in Rats Exposed to Doxorubicin and Morin

Year 2020, Volume: 10 Issue: 3, 228 - 234, 29.09.2020

Ahmet Gökhan Ağgül , Müslüm Kuzu , Fatih Mehmet Kandemir Sefa Küçükler , Cüneyt Çağlayan

Abstract

Objective:
Carbonic anhydrase (CA), 6-phosphogluconate dehydrogenase (6PGD) and
thioredoxin reductase (TrxR) enzymes are the essential biological molecules needed for metabolic processes in all living
cells. This study was designed to investigate the activities of CA, 6PGD and
TrxR enzymes in the brain, kidney, liver, heart and testis tissues of the rats
exposed to Dox and morin.

Methods:
Male Wistar albino rats were randomly divided into three groups as control,
morin and DOX, each of them containing 7 rats. At the end of the experimental
procedure, CA, 6PGD and TrxR enzyme activities in tissues of rats were
determined spectrophotometrically.

Results:
In our study, we observed that DOX activated CA enzyme in liver and kidney
tissues while inhibiting CA enzyme in the other tissues, activated 6PGD enzyme in the kidney, liver and heart
tissues, and inhibited the TrxR enzyme in all the tissues. In addition, morin
activated CA enzyme in the liver tissue while inhibiting CA enzyme in the
brain, heart and testis tissues. Morin activated 6PGD enzyme activity while it inhibited TrxR enzyme in all the tissues.

Conclusion:
The findings showed that doxorubicin and morin had similar properties in the
tissues as to their effect on enzyme activities.

Keywords

Doxorubicin, Morin, Carbonic Anhydrase, 6-phosphogluconate Dehydrogenase, Thioredoxin Reductase

Project Number

ECZF.17.001

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